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Bioactive Compounds and Biological Activities of Jatropha curcas L. Kernel Meal Extract
Defatted Jatropha curcas L. (J. curcas) seed kernels contained a high percentage of crude protein (61.8%) and relatively little acid detergent fiber (4.8%) and neutral detergent fiber (9.7%). Spectrophotometric analysis of the methanolic extract showed the presence of phenolics, flavonoids and sapon...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189762/ https://www.ncbi.nlm.nih.gov/pubmed/22016638 http://dx.doi.org/10.3390/ijms12095955 |
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author | Oskoueian, Ehsan Abdullah, Norhani Ahmad, Syahida Saad, Wan Zuhainis Omar, Abdul Rahman Ho, Yin Wan |
author_facet | Oskoueian, Ehsan Abdullah, Norhani Ahmad, Syahida Saad, Wan Zuhainis Omar, Abdul Rahman Ho, Yin Wan |
author_sort | Oskoueian, Ehsan |
collection | PubMed |
description | Defatted Jatropha curcas L. (J. curcas) seed kernels contained a high percentage of crude protein (61.8%) and relatively little acid detergent fiber (4.8%) and neutral detergent fiber (9.7%). Spectrophotometric analysis of the methanolic extract showed the presence of phenolics, flavonoids and saponins with values of 3.9, 0.4 and 19.0 mg/g DM, respectively. High performance liquid chromatography (HPLC) analyses showed the presence of gallic acid and pyrogallol (phenolics), rutin and myricetin (flavonoids) and daidzein (isoflavonoid). The amount of phorbol esters in the methanolic extract estimated by HPLC was 3.0 ± 0.1 mg/g DM. Other metabolites detected by GC-MS include: 2-(hydroxymethyl)-2 nitro-1,3-propanediol, β-sitosterol, 2-furancarboxaldehyde, 5-(hydroxymethy) and acetic acid in the methanolic extract; 2-furancarboxaldehyde, 5-(hydroxymethy), acetic acid and furfural (2-furancarboxaldehyde) in the hot water extract. Methanolic and hot water extracts of kernel meal showed antimicrobial activity against both Gram positive and Gram negative pathogenic bacteria (inhibition range: 0–1.63 cm) at the concentrations of 1 and 1.5 mg/disc. Methanolic extract exhibited antioxidant activities that are higher than hot water extract and comparable to β-carotene. The extracts tended to scavenge the free radicals in the reduction of ferric ion (Fe(3+)) to ferrous ion (Fe(2+)). Cytotoxicity assay results indicated the potential of methanolic extract as a source of anticancer therapeutic agents toward breast cancer cells. |
format | Online Article Text |
id | pubmed-3189762 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-31897622011-10-20 Bioactive Compounds and Biological Activities of Jatropha curcas L. Kernel Meal Extract Oskoueian, Ehsan Abdullah, Norhani Ahmad, Syahida Saad, Wan Zuhainis Omar, Abdul Rahman Ho, Yin Wan Int J Mol Sci Article Defatted Jatropha curcas L. (J. curcas) seed kernels contained a high percentage of crude protein (61.8%) and relatively little acid detergent fiber (4.8%) and neutral detergent fiber (9.7%). Spectrophotometric analysis of the methanolic extract showed the presence of phenolics, flavonoids and saponins with values of 3.9, 0.4 and 19.0 mg/g DM, respectively. High performance liquid chromatography (HPLC) analyses showed the presence of gallic acid and pyrogallol (phenolics), rutin and myricetin (flavonoids) and daidzein (isoflavonoid). The amount of phorbol esters in the methanolic extract estimated by HPLC was 3.0 ± 0.1 mg/g DM. Other metabolites detected by GC-MS include: 2-(hydroxymethyl)-2 nitro-1,3-propanediol, β-sitosterol, 2-furancarboxaldehyde, 5-(hydroxymethy) and acetic acid in the methanolic extract; 2-furancarboxaldehyde, 5-(hydroxymethy), acetic acid and furfural (2-furancarboxaldehyde) in the hot water extract. Methanolic and hot water extracts of kernel meal showed antimicrobial activity against both Gram positive and Gram negative pathogenic bacteria (inhibition range: 0–1.63 cm) at the concentrations of 1 and 1.5 mg/disc. Methanolic extract exhibited antioxidant activities that are higher than hot water extract and comparable to β-carotene. The extracts tended to scavenge the free radicals in the reduction of ferric ion (Fe(3+)) to ferrous ion (Fe(2+)). Cytotoxicity assay results indicated the potential of methanolic extract as a source of anticancer therapeutic agents toward breast cancer cells. Molecular Diversity Preservation International (MDPI) 2011-09-15 /pmc/articles/PMC3189762/ /pubmed/22016638 http://dx.doi.org/10.3390/ijms12095955 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Oskoueian, Ehsan Abdullah, Norhani Ahmad, Syahida Saad, Wan Zuhainis Omar, Abdul Rahman Ho, Yin Wan Bioactive Compounds and Biological Activities of Jatropha curcas L. Kernel Meal Extract |
title | Bioactive Compounds and Biological Activities of Jatropha curcas L. Kernel Meal Extract |
title_full | Bioactive Compounds and Biological Activities of Jatropha curcas L. Kernel Meal Extract |
title_fullStr | Bioactive Compounds and Biological Activities of Jatropha curcas L. Kernel Meal Extract |
title_full_unstemmed | Bioactive Compounds and Biological Activities of Jatropha curcas L. Kernel Meal Extract |
title_short | Bioactive Compounds and Biological Activities of Jatropha curcas L. Kernel Meal Extract |
title_sort | bioactive compounds and biological activities of jatropha curcas l. kernel meal extract |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189762/ https://www.ncbi.nlm.nih.gov/pubmed/22016638 http://dx.doi.org/10.3390/ijms12095955 |
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