Cargando…
Intracellular ROS Protection Efficiency and Free Radical-Scavenging Activity of Curcumin
Curcumin has many pharmaceutical applications, many of which arise from its potent antioxidant properties. The present research examined the antioxidant activities of curcumin in polar solvents by a comparative study using ESR, reduction of ferric iron in aqueous medium and intracellular ROS/toxicit...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189944/ https://www.ncbi.nlm.nih.gov/pubmed/22016801 http://dx.doi.org/10.1371/journal.pone.0026012 |
_version_ | 1782213531822718976 |
---|---|
author | Barzegar, Abolfazl Moosavi-Movahedi, Ali A. |
author_facet | Barzegar, Abolfazl Moosavi-Movahedi, Ali A. |
author_sort | Barzegar, Abolfazl |
collection | PubMed |
description | Curcumin has many pharmaceutical applications, many of which arise from its potent antioxidant properties. The present research examined the antioxidant activities of curcumin in polar solvents by a comparative study using ESR, reduction of ferric iron in aqueous medium and intracellular ROS/toxicity assays. ESR data indicated that the steric hindrance among adjacent big size groups within a galvinoxyl molecule limited the curcumin to scavenge galvinoxyl radicals effectively, while curcumin showed a powerful capacity for scavenging intracellular smaller oxidative molecules such as H(2)O(2), HO(•), ROO(•). Cell viability and ROS assays demonstrated that curcumin was able to penetrate into the polar medium inside the cells and to protect them against the highly toxic and lethal effects of cumene hydroperoxide. Curcumin also showed good electron-transfer capability, with greater activity than trolox in aqueous solution. Curcumin can readily transfer electron or easily donate H-atom from two phenolic sites to scavenge free radicals. The excellent electron transfer capability of curcumin is because of its unique structure and different functional groups, including a β-diketone and several π electrons that have the capacity to conjugate between two phenyl rings. Therfore, since curcumin is inherently a lipophilic compound, because of its superb intracellular ROS scavenging activity, it can be used as an effective antioxidant for ROS protection within the polar cytoplasm. |
format | Online Article Text |
id | pubmed-3189944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31899442011-10-20 Intracellular ROS Protection Efficiency and Free Radical-Scavenging Activity of Curcumin Barzegar, Abolfazl Moosavi-Movahedi, Ali A. PLoS One Research Article Curcumin has many pharmaceutical applications, many of which arise from its potent antioxidant properties. The present research examined the antioxidant activities of curcumin in polar solvents by a comparative study using ESR, reduction of ferric iron in aqueous medium and intracellular ROS/toxicity assays. ESR data indicated that the steric hindrance among adjacent big size groups within a galvinoxyl molecule limited the curcumin to scavenge galvinoxyl radicals effectively, while curcumin showed a powerful capacity for scavenging intracellular smaller oxidative molecules such as H(2)O(2), HO(•), ROO(•). Cell viability and ROS assays demonstrated that curcumin was able to penetrate into the polar medium inside the cells and to protect them against the highly toxic and lethal effects of cumene hydroperoxide. Curcumin also showed good electron-transfer capability, with greater activity than trolox in aqueous solution. Curcumin can readily transfer electron or easily donate H-atom from two phenolic sites to scavenge free radicals. The excellent electron transfer capability of curcumin is because of its unique structure and different functional groups, including a β-diketone and several π electrons that have the capacity to conjugate between two phenyl rings. Therfore, since curcumin is inherently a lipophilic compound, because of its superb intracellular ROS scavenging activity, it can be used as an effective antioxidant for ROS protection within the polar cytoplasm. Public Library of Science 2011-10-10 /pmc/articles/PMC3189944/ /pubmed/22016801 http://dx.doi.org/10.1371/journal.pone.0026012 Text en Barzegar, Moosavi-Movahedi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Barzegar, Abolfazl Moosavi-Movahedi, Ali A. Intracellular ROS Protection Efficiency and Free Radical-Scavenging Activity of Curcumin |
title | Intracellular ROS Protection Efficiency and Free Radical-Scavenging Activity of Curcumin |
title_full | Intracellular ROS Protection Efficiency and Free Radical-Scavenging Activity of Curcumin |
title_fullStr | Intracellular ROS Protection Efficiency and Free Radical-Scavenging Activity of Curcumin |
title_full_unstemmed | Intracellular ROS Protection Efficiency and Free Radical-Scavenging Activity of Curcumin |
title_short | Intracellular ROS Protection Efficiency and Free Radical-Scavenging Activity of Curcumin |
title_sort | intracellular ros protection efficiency and free radical-scavenging activity of curcumin |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189944/ https://www.ncbi.nlm.nih.gov/pubmed/22016801 http://dx.doi.org/10.1371/journal.pone.0026012 |
work_keys_str_mv | AT barzegarabolfazl intracellularrosprotectionefficiencyandfreeradicalscavengingactivityofcurcumin AT moosavimovahedialia intracellularrosprotectionefficiencyandfreeradicalscavengingactivityofcurcumin |