Cargando…
Pulsed electromagnetic fields stimulation prevents steroid-induced osteonecrosis in rats
BACKGROUND: Pulsed electromagnetic fields (PEMF) stimulation has been used successfully to treat nonunion fractures and femoral head osteonecrosis, but relatively little is known about its effects on preventing steroid-induced osteonecrosis. The purpose of the study was to investigate the effects of...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3192716/ https://www.ncbi.nlm.nih.gov/pubmed/21958301 http://dx.doi.org/10.1186/1471-2474-12-215 |
_version_ | 1782213779107348480 |
---|---|
author | Ding, Shuai Peng, Hao Fang, Hong-Song Zhou, Jian-Lin Wang, Zhe |
author_facet | Ding, Shuai Peng, Hao Fang, Hong-Song Zhou, Jian-Lin Wang, Zhe |
author_sort | Ding, Shuai |
collection | PubMed |
description | BACKGROUND: Pulsed electromagnetic fields (PEMF) stimulation has been used successfully to treat nonunion fractures and femoral head osteonecrosis, but relatively little is known about its effects on preventing steroid-induced osteonecrosis. The purpose of the study was to investigate the effects of PEMF stimulation on the prevention of steroid-induced osteonecrosis in rats and explore the underlying mechanisms. METHODS: Seventy-two male adult Wistar rats were divided into three groups and treated as follows. (1) PEMF stimulation group (PEMF group, n = 24): intravenously injected with lipopolysaccharide (LPS, 10 μg/kg) on day 0 and intramuscularly injected with methylprednisolone acetate (MPSL, 20 mg/kg) on days 1, 2 and 3, then subjected to PEMF stimulation 4 h per day for 1 to 8 weeks. (2) Methylprednisolone-treated group (MPSL group, n = 24): injected the same dose of LPS and MPSL as the PEMF group but without exposure to PEMF. (3) Control group (PS group, n = 24): injected 0.9% saline in the same mode at the same time points. The incidence of osteonecrosis, serum lipid levels and the mRNA and protein expression of transforming growth factor β1 (TGF-β1) in the proximal femur were measured 1, 2, 4 and 8 weeks after the last MPSL (or saline) injection. RESULTS: The incidence of osteonecrosis in the PEMF group (29%) was significantly lower than that observed in the MPSL group (75%), while no osteonecrosis was observed in the PS group. The serum lipid levels were significantly lower in the PEMF and PS groups than in the MPSL group. Compared with the MPSL and PS groups, the mRNA expression of TGF-β1 increased, reaching a peak 1 week after PEMF treatment, and remained high for 4 weeks, then declined at 8 weeks, whereas the protein expression of TGF-β1 increased, reaching a peak at 2 weeks after PEMF treatment, and remained high for 8 weeks. CONCLUSIONS: PEMF stimulation can prevent steroid-induced osteonecrosis in rats, and the underlying mechanisms involve decreased serum lipid levels and increased expression of TGF-β1. |
format | Online Article Text |
id | pubmed-3192716 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31927162011-10-14 Pulsed electromagnetic fields stimulation prevents steroid-induced osteonecrosis in rats Ding, Shuai Peng, Hao Fang, Hong-Song Zhou, Jian-Lin Wang, Zhe BMC Musculoskelet Disord Research Article BACKGROUND: Pulsed electromagnetic fields (PEMF) stimulation has been used successfully to treat nonunion fractures and femoral head osteonecrosis, but relatively little is known about its effects on preventing steroid-induced osteonecrosis. The purpose of the study was to investigate the effects of PEMF stimulation on the prevention of steroid-induced osteonecrosis in rats and explore the underlying mechanisms. METHODS: Seventy-two male adult Wistar rats were divided into three groups and treated as follows. (1) PEMF stimulation group (PEMF group, n = 24): intravenously injected with lipopolysaccharide (LPS, 10 μg/kg) on day 0 and intramuscularly injected with methylprednisolone acetate (MPSL, 20 mg/kg) on days 1, 2 and 3, then subjected to PEMF stimulation 4 h per day for 1 to 8 weeks. (2) Methylprednisolone-treated group (MPSL group, n = 24): injected the same dose of LPS and MPSL as the PEMF group but without exposure to PEMF. (3) Control group (PS group, n = 24): injected 0.9% saline in the same mode at the same time points. The incidence of osteonecrosis, serum lipid levels and the mRNA and protein expression of transforming growth factor β1 (TGF-β1) in the proximal femur were measured 1, 2, 4 and 8 weeks after the last MPSL (or saline) injection. RESULTS: The incidence of osteonecrosis in the PEMF group (29%) was significantly lower than that observed in the MPSL group (75%), while no osteonecrosis was observed in the PS group. The serum lipid levels were significantly lower in the PEMF and PS groups than in the MPSL group. Compared with the MPSL and PS groups, the mRNA expression of TGF-β1 increased, reaching a peak 1 week after PEMF treatment, and remained high for 4 weeks, then declined at 8 weeks, whereas the protein expression of TGF-β1 increased, reaching a peak at 2 weeks after PEMF treatment, and remained high for 8 weeks. CONCLUSIONS: PEMF stimulation can prevent steroid-induced osteonecrosis in rats, and the underlying mechanisms involve decreased serum lipid levels and increased expression of TGF-β1. BioMed Central 2011-09-29 /pmc/articles/PMC3192716/ /pubmed/21958301 http://dx.doi.org/10.1186/1471-2474-12-215 Text en Copyright ©2011 Ding et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ding, Shuai Peng, Hao Fang, Hong-Song Zhou, Jian-Lin Wang, Zhe Pulsed electromagnetic fields stimulation prevents steroid-induced osteonecrosis in rats |
title | Pulsed electromagnetic fields stimulation prevents steroid-induced osteonecrosis in rats |
title_full | Pulsed electromagnetic fields stimulation prevents steroid-induced osteonecrosis in rats |
title_fullStr | Pulsed electromagnetic fields stimulation prevents steroid-induced osteonecrosis in rats |
title_full_unstemmed | Pulsed electromagnetic fields stimulation prevents steroid-induced osteonecrosis in rats |
title_short | Pulsed electromagnetic fields stimulation prevents steroid-induced osteonecrosis in rats |
title_sort | pulsed electromagnetic fields stimulation prevents steroid-induced osteonecrosis in rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3192716/ https://www.ncbi.nlm.nih.gov/pubmed/21958301 http://dx.doi.org/10.1186/1471-2474-12-215 |
work_keys_str_mv | AT dingshuai pulsedelectromagneticfieldsstimulationpreventssteroidinducedosteonecrosisinrats AT penghao pulsedelectromagneticfieldsstimulationpreventssteroidinducedosteonecrosisinrats AT fanghongsong pulsedelectromagneticfieldsstimulationpreventssteroidinducedosteonecrosisinrats AT zhoujianlin pulsedelectromagneticfieldsstimulationpreventssteroidinducedosteonecrosisinrats AT wangzhe pulsedelectromagneticfieldsstimulationpreventssteroidinducedosteonecrosisinrats |