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Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection
Baculovirus infection of Spodoptera frugiperda cells is a system of choice to produce a range of recombinant proteins, vaccines and, potentially, gene therapy vectors. While baculovirus genomes are well characterized, the genome of S. frugiperda is not sequenced and the virus-host molecular interpla...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3196586/ https://www.ncbi.nlm.nih.gov/pubmed/22039490 http://dx.doi.org/10.1371/journal.pone.0026444 |
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author | Carinhas, Nuno Robitaille, Aaron Mark Moes, Suzette Carrondo, Manuel José Teixeira Jenoe, Paul Oliveira, Rui Alves, Paula Marques |
author_facet | Carinhas, Nuno Robitaille, Aaron Mark Moes, Suzette Carrondo, Manuel José Teixeira Jenoe, Paul Oliveira, Rui Alves, Paula Marques |
author_sort | Carinhas, Nuno |
collection | PubMed |
description | Baculovirus infection of Spodoptera frugiperda cells is a system of choice to produce a range of recombinant proteins, vaccines and, potentially, gene therapy vectors. While baculovirus genomes are well characterized, the genome of S. frugiperda is not sequenced and the virus-host molecular interplay is sparsely known. Herein, we describe the application of stable isotope labeling by amino acids in cell culture (SILAC) to obtain the first comparative proteome quantitation of S. frugiperda cells during growth and early baculovirus infection. The proteome coverage was maximized by compiling a search database with protein annotations from insect species. Of interest were differentially proteins related to energy metabolism, endoplasmic reticulum and oxidative stress, yet not investigated in the scope of baculovirus infection. Further, the reduced expression of key viral-encoded proteins early in the infection cycle is suggested to be related with decreased viral replication at high cell density culture. These findings have implications for virological research and improvement of baculovirus-based bioprocesses. |
format | Online Article Text |
id | pubmed-3196586 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31965862011-10-28 Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection Carinhas, Nuno Robitaille, Aaron Mark Moes, Suzette Carrondo, Manuel José Teixeira Jenoe, Paul Oliveira, Rui Alves, Paula Marques PLoS One Research Article Baculovirus infection of Spodoptera frugiperda cells is a system of choice to produce a range of recombinant proteins, vaccines and, potentially, gene therapy vectors. While baculovirus genomes are well characterized, the genome of S. frugiperda is not sequenced and the virus-host molecular interplay is sparsely known. Herein, we describe the application of stable isotope labeling by amino acids in cell culture (SILAC) to obtain the first comparative proteome quantitation of S. frugiperda cells during growth and early baculovirus infection. The proteome coverage was maximized by compiling a search database with protein annotations from insect species. Of interest were differentially proteins related to energy metabolism, endoplasmic reticulum and oxidative stress, yet not investigated in the scope of baculovirus infection. Further, the reduced expression of key viral-encoded proteins early in the infection cycle is suggested to be related with decreased viral replication at high cell density culture. These findings have implications for virological research and improvement of baculovirus-based bioprocesses. Public Library of Science 2011-10-18 /pmc/articles/PMC3196586/ /pubmed/22039490 http://dx.doi.org/10.1371/journal.pone.0026444 Text en Carinhas et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Carinhas, Nuno Robitaille, Aaron Mark Moes, Suzette Carrondo, Manuel José Teixeira Jenoe, Paul Oliveira, Rui Alves, Paula Marques Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection |
title | Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection |
title_full | Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection |
title_fullStr | Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection |
title_full_unstemmed | Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection |
title_short | Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection |
title_sort | quantitative proteomics of spodoptera frugiperda cells during growth and baculovirus infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3196586/ https://www.ncbi.nlm.nih.gov/pubmed/22039490 http://dx.doi.org/10.1371/journal.pone.0026444 |
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