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Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR

Introduction. Malaria is currently one of the most prevalent parasite-transmitted diseases caused by parasites of the genus Plasmodium. Misidentification of human malaria parasites especially P. knowlesi based on microscopic examination is very common. The objectives of this paper were to accurately...

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Autores principales: Joveen-Neoh, Wan Fen, Chong, Ka Lung, Wong, Clemente Michael Vui Ling, Lau, Tiek Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3195446/
https://www.ncbi.nlm.nih.gov/pubmed/22013506
http://dx.doi.org/10.1155/2011/104284
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author Joveen-Neoh, Wan Fen
Chong, Ka Lung
Wong, Clemente Michael Vui Ling
Lau, Tiek Ying
author_facet Joveen-Neoh, Wan Fen
Chong, Ka Lung
Wong, Clemente Michael Vui Ling
Lau, Tiek Ying
author_sort Joveen-Neoh, Wan Fen
collection PubMed
description Introduction. Malaria is currently one of the most prevalent parasite-transmitted diseases caused by parasites of the genus Plasmodium. Misidentification of human malaria parasites especially P. knowlesi based on microscopic examination is very common. The objectives of this paper were to accurately identify the incidence of human malaria parasites in the interior division of Sabah, Malaysian Borneo, based on small subunit ribosomal RNA (ssrRNA) and to determine the misidentification rate in human malaria parasites. Methods. Nested PCR was used to detect the presence of human malaria parasites. A total of 243 blood spot samples from patients who had requested for blood film for malaria parasite (BFMP) analyses were used in this study. Results. Nested PCR findings showed that there was no P. malariae infection while the highest prevalent malaria parasite was P. knowlesi, followed by P. vivax, P. falciparum, and mixed infection. Only 69.5% of the 243 samples giving consistent nested PCR and microscopic results. Conclusion. The preliminary findings from molecular detection of malaria showed that P. knowlesi was the most prevalent Plasmodium species in the interior division of Sabah. The findings from this paper may provide a clearer picture on the actual transmission of different Plasmodium species in this region.
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spelling pubmed-31954462011-10-19 Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR Joveen-Neoh, Wan Fen Chong, Ka Lung Wong, Clemente Michael Vui Ling Lau, Tiek Ying J Parasitol Res Research Article Introduction. Malaria is currently one of the most prevalent parasite-transmitted diseases caused by parasites of the genus Plasmodium. Misidentification of human malaria parasites especially P. knowlesi based on microscopic examination is very common. The objectives of this paper were to accurately identify the incidence of human malaria parasites in the interior division of Sabah, Malaysian Borneo, based on small subunit ribosomal RNA (ssrRNA) and to determine the misidentification rate in human malaria parasites. Methods. Nested PCR was used to detect the presence of human malaria parasites. A total of 243 blood spot samples from patients who had requested for blood film for malaria parasite (BFMP) analyses were used in this study. Results. Nested PCR findings showed that there was no P. malariae infection while the highest prevalent malaria parasite was P. knowlesi, followed by P. vivax, P. falciparum, and mixed infection. Only 69.5% of the 243 samples giving consistent nested PCR and microscopic results. Conclusion. The preliminary findings from molecular detection of malaria showed that P. knowlesi was the most prevalent Plasmodium species in the interior division of Sabah. The findings from this paper may provide a clearer picture on the actual transmission of different Plasmodium species in this region. Hindawi Publishing Corporation 2011 2011-10-13 /pmc/articles/PMC3195446/ /pubmed/22013506 http://dx.doi.org/10.1155/2011/104284 Text en Copyright © 2011 Wan Fen Joveen-Neoh et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Joveen-Neoh, Wan Fen
Chong, Ka Lung
Wong, Clemente Michael Vui Ling
Lau, Tiek Ying
Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR
title Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR
title_full Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR
title_fullStr Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR
title_full_unstemmed Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR
title_short Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR
title_sort incidence of malaria in the interior division of sabah, malaysian borneo, based on nested pcr
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3195446/
https://www.ncbi.nlm.nih.gov/pubmed/22013506
http://dx.doi.org/10.1155/2011/104284
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