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The Coxiella burnetii Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation
Understanding the molecular pathogenesis of Coxiella burnetii, the causative agent of human Q fever, has historically been hindered by the technical difficulties of genetically manipulating obligate intracellular bacteria. The recent development of culture conditions suitable for axenic propagation...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Microbiology
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3195215/ https://www.ncbi.nlm.nih.gov/pubmed/22010216 http://dx.doi.org/10.1128/mBio.00226-11 |
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author | Newton, Hayley J. Roy, Craig R. |
author_facet | Newton, Hayley J. Roy, Craig R. |
author_sort | Newton, Hayley J. |
collection | PubMed |
description | Understanding the molecular pathogenesis of Coxiella burnetii, the causative agent of human Q fever, has historically been hindered by the technical difficulties of genetically manipulating obligate intracellular bacteria. The recent development of culture conditions suitable for axenic propagation of C. burnetii has paved the way for the application of a range of genetic techniques to address key questions within the field. Recent studies using mutational analysis have revealed that the C. burnetii Dot/Icm type 4 secretion system (T4SS) is an important virulence determinant that is essential for renovation of a lysosome into a mature Coxiella-containing vacuole (CCV) permissive of intracellular replication. Interestingly, a mutant of C. burnetii deficient in Dot/Icm function was found to be capable of replicating within the parasitophorous vacuole created by Leishmania amazonensis, which indicates that C. burnetii replication is not dependent on the cohort of Dot/Icm effector proteins per se but rather that the collective actions of effectors are required to create the specialized niche supportive of replication. Thus, a role for the Dot/Icm T4SS during the intracellular life cycle of C. burnetii has been more clearly defined by these studies, which demonstrate that advances in genetic analysis should allow future studies to focus on the intricacies of Dot/Icm effector functions that facilitate development of the unique CCV. |
format | Online Article Text |
id | pubmed-3195215 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | American Society of Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-31952152011-10-18 The Coxiella burnetii Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation Newton, Hayley J. Roy, Craig R. mBio Commentary Understanding the molecular pathogenesis of Coxiella burnetii, the causative agent of human Q fever, has historically been hindered by the technical difficulties of genetically manipulating obligate intracellular bacteria. The recent development of culture conditions suitable for axenic propagation of C. burnetii has paved the way for the application of a range of genetic techniques to address key questions within the field. Recent studies using mutational analysis have revealed that the C. burnetii Dot/Icm type 4 secretion system (T4SS) is an important virulence determinant that is essential for renovation of a lysosome into a mature Coxiella-containing vacuole (CCV) permissive of intracellular replication. Interestingly, a mutant of C. burnetii deficient in Dot/Icm function was found to be capable of replicating within the parasitophorous vacuole created by Leishmania amazonensis, which indicates that C. burnetii replication is not dependent on the cohort of Dot/Icm effector proteins per se but rather that the collective actions of effectors are required to create the specialized niche supportive of replication. Thus, a role for the Dot/Icm T4SS during the intracellular life cycle of C. burnetii has been more clearly defined by these studies, which demonstrate that advances in genetic analysis should allow future studies to focus on the intricacies of Dot/Icm effector functions that facilitate development of the unique CCV. American Society of Microbiology 2011-10-18 /pmc/articles/PMC3195215/ /pubmed/22010216 http://dx.doi.org/10.1128/mBio.00226-11 Text en Copyright © 2011 Newton and Roy http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Commentary Newton, Hayley J. Roy, Craig R. The Coxiella burnetii Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation |
title | The Coxiella burnetii Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation |
title_full | The Coxiella burnetii Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation |
title_fullStr | The Coxiella burnetii Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation |
title_full_unstemmed | The Coxiella burnetii Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation |
title_short | The Coxiella burnetii Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation |
title_sort | coxiella burnetii dot/icm system creates a comfortable home through lysosomal renovation |
topic | Commentary |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3195215/ https://www.ncbi.nlm.nih.gov/pubmed/22010216 http://dx.doi.org/10.1128/mBio.00226-11 |
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