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A Human TREK-1/HEK Cell Line: A Highly Efficient Screening Tool for Drug Development in Neurological Diseases
TREK-1 potassium channels are involved in a number of physiopathological processes such as neuroprotection, pain and depression. Molecules able to open or to block these channels can be clinically important. Having a cell model for screening such molecules is of particular interest. Here, we describ...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3194802/ https://www.ncbi.nlm.nih.gov/pubmed/22022421 http://dx.doi.org/10.1371/journal.pone.0025602 |
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author | Moha ou Maati, Hamid Peyronnet, Rémi Devader, Christelle Veyssiere, Julie Labbal, Fabien Gandin, Carine Mazella, Jean Heurteaux, Catherine Borsotto, Marc |
author_facet | Moha ou Maati, Hamid Peyronnet, Rémi Devader, Christelle Veyssiere, Julie Labbal, Fabien Gandin, Carine Mazella, Jean Heurteaux, Catherine Borsotto, Marc |
author_sort | Moha ou Maati, Hamid |
collection | PubMed |
description | TREK-1 potassium channels are involved in a number of physiopathological processes such as neuroprotection, pain and depression. Molecules able to open or to block these channels can be clinically important. Having a cell model for screening such molecules is of particular interest. Here, we describe the development of the first available cell line that constituvely expresses the TREK-1 channel. The TREK-1 channel expressed by the h-TREK-1/HEK cell line has conserved all its modulation properties. It is opened by stretch, pH, polyunsaturated fatty acids and by the neuroprotective molecule, riluzole and it is blocked by spadin or fluoxetine. We also demonstrate that the h-TREK-1/HEK cell line is protected against ischemia by using the oxygen-glucose deprivation model. |
format | Online Article Text |
id | pubmed-3194802 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31948022011-10-21 A Human TREK-1/HEK Cell Line: A Highly Efficient Screening Tool for Drug Development in Neurological Diseases Moha ou Maati, Hamid Peyronnet, Rémi Devader, Christelle Veyssiere, Julie Labbal, Fabien Gandin, Carine Mazella, Jean Heurteaux, Catherine Borsotto, Marc PLoS One Research Article TREK-1 potassium channels are involved in a number of physiopathological processes such as neuroprotection, pain and depression. Molecules able to open or to block these channels can be clinically important. Having a cell model for screening such molecules is of particular interest. Here, we describe the development of the first available cell line that constituvely expresses the TREK-1 channel. The TREK-1 channel expressed by the h-TREK-1/HEK cell line has conserved all its modulation properties. It is opened by stretch, pH, polyunsaturated fatty acids and by the neuroprotective molecule, riluzole and it is blocked by spadin or fluoxetine. We also demonstrate that the h-TREK-1/HEK cell line is protected against ischemia by using the oxygen-glucose deprivation model. Public Library of Science 2011-10-14 /pmc/articles/PMC3194802/ /pubmed/22022421 http://dx.doi.org/10.1371/journal.pone.0025602 Text en Moha ou Maati et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Moha ou Maati, Hamid Peyronnet, Rémi Devader, Christelle Veyssiere, Julie Labbal, Fabien Gandin, Carine Mazella, Jean Heurteaux, Catherine Borsotto, Marc A Human TREK-1/HEK Cell Line: A Highly Efficient Screening Tool for Drug Development in Neurological Diseases |
title | A Human TREK-1/HEK Cell Line: A Highly Efficient Screening Tool for Drug Development in Neurological Diseases |
title_full | A Human TREK-1/HEK Cell Line: A Highly Efficient Screening Tool for Drug Development in Neurological Diseases |
title_fullStr | A Human TREK-1/HEK Cell Line: A Highly Efficient Screening Tool for Drug Development in Neurological Diseases |
title_full_unstemmed | A Human TREK-1/HEK Cell Line: A Highly Efficient Screening Tool for Drug Development in Neurological Diseases |
title_short | A Human TREK-1/HEK Cell Line: A Highly Efficient Screening Tool for Drug Development in Neurological Diseases |
title_sort | human trek-1/hek cell line: a highly efficient screening tool for drug development in neurological diseases |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3194802/ https://www.ncbi.nlm.nih.gov/pubmed/22022421 http://dx.doi.org/10.1371/journal.pone.0025602 |
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