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The expression and role of protein kinase C (PKC) epsilon in clear cell renal cell carcinoma

Protein kinase C epsilon (PKCε), an oncogene overexpressed in several human cancers, is involved in cell proliferation, migration, invasion, and survival. However, its roles in clear cell renal cell carcinoma (RCC) are unclear. This study aimed to investigate the functions of PKCε in RCC, especially...

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Autores principales: Huang, Bin, Cao, Kaiyuan, Li, Xiubo, Guo, Shengjie, Mao, Xiaopeng, Wang, Zhu, Zhuang, Jintao, Pan, Jincheng, Mo, Chengqiang, Chen, Junxing, Qiu, Shaopeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3193168/
https://www.ncbi.nlm.nih.gov/pubmed/21955404
http://dx.doi.org/10.1186/1756-9966-30-88
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author Huang, Bin
Cao, Kaiyuan
Li, Xiubo
Guo, Shengjie
Mao, Xiaopeng
Wang, Zhu
Zhuang, Jintao
Pan, Jincheng
Mo, Chengqiang
Chen, Junxing
Qiu, Shaopeng
author_facet Huang, Bin
Cao, Kaiyuan
Li, Xiubo
Guo, Shengjie
Mao, Xiaopeng
Wang, Zhu
Zhuang, Jintao
Pan, Jincheng
Mo, Chengqiang
Chen, Junxing
Qiu, Shaopeng
author_sort Huang, Bin
collection PubMed
description Protein kinase C epsilon (PKCε), an oncogene overexpressed in several human cancers, is involved in cell proliferation, migration, invasion, and survival. However, its roles in clear cell renal cell carcinoma (RCC) are unclear. This study aimed to investigate the functions of PKCε in RCC, especially in clear cell RCC, to determine the possibility of using it as a therapeutic target. By immunohistochemistry, we found that the expression of PKCε was up-regulated in RCCs and was associated with tumor Fuhrman grade and T stage in clear cell RCCs. Clone formation, wound healing, and Borden assays showed that down-regulating PKCε by RNA interference resulted in inhibition of the growth, migration, and invasion of clear cell RCC cell line 769P and, more importantly, sensitized cells to chemotherapeutic drugs as indicated by enhanced activity of caspase-3 in PKCε siRNA-transfected cells. These results indicate that the overexpression of PKCε is associated with an aggressive phenotype of clear cell RCC and may be a potential therapeutic target for this disease.
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spelling pubmed-31931682011-10-15 The expression and role of protein kinase C (PKC) epsilon in clear cell renal cell carcinoma Huang, Bin Cao, Kaiyuan Li, Xiubo Guo, Shengjie Mao, Xiaopeng Wang, Zhu Zhuang, Jintao Pan, Jincheng Mo, Chengqiang Chen, Junxing Qiu, Shaopeng J Exp Clin Cancer Res Research Protein kinase C epsilon (PKCε), an oncogene overexpressed in several human cancers, is involved in cell proliferation, migration, invasion, and survival. However, its roles in clear cell renal cell carcinoma (RCC) are unclear. This study aimed to investigate the functions of PKCε in RCC, especially in clear cell RCC, to determine the possibility of using it as a therapeutic target. By immunohistochemistry, we found that the expression of PKCε was up-regulated in RCCs and was associated with tumor Fuhrman grade and T stage in clear cell RCCs. Clone formation, wound healing, and Borden assays showed that down-regulating PKCε by RNA interference resulted in inhibition of the growth, migration, and invasion of clear cell RCC cell line 769P and, more importantly, sensitized cells to chemotherapeutic drugs as indicated by enhanced activity of caspase-3 in PKCε siRNA-transfected cells. These results indicate that the overexpression of PKCε is associated with an aggressive phenotype of clear cell RCC and may be a potential therapeutic target for this disease. BioMed Central 2011-09-28 /pmc/articles/PMC3193168/ /pubmed/21955404 http://dx.doi.org/10.1186/1756-9966-30-88 Text en Copyright ©2011 Huang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Huang, Bin
Cao, Kaiyuan
Li, Xiubo
Guo, Shengjie
Mao, Xiaopeng
Wang, Zhu
Zhuang, Jintao
Pan, Jincheng
Mo, Chengqiang
Chen, Junxing
Qiu, Shaopeng
The expression and role of protein kinase C (PKC) epsilon in clear cell renal cell carcinoma
title The expression and role of protein kinase C (PKC) epsilon in clear cell renal cell carcinoma
title_full The expression and role of protein kinase C (PKC) epsilon in clear cell renal cell carcinoma
title_fullStr The expression and role of protein kinase C (PKC) epsilon in clear cell renal cell carcinoma
title_full_unstemmed The expression and role of protein kinase C (PKC) epsilon in clear cell renal cell carcinoma
title_short The expression and role of protein kinase C (PKC) epsilon in clear cell renal cell carcinoma
title_sort expression and role of protein kinase c (pkc) epsilon in clear cell renal cell carcinoma
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3193168/
https://www.ncbi.nlm.nih.gov/pubmed/21955404
http://dx.doi.org/10.1186/1756-9966-30-88
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