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Differentiation Potential of Pancreatic Fibroblastoid Cells/Stellate Cells: Effects of Peroxisome Proliferator-Activated Receptor Gamma Ligands

Pancreatic stellate cells have been investigated mostly for their activation process, supposed to support the development of pancreatic disease. Few studies have been presented on reversal of the activation process in vitro. Thiazolidinediones (TZDs) have been used as antidiabetics and have now been...

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Autores principales: Kruse, M.-L., Hopf-Jensen, S., Timke, C., Agricola, B., Sparmann, G., Schmid, A., Sipos, B., Arlt, A., Schäfer, H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189590/
https://www.ncbi.nlm.nih.gov/pubmed/22007221
http://dx.doi.org/10.1155/2011/816791
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author Kruse, M.-L.
Hopf-Jensen, S.
Timke, C.
Agricola, B.
Sparmann, G.
Schmid, A.
Sipos, B.
Arlt, A.
Schäfer, H.
author_facet Kruse, M.-L.
Hopf-Jensen, S.
Timke, C.
Agricola, B.
Sparmann, G.
Schmid, A.
Sipos, B.
Arlt, A.
Schäfer, H.
author_sort Kruse, M.-L.
collection PubMed
description Pancreatic stellate cells have been investigated mostly for their activation process, supposed to support the development of pancreatic disease. Few studies have been presented on reversal of the activation process in vitro. Thiazolidinediones (TZDs) have been used as antidiabetics and have now been reported to exert antifibrotic activity. We tested effects of natural and synthetic ligands of peroxisome proliferator-activated receptor gamma (PPARγ) on human pancreatic fibroblastoid cells (hPFCs) in search for specificity of action. Ciglitazone, as a prototype of TZDs, was shown to have reversible growth inhibitory effects on human pancreatic fibroblastoid cells/stellate cells. Cells treated with ciglitazone for three days showed enhanced lipid content and induction of proteins involved in lipid metabolism. Collagen synthesis was reduced in hPFC. Interaction of PPARγ with DNA binding sites upon ligand binding was shown by gel shift analysis. These findings point toward a potential for adipocyte differentiation in human pancreatic fibroblastoid cells.
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spelling pubmed-31895902011-10-17 Differentiation Potential of Pancreatic Fibroblastoid Cells/Stellate Cells: Effects of Peroxisome Proliferator-Activated Receptor Gamma Ligands Kruse, M.-L. Hopf-Jensen, S. Timke, C. Agricola, B. Sparmann, G. Schmid, A. Sipos, B. Arlt, A. Schäfer, H. Int J Cell Biol Research Article Pancreatic stellate cells have been investigated mostly for their activation process, supposed to support the development of pancreatic disease. Few studies have been presented on reversal of the activation process in vitro. Thiazolidinediones (TZDs) have been used as antidiabetics and have now been reported to exert antifibrotic activity. We tested effects of natural and synthetic ligands of peroxisome proliferator-activated receptor gamma (PPARγ) on human pancreatic fibroblastoid cells (hPFCs) in search for specificity of action. Ciglitazone, as a prototype of TZDs, was shown to have reversible growth inhibitory effects on human pancreatic fibroblastoid cells/stellate cells. Cells treated with ciglitazone for three days showed enhanced lipid content and induction of proteins involved in lipid metabolism. Collagen synthesis was reduced in hPFC. Interaction of PPARγ with DNA binding sites upon ligand binding was shown by gel shift analysis. These findings point toward a potential for adipocyte differentiation in human pancreatic fibroblastoid cells. Hindawi Publishing Corporation 2011 2011-10-04 /pmc/articles/PMC3189590/ /pubmed/22007221 http://dx.doi.org/10.1155/2011/816791 Text en Copyright © 2011 M.-L. Kruse et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kruse, M.-L.
Hopf-Jensen, S.
Timke, C.
Agricola, B.
Sparmann, G.
Schmid, A.
Sipos, B.
Arlt, A.
Schäfer, H.
Differentiation Potential of Pancreatic Fibroblastoid Cells/Stellate Cells: Effects of Peroxisome Proliferator-Activated Receptor Gamma Ligands
title Differentiation Potential of Pancreatic Fibroblastoid Cells/Stellate Cells: Effects of Peroxisome Proliferator-Activated Receptor Gamma Ligands
title_full Differentiation Potential of Pancreatic Fibroblastoid Cells/Stellate Cells: Effects of Peroxisome Proliferator-Activated Receptor Gamma Ligands
title_fullStr Differentiation Potential of Pancreatic Fibroblastoid Cells/Stellate Cells: Effects of Peroxisome Proliferator-Activated Receptor Gamma Ligands
title_full_unstemmed Differentiation Potential of Pancreatic Fibroblastoid Cells/Stellate Cells: Effects of Peroxisome Proliferator-Activated Receptor Gamma Ligands
title_short Differentiation Potential of Pancreatic Fibroblastoid Cells/Stellate Cells: Effects of Peroxisome Proliferator-Activated Receptor Gamma Ligands
title_sort differentiation potential of pancreatic fibroblastoid cells/stellate cells: effects of peroxisome proliferator-activated receptor gamma ligands
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189590/
https://www.ncbi.nlm.nih.gov/pubmed/22007221
http://dx.doi.org/10.1155/2011/816791
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