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Organization and Molecular Evolution of CENP-A–Associated Satellite DNA Families in a Basal Primate Genome

Centromeric regions in many complex eukaryotic species contain highly repetitive satellite DNAs. Despite the diversity of centromeric DNA sequences among species, the functional centromeres in all species studied to date are marked by CENP-A, a centromere-specific histone H3 variant. Although it is...

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Autores principales: Lee, Hye-Ran, Hayden, Karen E., Willard, Huntington F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3194837/
https://www.ncbi.nlm.nih.gov/pubmed/21828373
http://dx.doi.org/10.1093/gbe/evr083
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author Lee, Hye-Ran
Hayden, Karen E.
Willard, Huntington F.
author_facet Lee, Hye-Ran
Hayden, Karen E.
Willard, Huntington F.
author_sort Lee, Hye-Ran
collection PubMed
description Centromeric regions in many complex eukaryotic species contain highly repetitive satellite DNAs. Despite the diversity of centromeric DNA sequences among species, the functional centromeres in all species studied to date are marked by CENP-A, a centromere-specific histone H3 variant. Although it is well established that families of multimeric higher-order alpha satellite are conserved at the centromeres of human and great ape chromosomes and that diverged monomeric alpha satellite is found in old and new world monkey genomes, little is known about the organization, function, and evolution of centromeric sequences in more distant primates, including lemurs. Aye-Aye (Daubentonia madagascariensis) is a basal primate and is located at a key position in the evolutionary tree to study centromeric satellite transitions in primate genomes. Using the approach of chromatin immunoprecipitation with antibodies directed to CENP-A, we have identified two satellite families, Daubentonia madagascariensis Aye-Aye 1 (DMA1) and Daubentonia madagascariensis Aye-Aye 2 (DMA2), related to each other but unrelated in sequence to alpha satellite or any other previously described primate or mammalian satellite DNA families. Here, we describe the initial genomic and phylogenetic organization of DMA1 and DMA2 and present evidence of higher-order repeats in Aye-Aye centromeric domains, providing an opportunity to study the emergence of chromosome-specific modes of satellite DNA evolution in primate genomes.
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spelling pubmed-31948372011-10-17 Organization and Molecular Evolution of CENP-A–Associated Satellite DNA Families in a Basal Primate Genome Lee, Hye-Ran Hayden, Karen E. Willard, Huntington F. Genome Biol Evol Research Articles Centromeric regions in many complex eukaryotic species contain highly repetitive satellite DNAs. Despite the diversity of centromeric DNA sequences among species, the functional centromeres in all species studied to date are marked by CENP-A, a centromere-specific histone H3 variant. Although it is well established that families of multimeric higher-order alpha satellite are conserved at the centromeres of human and great ape chromosomes and that diverged monomeric alpha satellite is found in old and new world monkey genomes, little is known about the organization, function, and evolution of centromeric sequences in more distant primates, including lemurs. Aye-Aye (Daubentonia madagascariensis) is a basal primate and is located at a key position in the evolutionary tree to study centromeric satellite transitions in primate genomes. Using the approach of chromatin immunoprecipitation with antibodies directed to CENP-A, we have identified two satellite families, Daubentonia madagascariensis Aye-Aye 1 (DMA1) and Daubentonia madagascariensis Aye-Aye 2 (DMA2), related to each other but unrelated in sequence to alpha satellite or any other previously described primate or mammalian satellite DNA families. Here, we describe the initial genomic and phylogenetic organization of DMA1 and DMA2 and present evidence of higher-order repeats in Aye-Aye centromeric domains, providing an opportunity to study the emergence of chromosome-specific modes of satellite DNA evolution in primate genomes. Oxford University Press 2011-08-09 /pmc/articles/PMC3194837/ /pubmed/21828373 http://dx.doi.org/10.1093/gbe/evr083 Text en The Author(s) 2011. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Lee, Hye-Ran
Hayden, Karen E.
Willard, Huntington F.
Organization and Molecular Evolution of CENP-A–Associated Satellite DNA Families in a Basal Primate Genome
title Organization and Molecular Evolution of CENP-A–Associated Satellite DNA Families in a Basal Primate Genome
title_full Organization and Molecular Evolution of CENP-A–Associated Satellite DNA Families in a Basal Primate Genome
title_fullStr Organization and Molecular Evolution of CENP-A–Associated Satellite DNA Families in a Basal Primate Genome
title_full_unstemmed Organization and Molecular Evolution of CENP-A–Associated Satellite DNA Families in a Basal Primate Genome
title_short Organization and Molecular Evolution of CENP-A–Associated Satellite DNA Families in a Basal Primate Genome
title_sort organization and molecular evolution of cenp-a–associated satellite dna families in a basal primate genome
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3194837/
https://www.ncbi.nlm.nih.gov/pubmed/21828373
http://dx.doi.org/10.1093/gbe/evr083
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