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Spatial control of Cdc42 activation determines cell width in fission yeast

The fission yeast Schizosaccharomyces pombe is a rod-shaped cell that grows by linear extension at the cell tips, with a nearly constant width throughout the cell cycle. This simple geometry makes it an ideal system for studying the control of cellular dimensions. In this study, we carried out a nea...

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Autores principales: Kelly, Felice D., Nurse, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3192860/
https://www.ncbi.nlm.nih.gov/pubmed/21849474
http://dx.doi.org/10.1091/mbc.E11-01-0057
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author Kelly, Felice D.
Nurse, Paul
author_facet Kelly, Felice D.
Nurse, Paul
author_sort Kelly, Felice D.
collection PubMed
description The fission yeast Schizosaccharomyces pombe is a rod-shaped cell that grows by linear extension at the cell tips, with a nearly constant width throughout the cell cycle. This simple geometry makes it an ideal system for studying the control of cellular dimensions. In this study, we carried out a near-genome-wide screen for mutants wider than wild-type cells. We found 11 deletion mutants that were wider; seven of the deleted genes are implicated in the control of the small GTPase Cdc42, including the Cdc42 guanine nucleotide exchange factor (GEF) Scd1 and the Cdc42 GTPase-activating protein (GAP) Rga4. Deletions of rga4 and scd1 had additive effects on cell width, and the proteins localized independently of one another, with Rga4 located at the cell sides and Scd1 at the cell tips. Activated Cdc42 localization is altered in rga4Δ, scd1Δ, and scd2Δ mutants. Delocalization and ectopic retargeting experiments showed that the localizations of Rga4 and Scd1 are crucial for their roles in determining cell width. We propose that the GAP Rga4 and the GEF Scd1 establish a gradient of activated Cdc42 within the cellular tip plasma membrane, and it is this gradient that determines cell growth-zone size and normal cell width.
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spelling pubmed-31928602011-12-30 Spatial control of Cdc42 activation determines cell width in fission yeast Kelly, Felice D. Nurse, Paul Mol Biol Cell Articles The fission yeast Schizosaccharomyces pombe is a rod-shaped cell that grows by linear extension at the cell tips, with a nearly constant width throughout the cell cycle. This simple geometry makes it an ideal system for studying the control of cellular dimensions. In this study, we carried out a near-genome-wide screen for mutants wider than wild-type cells. We found 11 deletion mutants that were wider; seven of the deleted genes are implicated in the control of the small GTPase Cdc42, including the Cdc42 guanine nucleotide exchange factor (GEF) Scd1 and the Cdc42 GTPase-activating protein (GAP) Rga4. Deletions of rga4 and scd1 had additive effects on cell width, and the proteins localized independently of one another, with Rga4 located at the cell sides and Scd1 at the cell tips. Activated Cdc42 localization is altered in rga4Δ, scd1Δ, and scd2Δ mutants. Delocalization and ectopic retargeting experiments showed that the localizations of Rga4 and Scd1 are crucial for their roles in determining cell width. We propose that the GAP Rga4 and the GEF Scd1 establish a gradient of activated Cdc42 within the cellular tip plasma membrane, and it is this gradient that determines cell growth-zone size and normal cell width. The American Society for Cell Biology 2011-10-15 /pmc/articles/PMC3192860/ /pubmed/21849474 http://dx.doi.org/10.1091/mbc.E11-01-0057 Text en © 2011 Kelly and Nurse. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Kelly, Felice D.
Nurse, Paul
Spatial control of Cdc42 activation determines cell width in fission yeast
title Spatial control of Cdc42 activation determines cell width in fission yeast
title_full Spatial control of Cdc42 activation determines cell width in fission yeast
title_fullStr Spatial control of Cdc42 activation determines cell width in fission yeast
title_full_unstemmed Spatial control of Cdc42 activation determines cell width in fission yeast
title_short Spatial control of Cdc42 activation determines cell width in fission yeast
title_sort spatial control of cdc42 activation determines cell width in fission yeast
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3192860/
https://www.ncbi.nlm.nih.gov/pubmed/21849474
http://dx.doi.org/10.1091/mbc.E11-01-0057
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