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Identical Functional Organization of Nonpolytene and Polytene Chromosomes in Drosophila melanogaster

Salivary gland polytene chromosomes demonstrate banding pattern, genetic meaning of which is an enigma for decades. Till now it is not known how to mark the band/interband borders on physical map of DNA and structures of polytene chromosomes are not characterized in molecular and genetic terms. It i...

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Autores principales: Vatolina, Tatyana Yu., Boldyreva, Lidiya V., Demakova, Olga V., Demakov, Sergey A., Kokoza, Elena B., Semeshin, Valeriy F., Babenko, Vladimir N., Goncharov, Fedor P., Belyaeva, Elena S., Zhimulev, Igor F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3191165/
https://www.ncbi.nlm.nih.gov/pubmed/22022482
http://dx.doi.org/10.1371/journal.pone.0025960
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author Vatolina, Tatyana Yu.
Boldyreva, Lidiya V.
Demakova, Olga V.
Demakov, Sergey A.
Kokoza, Elena B.
Semeshin, Valeriy F.
Babenko, Vladimir N.
Goncharov, Fedor P.
Belyaeva, Elena S.
Zhimulev, Igor F.
author_facet Vatolina, Tatyana Yu.
Boldyreva, Lidiya V.
Demakova, Olga V.
Demakov, Sergey A.
Kokoza, Elena B.
Semeshin, Valeriy F.
Babenko, Vladimir N.
Goncharov, Fedor P.
Belyaeva, Elena S.
Zhimulev, Igor F.
author_sort Vatolina, Tatyana Yu.
collection PubMed
description Salivary gland polytene chromosomes demonstrate banding pattern, genetic meaning of which is an enigma for decades. Till now it is not known how to mark the band/interband borders on physical map of DNA and structures of polytene chromosomes are not characterized in molecular and genetic terms. It is not known either similar banding pattern exists in chromosomes of regular diploid mitotically dividing nonpolytene cells. Using the newly developed approach permitting to identify the interband material and localization data of interband-specific proteins from modENCODE and other genome-wide projects, we identify physical limits of bands and interbands in small cytological region 9F13-10B3 of the X chromosome in D. melanogaster, as well as characterize their general molecular features. Our results suggests that the polytene and interphase cell line chromosomes have practically the same patterns of bands and interbands reflecting, probably, the basic principle of interphase chromosome organization. Two types of bands have been described in chromosomes, early and late-replicating, which differ in many aspects of their protein and genetic content. As appeared, origin recognition complexes are located almost totally in the interbands of chromosomes.
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spelling pubmed-31911652011-10-21 Identical Functional Organization of Nonpolytene and Polytene Chromosomes in Drosophila melanogaster Vatolina, Tatyana Yu. Boldyreva, Lidiya V. Demakova, Olga V. Demakov, Sergey A. Kokoza, Elena B. Semeshin, Valeriy F. Babenko, Vladimir N. Goncharov, Fedor P. Belyaeva, Elena S. Zhimulev, Igor F. PLoS One Research Article Salivary gland polytene chromosomes demonstrate banding pattern, genetic meaning of which is an enigma for decades. Till now it is not known how to mark the band/interband borders on physical map of DNA and structures of polytene chromosomes are not characterized in molecular and genetic terms. It is not known either similar banding pattern exists in chromosomes of regular diploid mitotically dividing nonpolytene cells. Using the newly developed approach permitting to identify the interband material and localization data of interband-specific proteins from modENCODE and other genome-wide projects, we identify physical limits of bands and interbands in small cytological region 9F13-10B3 of the X chromosome in D. melanogaster, as well as characterize their general molecular features. Our results suggests that the polytene and interphase cell line chromosomes have practically the same patterns of bands and interbands reflecting, probably, the basic principle of interphase chromosome organization. Two types of bands have been described in chromosomes, early and late-replicating, which differ in many aspects of their protein and genetic content. As appeared, origin recognition complexes are located almost totally in the interbands of chromosomes. Public Library of Science 2011-10-11 /pmc/articles/PMC3191165/ /pubmed/22022482 http://dx.doi.org/10.1371/journal.pone.0025960 Text en Vatolina et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Vatolina, Tatyana Yu.
Boldyreva, Lidiya V.
Demakova, Olga V.
Demakov, Sergey A.
Kokoza, Elena B.
Semeshin, Valeriy F.
Babenko, Vladimir N.
Goncharov, Fedor P.
Belyaeva, Elena S.
Zhimulev, Igor F.
Identical Functional Organization of Nonpolytene and Polytene Chromosomes in Drosophila melanogaster
title Identical Functional Organization of Nonpolytene and Polytene Chromosomes in Drosophila melanogaster
title_full Identical Functional Organization of Nonpolytene and Polytene Chromosomes in Drosophila melanogaster
title_fullStr Identical Functional Organization of Nonpolytene and Polytene Chromosomes in Drosophila melanogaster
title_full_unstemmed Identical Functional Organization of Nonpolytene and Polytene Chromosomes in Drosophila melanogaster
title_short Identical Functional Organization of Nonpolytene and Polytene Chromosomes in Drosophila melanogaster
title_sort identical functional organization of nonpolytene and polytene chromosomes in drosophila melanogaster
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3191165/
https://www.ncbi.nlm.nih.gov/pubmed/22022482
http://dx.doi.org/10.1371/journal.pone.0025960
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