Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea

Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR (rep-PCR)-based DiversiLab™ system (bioMérieux, France) over the IS6110...

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Autores principales: Jang, Mi Hee, Choi, Go Eun, Shin, Bo-Moon, Lee, Seon Ho, Kim, Sung-Ryul, Chang, Chulhun L., Kim, Jeong-Man
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Laboratory Medicine 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3190008/
https://www.ncbi.nlm.nih.gov/pubmed/22016683
http://dx.doi.org/10.3343/kjlm.2011.31.4.282
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author Jang, Mi Hee
Choi, Go Eun
Shin, Bo-Moon
Lee, Seon Ho
Kim, Sung-Ryul
Chang, Chulhun L.
Kim, Jeong-Man
author_facet Jang, Mi Hee
Choi, Go Eun
Shin, Bo-Moon
Lee, Seon Ho
Kim, Sung-Ryul
Chang, Chulhun L.
Kim, Jeong-Man
author_sort Jang, Mi Hee
collection PubMed
description Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR (rep-PCR)-based DiversiLab™ system (bioMérieux, France) over the IS6110-restriction fragment length polymorphism analysis for detecting Mycobacterium tuberculosis. In all, 89 clinical M. tuberculosis isolates collected nationwide from Korea were used. The DiversiLab system allocated the 89 isolates to 8 groups with 1 unique isolate when a similarity level of 95% was applied. Seventy-six isolates of the Beijing family and 13 isolates of non-Beijing family strains were irregularly distributed regardless of rep-PCR groups. The DiversiLab system generated a rapid, sensitive, and standardized result. It can be used to conduct molecular epidemiologic studies to identify clinical M. tuberculosis isolates in Korea.
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spelling pubmed-31900082011-10-20 Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea Jang, Mi Hee Choi, Go Eun Shin, Bo-Moon Lee, Seon Ho Kim, Sung-Ryul Chang, Chulhun L. Kim, Jeong-Man Korean J Lab Med Brief Communication Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR (rep-PCR)-based DiversiLab™ system (bioMérieux, France) over the IS6110-restriction fragment length polymorphism analysis for detecting Mycobacterium tuberculosis. In all, 89 clinical M. tuberculosis isolates collected nationwide from Korea were used. The DiversiLab system allocated the 89 isolates to 8 groups with 1 unique isolate when a similarity level of 95% was applied. Seventy-six isolates of the Beijing family and 13 isolates of non-Beijing family strains were irregularly distributed regardless of rep-PCR groups. The DiversiLab system generated a rapid, sensitive, and standardized result. It can be used to conduct molecular epidemiologic studies to identify clinical M. tuberculosis isolates in Korea. The Korean Society for Laboratory Medicine 2011-10 2011-10-03 /pmc/articles/PMC3190008/ /pubmed/22016683 http://dx.doi.org/10.3343/kjlm.2011.31.4.282 Text en Copyright © 2011 The Korean Society for Laboratory Medicine http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
Jang, Mi Hee
Choi, Go Eun
Shin, Bo-Moon
Lee, Seon Ho
Kim, Sung-Ryul
Chang, Chulhun L.
Kim, Jeong-Man
Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea
title Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea
title_full Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea
title_fullStr Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea
title_full_unstemmed Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea
title_short Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea
title_sort comparison of an automated repetitive sequence-based pcr microbial typing system with is6110-restriction fragment length polymorphism for epidemiologic investigation of clinical mycobacterium tuberculosis isolates in korea
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3190008/
https://www.ncbi.nlm.nih.gov/pubmed/22016683
http://dx.doi.org/10.3343/kjlm.2011.31.4.282
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