Isolation and Characterization of Sixteen Polymorphic Microsatellite Loci in the Golden Apple Snail Pomacea canaliculata

We report the characterization of 16 polymorphic microsatellite markers in the golden apple snail, Pomacea canaliculata, a pest registered in the list of “100 of the world’s worst invasive alien species”. The fast isolation by AFLP (Amplified Fragment Length Polymorphism) of sequences containing rep...

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Detalles Bibliográficos
Autores principales: Chen, Lian, Xu, Haigen, Li, Hong, Wu, Jun, Ding, Hui, Liu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189764/
https://www.ncbi.nlm.nih.gov/pubmed/22016640
http://dx.doi.org/10.3390/ijms12095993
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author Chen, Lian
Xu, Haigen
Li, Hong
Wu, Jun
Ding, Hui
Liu, Yan
author_facet Chen, Lian
Xu, Haigen
Li, Hong
Wu, Jun
Ding, Hui
Liu, Yan
author_sort Chen, Lian
collection PubMed
description We report the characterization of 16 polymorphic microsatellite markers in the golden apple snail, Pomacea canaliculata, a pest registered in the list of “100 of the world’s worst invasive alien species”. The fast isolation by AFLP (Amplified Fragment Length Polymorphism) of sequences containing repeats (FIASCO) method was used to isolate microsatellite loci, and polymorphism was explored with 29 individuals collected in an invasive region from China. These primers showed a number of alleles per locus ranging from three to 13. The ranges of observed and expected heterozygosity were 0.310–0.966 and 0.523–0.898, respectively. These microsatellite markers described here will be useful for population genetic studies of P. canaliculata.
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spelling pubmed-31897642011-10-20 Isolation and Characterization of Sixteen Polymorphic Microsatellite Loci in the Golden Apple Snail Pomacea canaliculata Chen, Lian Xu, Haigen Li, Hong Wu, Jun Ding, Hui Liu, Yan Int J Mol Sci Article We report the characterization of 16 polymorphic microsatellite markers in the golden apple snail, Pomacea canaliculata, a pest registered in the list of “100 of the world’s worst invasive alien species”. The fast isolation by AFLP (Amplified Fragment Length Polymorphism) of sequences containing repeats (FIASCO) method was used to isolate microsatellite loci, and polymorphism was explored with 29 individuals collected in an invasive region from China. These primers showed a number of alleles per locus ranging from three to 13. The ranges of observed and expected heterozygosity were 0.310–0.966 and 0.523–0.898, respectively. These microsatellite markers described here will be useful for population genetic studies of P. canaliculata. Molecular Diversity Preservation International (MDPI) 2011-09-16 /pmc/articles/PMC3189764/ /pubmed/22016640 http://dx.doi.org/10.3390/ijms12095993 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Chen, Lian
Xu, Haigen
Li, Hong
Wu, Jun
Ding, Hui
Liu, Yan
Isolation and Characterization of Sixteen Polymorphic Microsatellite Loci in the Golden Apple Snail Pomacea canaliculata
title Isolation and Characterization of Sixteen Polymorphic Microsatellite Loci in the Golden Apple Snail Pomacea canaliculata
title_full Isolation and Characterization of Sixteen Polymorphic Microsatellite Loci in the Golden Apple Snail Pomacea canaliculata
title_fullStr Isolation and Characterization of Sixteen Polymorphic Microsatellite Loci in the Golden Apple Snail Pomacea canaliculata
title_full_unstemmed Isolation and Characterization of Sixteen Polymorphic Microsatellite Loci in the Golden Apple Snail Pomacea canaliculata
title_short Isolation and Characterization of Sixteen Polymorphic Microsatellite Loci in the Golden Apple Snail Pomacea canaliculata
title_sort isolation and characterization of sixteen polymorphic microsatellite loci in the golden apple snail pomacea canaliculata
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189764/
https://www.ncbi.nlm.nih.gov/pubmed/22016640
http://dx.doi.org/10.3390/ijms12095993
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