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Improving Protein Crystal Quality by the Without-Oil Microbatch Method: Crystallization and Preliminary X-ray Diffraction Analysis of Glutathione Synthetase from Pseudoalteromonas haloplanktis
Glutathione synthetases catalyze the ATP-dependent synthesis of glutathione from l-γ-glutamyl- l-cysteine and glycine. Although these enzymes have been sequenced and characterized from a variety of biological sources, their exact catalytic mechanism is not fully understood and nothing is known about...
Autores principales: | Merlino, Antonello, Krauss, Irene Russo, Albino, Antonella, Pica, Andrea, Vergara, Alessandro, Masullo, Mariorosario, De Vendittis, Emmanuele, Sica, Filomena |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189784/ https://www.ncbi.nlm.nih.gov/pubmed/22016660 http://dx.doi.org/10.3390/ijms12096312 |
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