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Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes

Voltage-sensitive dye imaging of neurons is a key methodology for the understanding of how neuronal networks are organised and how the simultaneous activity of participating neurons leads to the emergence of the integral functionality of the network. Here we present the methodology of application of...

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Detalles Bibliográficos
Autores principales: Stein, Wolfgang, Städele, Carola, Andras, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3197313/
https://www.ncbi.nlm.nih.gov/pubmed/21490564
http://dx.doi.org/10.3791/2567
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author Stein, Wolfgang
Städele, Carola
Andras, Peter
author_facet Stein, Wolfgang
Städele, Carola
Andras, Peter
author_sort Stein, Wolfgang
collection PubMed
description Voltage-sensitive dye imaging of neurons is a key methodology for the understanding of how neuronal networks are organised and how the simultaneous activity of participating neurons leads to the emergence of the integral functionality of the network. Here we present the methodology of application of this technique to identified pattern generating neurons in the crab stomatogastric ganglion. We demonstrate the loading of these neurons with the fluorescent voltage-sensitive dye Di-8-ANEPPQ and we show how to image the activity of dye loaded neurons using the MiCAM02 high speed and high resolution CCD camera imaging system. We demonstrate the analysis of the recorded imaging data using the BVAna imaging software associated with the MiCAM02 imaging system. The simultaneous voltage-sensitive dye imaging of the detailed activity of multiple neurons in the crab stomatogastric ganglion applied together with traditional electrophysiology techniques (intracellular and extracellular recordings) opens radically new opportunities for the understanding of how central pattern generator neural networks work.
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spelling pubmed-31973132011-10-24 Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes Stein, Wolfgang Städele, Carola Andras, Peter J Vis Exp Neuroscience Voltage-sensitive dye imaging of neurons is a key methodology for the understanding of how neuronal networks are organised and how the simultaneous activity of participating neurons leads to the emergence of the integral functionality of the network. Here we present the methodology of application of this technique to identified pattern generating neurons in the crab stomatogastric ganglion. We demonstrate the loading of these neurons with the fluorescent voltage-sensitive dye Di-8-ANEPPQ and we show how to image the activity of dye loaded neurons using the MiCAM02 high speed and high resolution CCD camera imaging system. We demonstrate the analysis of the recorded imaging data using the BVAna imaging software associated with the MiCAM02 imaging system. The simultaneous voltage-sensitive dye imaging of the detailed activity of multiple neurons in the crab stomatogastric ganglion applied together with traditional electrophysiology techniques (intracellular and extracellular recordings) opens radically new opportunities for the understanding of how central pattern generator neural networks work. MyJove Corporation 2011-03-23 /pmc/articles/PMC3197313/ /pubmed/21490564 http://dx.doi.org/10.3791/2567 Text en Copyright © 2011, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Neuroscience
Stein, Wolfgang
Städele, Carola
Andras, Peter
Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes
title Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes
title_full Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes
title_fullStr Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes
title_full_unstemmed Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes
title_short Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes
title_sort optical imaging of neurons in the crab stomatogastric ganglion with voltage-sensitive dyes
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3197313/
https://www.ncbi.nlm.nih.gov/pubmed/21490564
http://dx.doi.org/10.3791/2567
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