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Cys-Ph-TAHA: a lanthanide binding tag for RDC and PCS enhanced protein NMR

Here we present Cys-Ph-TAHA, a new nonadentate lanthanide tag for the paramagnetic labelling of proteins. The tag can be easily synthesized and is stereochemically homogenous over a wide range of temperatures, yielding NMR spectra with a single set of peaks. Bound to ubiquitin, it induced large resi...

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Detalles Bibliográficos
Autores principales: Peters, Fabian, Maestre-Martinez, Mitcheell, Leonov, Andrei, Kovačič, Lidija, Becker, Stefan, Boelens, Rolf, Griesinger, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3193991/
https://www.ncbi.nlm.nih.gov/pubmed/21892794
http://dx.doi.org/10.1007/s10858-011-9560-y