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Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger
A fungal isolate with capability to grow in keratinous substrate as only source of carbon and nitrogen was identified as Aspergillus niger using the sequencing of the ITS region of the rDNA. This strain produced a slightly acid keratinase and an acid protease during cultivation in feather meal. The...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE-Hindawi Access to Research
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3191812/ https://www.ncbi.nlm.nih.gov/pubmed/22007293 http://dx.doi.org/10.4061/2011/487093 |
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author | Lopes, Fernanda Cortez Silva, Lucas André Dedavid e Tichota, Deise Michele Daroit, Daniel Joner Velho, Renata Voltolini Pereira, Jamile Queiroz Corrêa, Ana Paula Folmer Brandelli, Adriano |
author_facet | Lopes, Fernanda Cortez Silva, Lucas André Dedavid e Tichota, Deise Michele Daroit, Daniel Joner Velho, Renata Voltolini Pereira, Jamile Queiroz Corrêa, Ana Paula Folmer Brandelli, Adriano |
author_sort | Lopes, Fernanda Cortez |
collection | PubMed |
description | A fungal isolate with capability to grow in keratinous substrate as only source of carbon and nitrogen was identified as Aspergillus niger using the sequencing of the ITS region of the rDNA. This strain produced a slightly acid keratinase and an acid protease during cultivation in feather meal. The peak of keratinolytic activity occurred in 48 h and the maximum proteolytic activity in 96 h. These enzymes were partly characterized as serine protease and aspartic protease, respectively. The effects of feather meal concentration and initial pH on enzyme production were evaluated using a central composite design combined with response surface methodology. The optimal conditions were determined as pH 5.0 for protease and 7.8 for keratinase and 20 g/L of feather meal, showing that both models were predictive. Production of keratinases by A. niger is a less-exploited field that might represent a novel and promising biotechnological application for this microorganism. |
format | Online Article Text |
id | pubmed-3191812 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | SAGE-Hindawi Access to Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-31918122011-10-17 Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger Lopes, Fernanda Cortez Silva, Lucas André Dedavid e Tichota, Deise Michele Daroit, Daniel Joner Velho, Renata Voltolini Pereira, Jamile Queiroz Corrêa, Ana Paula Folmer Brandelli, Adriano Enzyme Res Research Article A fungal isolate with capability to grow in keratinous substrate as only source of carbon and nitrogen was identified as Aspergillus niger using the sequencing of the ITS region of the rDNA. This strain produced a slightly acid keratinase and an acid protease during cultivation in feather meal. The peak of keratinolytic activity occurred in 48 h and the maximum proteolytic activity in 96 h. These enzymes were partly characterized as serine protease and aspartic protease, respectively. The effects of feather meal concentration and initial pH on enzyme production were evaluated using a central composite design combined with response surface methodology. The optimal conditions were determined as pH 5.0 for protease and 7.8 for keratinase and 20 g/L of feather meal, showing that both models were predictive. Production of keratinases by A. niger is a less-exploited field that might represent a novel and promising biotechnological application for this microorganism. SAGE-Hindawi Access to Research 2011 2011-10-10 /pmc/articles/PMC3191812/ /pubmed/22007293 http://dx.doi.org/10.4061/2011/487093 Text en Copyright © 2011 Fernanda Cortez Lopes et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lopes, Fernanda Cortez Silva, Lucas André Dedavid e Tichota, Deise Michele Daroit, Daniel Joner Velho, Renata Voltolini Pereira, Jamile Queiroz Corrêa, Ana Paula Folmer Brandelli, Adriano Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger |
title | Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger
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title_full | Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger
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title_fullStr | Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger
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title_full_unstemmed | Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger
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title_short | Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger
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title_sort | production of proteolytic enzymes by a keratin-degrading aspergillus niger |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3191812/ https://www.ncbi.nlm.nih.gov/pubmed/22007293 http://dx.doi.org/10.4061/2011/487093 |
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