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Cys-Ph-TAHA: a lanthanide binding tag for RDC and PCS enhanced protein NMR
Here we present Cys-Ph-TAHA, a new nonadentate lanthanide tag for the paramagnetic labelling of proteins. The tag can be easily synthesized and is stereochemically homogenous over a wide range of temperatures, yielding NMR spectra with a single set of peaks. Bound to ubiquitin, it induced large resi...
Autores principales: | Peters, Fabian, Maestre-Martinez, Mitcheell, Leonov, Andrei, Kovačič, Lidija, Becker, Stefan, Boelens, Rolf, Griesinger, Christian |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3193991/ https://www.ncbi.nlm.nih.gov/pubmed/21892794 http://dx.doi.org/10.1007/s10858-011-9560-y |
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