Male infertility-linked point mutation disrupts the Ca(2+) oscillation-inducing and PIP(2) hydrolysis activity of sperm PLCζ

A male infertility-linked human PLCζ (phospholipase Cζ) mutation introduced into mouse PLCζ completely abolishes both in vitro PIP(2) (phosphatidylinositol 4,5-bisphosphate) hydrolysis activity and the ability to trigger in vivo Ca(2+) oscillations in mouse eggs. Wild-type PLCζ initiated a normal pa...

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Autores principales: Nomikos, Michail, Elgmati, Khalil, Theodoridou, Maria, Calver, Brian L., Cumbes, Bevan, Nounesis, Georg, Swann, Karl, Lai, F. Anthony
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2011
Materias:
Accelerated Publication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3195387/
https://www.ncbi.nlm.nih.gov/pubmed/21204786
http://dx.doi.org/10.1042/BJ20101772
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author Nomikos, Michail
Elgmati, Khalil
Theodoridou, Maria
Calver, Brian L.
Cumbes, Bevan
Nounesis, Georg
Swann, Karl
Lai, F. Anthony
author_facet Nomikos, Michail
Elgmati, Khalil
Theodoridou, Maria
Calver, Brian L.
Cumbes, Bevan
Nounesis, Georg
Swann, Karl
Lai, F. Anthony
author_sort Nomikos, Michail
collection PubMed
description A male infertility-linked human PLCζ (phospholipase Cζ) mutation introduced into mouse PLCζ completely abolishes both in vitro PIP(2) (phosphatidylinositol 4,5-bisphosphate) hydrolysis activity and the ability to trigger in vivo Ca(2+) oscillations in mouse eggs. Wild-type PLCζ initiated a normal pattern of Ca(2+) oscillations in eggs in the presence of 10-fold higher mutant PLCζ, suggesting that infertility is not mediated by a dominant-negative mechanism.
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spelling pubmed-31953872011-11-01 Male infertility-linked point mutation disrupts the Ca(2+) oscillation-inducing and PIP(2) hydrolysis activity of sperm PLCζ Nomikos, Michail Elgmati, Khalil Theodoridou, Maria Calver, Brian L. Cumbes, Bevan Nounesis, Georg Swann, Karl Lai, F. Anthony Biochem J Accelerated Publication A male infertility-linked human PLCζ (phospholipase Cζ) mutation introduced into mouse PLCζ completely abolishes both in vitro PIP(2) (phosphatidylinositol 4,5-bisphosphate) hydrolysis activity and the ability to trigger in vivo Ca(2+) oscillations in mouse eggs. Wild-type PLCζ initiated a normal pattern of Ca(2+) oscillations in eggs in the presence of 10-fold higher mutant PLCζ, suggesting that infertility is not mediated by a dominant-negative mechanism. Portland Press Ltd. 2011-02-11 2011-03-01 /pmc/articles/PMC3195387/ /pubmed/21204786 http://dx.doi.org/10.1042/BJ20101772 Text en © 2011 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by-nc/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Accelerated Publication
Nomikos, Michail
Elgmati, Khalil
Theodoridou, Maria
Calver, Brian L.
Cumbes, Bevan
Nounesis, Georg
Swann, Karl
Lai, F. Anthony
Male infertility-linked point mutation disrupts the Ca(2+) oscillation-inducing and PIP(2) hydrolysis activity of sperm PLCζ
title Male infertility-linked point mutation disrupts the Ca(2+) oscillation-inducing and PIP(2) hydrolysis activity of sperm PLCζ
title_full Male infertility-linked point mutation disrupts the Ca(2+) oscillation-inducing and PIP(2) hydrolysis activity of sperm PLCζ
title_fullStr Male infertility-linked point mutation disrupts the Ca(2+) oscillation-inducing and PIP(2) hydrolysis activity of sperm PLCζ
title_full_unstemmed Male infertility-linked point mutation disrupts the Ca(2+) oscillation-inducing and PIP(2) hydrolysis activity of sperm PLCζ
title_short Male infertility-linked point mutation disrupts the Ca(2+) oscillation-inducing and PIP(2) hydrolysis activity of sperm PLCζ
title_sort male infertility-linked point mutation disrupts the ca(2+) oscillation-inducing and pip(2) hydrolysis activity of sperm plcζ
topic Accelerated Publication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3195387/
https://www.ncbi.nlm.nih.gov/pubmed/21204786
http://dx.doi.org/10.1042/BJ20101772
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