Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems

Repair of acute injury to the cell membrane is an elemental process of normal cellular physiology, and defective membrane repair has been linked to many degenerative human diseases. The recent discovery of MG53 as a key component of the membrane resealing machinery allows for a better molecular unde...

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Autores principales: Weisleder, Noah, Lin, Peihui, Zhao, Xiaoli, Orange, Matthew, Zhu, Hua, Ma, Jianjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2011
Materias:
Cellular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3197044/
https://www.ncbi.nlm.nih.gov/pubmed/21750489
http://dx.doi.org/10.3791/2717
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author Weisleder, Noah
Lin, Peihui
Zhao, Xiaoli
Orange, Matthew
Zhu, Hua
Ma, Jianjie
author_facet Weisleder, Noah
Lin, Peihui
Zhao, Xiaoli
Orange, Matthew
Zhu, Hua
Ma, Jianjie
author_sort Weisleder, Noah
collection PubMed
description Repair of acute injury to the cell membrane is an elemental process of normal cellular physiology, and defective membrane repair has been linked to many degenerative human diseases. The recent discovery of MG53 as a key component of the membrane resealing machinery allows for a better molecular understanding of the basic biology of tissue repair, as well as for potential translational applications in regenerative medicine. Here we detail the experimental protocols for exploring the in vivo function of MG53 in repair of muscle injury using treadmill exercise protocols on mouse models, for testing the ex vivo membrane repair capacity by measuring dye entry into isolated muscle fibers, and for monitoring the dynamic process of MG53-mediated vesicle trafficking and cell membrane repair in cultured cells using live cell confocal microscopy.
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spelling pubmed-31970442011-11-01 Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems Weisleder, Noah Lin, Peihui Zhao, Xiaoli Orange, Matthew Zhu, Hua Ma, Jianjie J Vis Exp Cellular Biology Repair of acute injury to the cell membrane is an elemental process of normal cellular physiology, and defective membrane repair has been linked to many degenerative human diseases. The recent discovery of MG53 as a key component of the membrane resealing machinery allows for a better molecular understanding of the basic biology of tissue repair, as well as for potential translational applications in regenerative medicine. Here we detail the experimental protocols for exploring the in vivo function of MG53 in repair of muscle injury using treadmill exercise protocols on mouse models, for testing the ex vivo membrane repair capacity by measuring dye entry into isolated muscle fibers, and for monitoring the dynamic process of MG53-mediated vesicle trafficking and cell membrane repair in cultured cells using live cell confocal microscopy. MyJove Corporation 2011-06-30 /pmc/articles/PMC3197044/ /pubmed/21750489 http://dx.doi.org/10.3791/2717 Text en Copyright © 2011, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Cellular Biology
Weisleder, Noah
Lin, Peihui
Zhao, Xiaoli
Orange, Matthew
Zhu, Hua
Ma, Jianjie
Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems
title Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems
title_full Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems
title_fullStr Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems
title_full_unstemmed Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems
title_short Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems
title_sort visualization of mg53-mediated cell membrane repair using in vivo and in vitro systems
topic Cellular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3197044/
https://www.ncbi.nlm.nih.gov/pubmed/21750489
http://dx.doi.org/10.3791/2717
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