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DNA alteration and programmed cell death during ageing of sunflower seed
Sunflower (Helianthus annuus L.) seed viability is affected by moisture content (MC) during ageing and is related to accumulation of hydrogen peroxide and changes in energy metabolism. The aim of the present work was to investigate the effect of ageing on DNA alteration events by RAPD (random amplif...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3193007/ https://www.ncbi.nlm.nih.gov/pubmed/21765164 http://dx.doi.org/10.1093/jxb/err198 |
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author | El-Maarouf-Bouteau, Hayat Mazuy, Claire Corbineau, Françoise Bailly, Christophe |
author_facet | El-Maarouf-Bouteau, Hayat Mazuy, Claire Corbineau, Françoise Bailly, Christophe |
author_sort | El-Maarouf-Bouteau, Hayat |
collection | PubMed |
description | Sunflower (Helianthus annuus L.) seed viability is affected by moisture content (MC) during ageing and is related to accumulation of hydrogen peroxide and changes in energy metabolism. The aim of the present work was to investigate the effect of ageing on DNA alteration events by RAPD (random amplification of polymorphic DNA) analysis and to determine whether loss of seed viability might correspond to a controlled programmed cell death (PCD). Ageing of sunflower seeds was carried out at 35 °C for 7 d at different MCs. The higher the MC, the lower was the seed viability. RAPD analysis showed that DNA alterations occurred during ageing especially in seeds containing a high MC. In addition, PCD, as revealed by DNA fragmentation and TUNEL (terminal deoxynucleotide transferase-mediated dUTP nick-end labelling) assay, was detected in aged seeds at MCs which resulted in ∼50% seed viability. At the cellular level, TUNEL assay and propidium iodide staining showed that cell death concerns all the cells of the embryonic axis. The quantification of the adenylate pool highlights mitochondrial dysfunction in aged seeds containing a high MC. The involvement of oxidative burst, mitochondria dysfunction, and PCD in seed loss of viability is proposed. |
format | Online Article Text |
id | pubmed-3193007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-31930072011-10-17 DNA alteration and programmed cell death during ageing of sunflower seed El-Maarouf-Bouteau, Hayat Mazuy, Claire Corbineau, Françoise Bailly, Christophe J Exp Bot Research Papers Sunflower (Helianthus annuus L.) seed viability is affected by moisture content (MC) during ageing and is related to accumulation of hydrogen peroxide and changes in energy metabolism. The aim of the present work was to investigate the effect of ageing on DNA alteration events by RAPD (random amplification of polymorphic DNA) analysis and to determine whether loss of seed viability might correspond to a controlled programmed cell death (PCD). Ageing of sunflower seeds was carried out at 35 °C for 7 d at different MCs. The higher the MC, the lower was the seed viability. RAPD analysis showed that DNA alterations occurred during ageing especially in seeds containing a high MC. In addition, PCD, as revealed by DNA fragmentation and TUNEL (terminal deoxynucleotide transferase-mediated dUTP nick-end labelling) assay, was detected in aged seeds at MCs which resulted in ∼50% seed viability. At the cellular level, TUNEL assay and propidium iodide staining showed that cell death concerns all the cells of the embryonic axis. The quantification of the adenylate pool highlights mitochondrial dysfunction in aged seeds containing a high MC. The involvement of oxidative burst, mitochondria dysfunction, and PCD in seed loss of viability is proposed. Oxford University Press 2011-10 2011-07-15 /pmc/articles/PMC3193007/ /pubmed/21765164 http://dx.doi.org/10.1093/jxb/err198 Text en © 2011 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details) |
spellingShingle | Research Papers El-Maarouf-Bouteau, Hayat Mazuy, Claire Corbineau, Françoise Bailly, Christophe DNA alteration and programmed cell death during ageing of sunflower seed |
title | DNA alteration and programmed cell death during ageing of sunflower seed |
title_full | DNA alteration and programmed cell death during ageing of sunflower seed |
title_fullStr | DNA alteration and programmed cell death during ageing of sunflower seed |
title_full_unstemmed | DNA alteration and programmed cell death during ageing of sunflower seed |
title_short | DNA alteration and programmed cell death during ageing of sunflower seed |
title_sort | dna alteration and programmed cell death during ageing of sunflower seed |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3193007/ https://www.ncbi.nlm.nih.gov/pubmed/21765164 http://dx.doi.org/10.1093/jxb/err198 |
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