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Multilocus Phylogenetics Show High Intraspecific Variability within Fusarium avenaceum

Fusarium avenaceum is a common soil saprophyte and plant pathogen of a variety of hosts worldwide. This pathogen is often involved in the crown rot and head blight of cereals that affects grain yield and quality. F. avenaceum contaminates grain with enniatins more than any species, and they are ofte...

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Autores principales: Kulik, Tomasz, Pszczółkowska, Agnieszka, Łojko, Maciej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189738/
https://www.ncbi.nlm.nih.gov/pubmed/22016614
http://dx.doi.org/10.3390/ijms12095626
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author Kulik, Tomasz
Pszczółkowska, Agnieszka
Łojko, Maciej
author_facet Kulik, Tomasz
Pszczółkowska, Agnieszka
Łojko, Maciej
author_sort Kulik, Tomasz
collection PubMed
description Fusarium avenaceum is a common soil saprophyte and plant pathogen of a variety of hosts worldwide. This pathogen is often involved in the crown rot and head blight of cereals that affects grain yield and quality. F. avenaceum contaminates grain with enniatins more than any species, and they are often detected at the highest prevalence among fusarial toxins in certain geographic areas. We studied intraspecific variability of F. avenaceum based on partial sequences of elongation factor-1 alpha, enniatin synthase, intergenic spacer of rDNA, arylamine N-acetyltransferase and RNA polymerase II data sets. The phylogenetic analyses incorporated a collection of 63 F. avenaceum isolates of various origin among which 41 were associated with wheat. Analyses of the multilocus sequence (MLS) data indicated a high level of genetic variation within the isolates studied with no significant linkage disequilibrium. Correspondingly, maximum parsimony analyses of both MLS and individual data sets showed lack of clear phylogenetic structure within F. avenaceum in relation to host (wheat) and geographic origin. Lack of host specialization indicates no host selective pressure in driving F. avenaceum evolution, while no geographic lineage structure indicates widespread distribution of genotypes that resulted in nullifying the effects of geographic isolation on the evolution of this species. Moreover, significant incongruence between all individual tree topologies and little clonality is consistent with frequent recombination within F. avenaceum.
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spelling pubmed-31897382011-10-20 Multilocus Phylogenetics Show High Intraspecific Variability within Fusarium avenaceum Kulik, Tomasz Pszczółkowska, Agnieszka Łojko, Maciej Int J Mol Sci Article Fusarium avenaceum is a common soil saprophyte and plant pathogen of a variety of hosts worldwide. This pathogen is often involved in the crown rot and head blight of cereals that affects grain yield and quality. F. avenaceum contaminates grain with enniatins more than any species, and they are often detected at the highest prevalence among fusarial toxins in certain geographic areas. We studied intraspecific variability of F. avenaceum based on partial sequences of elongation factor-1 alpha, enniatin synthase, intergenic spacer of rDNA, arylamine N-acetyltransferase and RNA polymerase II data sets. The phylogenetic analyses incorporated a collection of 63 F. avenaceum isolates of various origin among which 41 were associated with wheat. Analyses of the multilocus sequence (MLS) data indicated a high level of genetic variation within the isolates studied with no significant linkage disequilibrium. Correspondingly, maximum parsimony analyses of both MLS and individual data sets showed lack of clear phylogenetic structure within F. avenaceum in relation to host (wheat) and geographic origin. Lack of host specialization indicates no host selective pressure in driving F. avenaceum evolution, while no geographic lineage structure indicates widespread distribution of genotypes that resulted in nullifying the effects of geographic isolation on the evolution of this species. Moreover, significant incongruence between all individual tree topologies and little clonality is consistent with frequent recombination within F. avenaceum. Molecular Diversity Preservation International (MDPI) 2011-08-31 /pmc/articles/PMC3189738/ /pubmed/22016614 http://dx.doi.org/10.3390/ijms12095626 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Kulik, Tomasz
Pszczółkowska, Agnieszka
Łojko, Maciej
Multilocus Phylogenetics Show High Intraspecific Variability within Fusarium avenaceum
title Multilocus Phylogenetics Show High Intraspecific Variability within Fusarium avenaceum
title_full Multilocus Phylogenetics Show High Intraspecific Variability within Fusarium avenaceum
title_fullStr Multilocus Phylogenetics Show High Intraspecific Variability within Fusarium avenaceum
title_full_unstemmed Multilocus Phylogenetics Show High Intraspecific Variability within Fusarium avenaceum
title_short Multilocus Phylogenetics Show High Intraspecific Variability within Fusarium avenaceum
title_sort multilocus phylogenetics show high intraspecific variability within fusarium avenaceum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189738/
https://www.ncbi.nlm.nih.gov/pubmed/22016614
http://dx.doi.org/10.3390/ijms12095626
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