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An efficient multiplex genotyping approach for detecting the major worldwide human Y-chromosome haplogroups

The Y chromosome is paternally inherited and therefore serves as an evolutionary marker of patrilineal descent. Worldwide DNA variation within the non-recombining portion of the Y chromosome can be represented as a monophyletic phylogenetic tree in which the branches (haplogroups) are defined by at...

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Autores principales: van Oven, Mannis, Ralf, Arwin, Kayser, Manfred
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3192277/
https://www.ncbi.nlm.nih.gov/pubmed/21785904
http://dx.doi.org/10.1007/s00414-011-0605-2
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author van Oven, Mannis
Ralf, Arwin
Kayser, Manfred
author_facet van Oven, Mannis
Ralf, Arwin
Kayser, Manfred
author_sort van Oven, Mannis
collection PubMed
description The Y chromosome is paternally inherited and therefore serves as an evolutionary marker of patrilineal descent. Worldwide DNA variation within the non-recombining portion of the Y chromosome can be represented as a monophyletic phylogenetic tree in which the branches (haplogroups) are defined by at least one SNP. Previous human population genetics research has produced a wealth of knowledge about the worldwide distribution of Y-SNP haplogroups. Here, we apply previous and very recent knowledge on the Y-SNP phylogeny and Y-haplogroup distribution by introducing two multiplex genotyping assays that allow for the hierarchical detection of 28 Y-SNPs defining the major worldwide Y haplogroups. PCR amplicons were kept small to make the method sensitive and thereby applicable to DNA of limited amount and/or quality such as in forensic settings. These Y-SNP assays thus form a valuable tool for researchers in the fields of forensic genetics and genetic anthropology to infer a man's patrilineal bio-geographic ancestry from DNA.
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spelling pubmed-31922772011-10-24 An efficient multiplex genotyping approach for detecting the major worldwide human Y-chromosome haplogroups van Oven, Mannis Ralf, Arwin Kayser, Manfred Int J Legal Med Technical Note The Y chromosome is paternally inherited and therefore serves as an evolutionary marker of patrilineal descent. Worldwide DNA variation within the non-recombining portion of the Y chromosome can be represented as a monophyletic phylogenetic tree in which the branches (haplogroups) are defined by at least one SNP. Previous human population genetics research has produced a wealth of knowledge about the worldwide distribution of Y-SNP haplogroups. Here, we apply previous and very recent knowledge on the Y-SNP phylogeny and Y-haplogroup distribution by introducing two multiplex genotyping assays that allow for the hierarchical detection of 28 Y-SNPs defining the major worldwide Y haplogroups. PCR amplicons were kept small to make the method sensitive and thereby applicable to DNA of limited amount and/or quality such as in forensic settings. These Y-SNP assays thus form a valuable tool for researchers in the fields of forensic genetics and genetic anthropology to infer a man's patrilineal bio-geographic ancestry from DNA. Springer-Verlag 2011-07-22 2011 /pmc/articles/PMC3192277/ /pubmed/21785904 http://dx.doi.org/10.1007/s00414-011-0605-2 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Technical Note
van Oven, Mannis
Ralf, Arwin
Kayser, Manfred
An efficient multiplex genotyping approach for detecting the major worldwide human Y-chromosome haplogroups
title An efficient multiplex genotyping approach for detecting the major worldwide human Y-chromosome haplogroups
title_full An efficient multiplex genotyping approach for detecting the major worldwide human Y-chromosome haplogroups
title_fullStr An efficient multiplex genotyping approach for detecting the major worldwide human Y-chromosome haplogroups
title_full_unstemmed An efficient multiplex genotyping approach for detecting the major worldwide human Y-chromosome haplogroups
title_short An efficient multiplex genotyping approach for detecting the major worldwide human Y-chromosome haplogroups
title_sort efficient multiplex genotyping approach for detecting the major worldwide human y-chromosome haplogroups
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3192277/
https://www.ncbi.nlm.nih.gov/pubmed/21785904
http://dx.doi.org/10.1007/s00414-011-0605-2
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