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Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalis from Thai Isolates: evidence of genetic exchange or Mixed Infections?

BACKGROUND: The glutamate dehydrogenase gene (gdh) is one of the most popular and useful genetic markers for the genotypic analysis of Giardia duodenalis (syn. G. lamblia, G. intestinalis), the protozoan that widely causes enteric disease in humans. To determine the distribution of genotypes of G. d...

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Autores principales: Siripattanapipong, Suradej, Leelayoova, Saovanee, Mungthin, Mathirut, Thompson, RC Andrew, Boontanom, Parima, Saksirisampant, Wilai, Tan-ariya, Peerapan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3191338/
https://www.ncbi.nlm.nih.gov/pubmed/21933419
http://dx.doi.org/10.1186/1471-2180-11-206
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author Siripattanapipong, Suradej
Leelayoova, Saovanee
Mungthin, Mathirut
Thompson, RC Andrew
Boontanom, Parima
Saksirisampant, Wilai
Tan-ariya, Peerapan
author_facet Siripattanapipong, Suradej
Leelayoova, Saovanee
Mungthin, Mathirut
Thompson, RC Andrew
Boontanom, Parima
Saksirisampant, Wilai
Tan-ariya, Peerapan
author_sort Siripattanapipong, Suradej
collection PubMed
description BACKGROUND: The glutamate dehydrogenase gene (gdh) is one of the most popular and useful genetic markers for the genotypic analysis of Giardia duodenalis (syn. G. lamblia, G. intestinalis), the protozoan that widely causes enteric disease in humans. To determine the distribution of genotypes of G. duodenalis in Thai populations and to investigate the extent of sequence variation at this locus, 42 fecal samples were collected from 3 regions of Thailand i.e., Central, Northern, and Eastern regions. All specimens were analyzed using PCR-based genotyping and recombinant subcloning methods. RESULTS: The results showed that the prevalence of assemblages A and B among these populations was approximately equal, 20 (47.6%) and 22 (52.4%), respectively. Sequence analysis revealed that the nucleotide diversity of assemblage B was significantly greater than that in assemblage A. Among all assemblage B positive specimens, the allelic sequence divergence within isolates was detected. Nine isolates showed mixed alleles, ranged from three to nine distinct alleles per isolate. Statistical analysis demonstrated the occurrence of genetic recombination within subassemblages BIII and BIV was likely. CONCLUSION: This study supports increasing evidence that G. duodenalis has the potential for genetic exchange.
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spelling pubmed-31913382011-10-13 Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalis from Thai Isolates: evidence of genetic exchange or Mixed Infections? Siripattanapipong, Suradej Leelayoova, Saovanee Mungthin, Mathirut Thompson, RC Andrew Boontanom, Parima Saksirisampant, Wilai Tan-ariya, Peerapan BMC Microbiol Research Article BACKGROUND: The glutamate dehydrogenase gene (gdh) is one of the most popular and useful genetic markers for the genotypic analysis of Giardia duodenalis (syn. G. lamblia, G. intestinalis), the protozoan that widely causes enteric disease in humans. To determine the distribution of genotypes of G. duodenalis in Thai populations and to investigate the extent of sequence variation at this locus, 42 fecal samples were collected from 3 regions of Thailand i.e., Central, Northern, and Eastern regions. All specimens were analyzed using PCR-based genotyping and recombinant subcloning methods. RESULTS: The results showed that the prevalence of assemblages A and B among these populations was approximately equal, 20 (47.6%) and 22 (52.4%), respectively. Sequence analysis revealed that the nucleotide diversity of assemblage B was significantly greater than that in assemblage A. Among all assemblage B positive specimens, the allelic sequence divergence within isolates was detected. Nine isolates showed mixed alleles, ranged from three to nine distinct alleles per isolate. Statistical analysis demonstrated the occurrence of genetic recombination within subassemblages BIII and BIV was likely. CONCLUSION: This study supports increasing evidence that G. duodenalis has the potential for genetic exchange. BioMed Central 2011-09-20 /pmc/articles/PMC3191338/ /pubmed/21933419 http://dx.doi.org/10.1186/1471-2180-11-206 Text en Copyright ©2011 Siripattanapipong et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Siripattanapipong, Suradej
Leelayoova, Saovanee
Mungthin, Mathirut
Thompson, RC Andrew
Boontanom, Parima
Saksirisampant, Wilai
Tan-ariya, Peerapan
Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalis from Thai Isolates: evidence of genetic exchange or Mixed Infections?
title Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalis from Thai Isolates: evidence of genetic exchange or Mixed Infections?
title_full Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalis from Thai Isolates: evidence of genetic exchange or Mixed Infections?
title_fullStr Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalis from Thai Isolates: evidence of genetic exchange or Mixed Infections?
title_full_unstemmed Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalis from Thai Isolates: evidence of genetic exchange or Mixed Infections?
title_short Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalis from Thai Isolates: evidence of genetic exchange or Mixed Infections?
title_sort clonal diversity of the glutamate dehydrogenase gene in giardia duodenalis from thai isolates: evidence of genetic exchange or mixed infections?
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3191338/
https://www.ncbi.nlm.nih.gov/pubmed/21933419
http://dx.doi.org/10.1186/1471-2180-11-206
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