Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea
Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR (rep-PCR)-based DiversiLab™ system (bioMérieux, France) over the IS6110...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Laboratory Medicine
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3190008/ https://www.ncbi.nlm.nih.gov/pubmed/22016683 http://dx.doi.org/10.3343/kjlm.2011.31.4.282 |
_version_ | 1782213539717447680 |
---|---|
author | Jang, Mi Hee Choi, Go Eun Shin, Bo-Moon Lee, Seon Ho Kim, Sung-Ryul Chang, Chulhun L. Kim, Jeong-Man |
author_facet | Jang, Mi Hee Choi, Go Eun Shin, Bo-Moon Lee, Seon Ho Kim, Sung-Ryul Chang, Chulhun L. Kim, Jeong-Man |
author_sort | Jang, Mi Hee |
collection | PubMed |
description | Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR (rep-PCR)-based DiversiLab™ system (bioMérieux, France) over the IS6110-restriction fragment length polymorphism analysis for detecting Mycobacterium tuberculosis. In all, 89 clinical M. tuberculosis isolates collected nationwide from Korea were used. The DiversiLab system allocated the 89 isolates to 8 groups with 1 unique isolate when a similarity level of 95% was applied. Seventy-six isolates of the Beijing family and 13 isolates of non-Beijing family strains were irregularly distributed regardless of rep-PCR groups. The DiversiLab system generated a rapid, sensitive, and standardized result. It can be used to conduct molecular epidemiologic studies to identify clinical M. tuberculosis isolates in Korea. |
format | Online Article Text |
id | pubmed-3190008 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Korean Society for Laboratory Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-31900082011-10-20 Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea Jang, Mi Hee Choi, Go Eun Shin, Bo-Moon Lee, Seon Ho Kim, Sung-Ryul Chang, Chulhun L. Kim, Jeong-Man Korean J Lab Med Brief Communication Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR (rep-PCR)-based DiversiLab™ system (bioMérieux, France) over the IS6110-restriction fragment length polymorphism analysis for detecting Mycobacterium tuberculosis. In all, 89 clinical M. tuberculosis isolates collected nationwide from Korea were used. The DiversiLab system allocated the 89 isolates to 8 groups with 1 unique isolate when a similarity level of 95% was applied. Seventy-six isolates of the Beijing family and 13 isolates of non-Beijing family strains were irregularly distributed regardless of rep-PCR groups. The DiversiLab system generated a rapid, sensitive, and standardized result. It can be used to conduct molecular epidemiologic studies to identify clinical M. tuberculosis isolates in Korea. The Korean Society for Laboratory Medicine 2011-10 2011-10-03 /pmc/articles/PMC3190008/ /pubmed/22016683 http://dx.doi.org/10.3343/kjlm.2011.31.4.282 Text en Copyright © 2011 The Korean Society for Laboratory Medicine http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Brief Communication Jang, Mi Hee Choi, Go Eun Shin, Bo-Moon Lee, Seon Ho Kim, Sung-Ryul Chang, Chulhun L. Kim, Jeong-Man Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea |
title | Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea |
title_full | Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea |
title_fullStr | Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea |
title_full_unstemmed | Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea |
title_short | Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea |
title_sort | comparison of an automated repetitive sequence-based pcr microbial typing system with is6110-restriction fragment length polymorphism for epidemiologic investigation of clinical mycobacterium tuberculosis isolates in korea |
topic | Brief Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3190008/ https://www.ncbi.nlm.nih.gov/pubmed/22016683 http://dx.doi.org/10.3343/kjlm.2011.31.4.282 |
work_keys_str_mv | AT jangmihee comparisonofanautomatedrepetitivesequencebasedpcrmicrobialtypingsystemwithis6110restrictionfragmentlengthpolymorphismforepidemiologicinvestigationofclinicalmycobacteriumtuberculosisisolatesinkorea AT choigoeun comparisonofanautomatedrepetitivesequencebasedpcrmicrobialtypingsystemwithis6110restrictionfragmentlengthpolymorphismforepidemiologicinvestigationofclinicalmycobacteriumtuberculosisisolatesinkorea AT shinbomoon comparisonofanautomatedrepetitivesequencebasedpcrmicrobialtypingsystemwithis6110restrictionfragmentlengthpolymorphismforepidemiologicinvestigationofclinicalmycobacteriumtuberculosisisolatesinkorea AT leeseonho comparisonofanautomatedrepetitivesequencebasedpcrmicrobialtypingsystemwithis6110restrictionfragmentlengthpolymorphismforepidemiologicinvestigationofclinicalmycobacteriumtuberculosisisolatesinkorea AT kimsungryul comparisonofanautomatedrepetitivesequencebasedpcrmicrobialtypingsystemwithis6110restrictionfragmentlengthpolymorphismforepidemiologicinvestigationofclinicalmycobacteriumtuberculosisisolatesinkorea AT changchulhunl comparisonofanautomatedrepetitivesequencebasedpcrmicrobialtypingsystemwithis6110restrictionfragmentlengthpolymorphismforepidemiologicinvestigationofclinicalmycobacteriumtuberculosisisolatesinkorea AT kimjeongman comparisonofanautomatedrepetitivesequencebasedpcrmicrobialtypingsystemwithis6110restrictionfragmentlengthpolymorphismforepidemiologicinvestigationofclinicalmycobacteriumtuberculosisisolatesinkorea |