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Characterization of MSB Synapses in Dissociated Hippocampal Culture with Simultaneous Pre- and Postsynaptic Live Microscopy

Multisynaptic boutons (MSBs) are presynaptic boutons in contact with multiple postsynaptic partners. Although MSB synapses have been studied with static imaging techniques such as electron microscopy (EM), the dynamics of individual MSB synapses have not been directly evaluated. It is known that the...

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Autores principales: Reilly, James E., Hanson, Hugo H., Fernández-Monreal, Mónica, Wearne, Susan L., Hof, Patrick R., Phillips, Greg R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3197663/
https://www.ncbi.nlm.nih.gov/pubmed/22028887
http://dx.doi.org/10.1371/journal.pone.0026478
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author Reilly, James E.
Hanson, Hugo H.
Fernández-Monreal, Mónica
Wearne, Susan L.
Hof, Patrick R.
Phillips, Greg R.
author_facet Reilly, James E.
Hanson, Hugo H.
Fernández-Monreal, Mónica
Wearne, Susan L.
Hof, Patrick R.
Phillips, Greg R.
author_sort Reilly, James E.
collection PubMed
description Multisynaptic boutons (MSBs) are presynaptic boutons in contact with multiple postsynaptic partners. Although MSB synapses have been studied with static imaging techniques such as electron microscopy (EM), the dynamics of individual MSB synapses have not been directly evaluated. It is known that the number of MSB synapses increases with synaptogenesis and plasticity but the formation, behavior, and fate of individual MSB synapses remains largely unknown. To address this, we developed a means of live imaging MSB synapses to observe them directly over time. With time lapse confocal microscopy of GFP-filled dendrites in contact with VAMP2-DsRed-labeled boutons, we recorded both MSBs and their contacting spines hourly over 15 or more hours. Our live microscopy showed that, compared to spines contacting single synaptic boutons (SSBs), MSB-contacting spines exhibit elevated dynamic behavior. These results are consistent with the idea that MSBs serve as intermediates in synaptic development and plasticity.
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spelling pubmed-31976632011-10-25 Characterization of MSB Synapses in Dissociated Hippocampal Culture with Simultaneous Pre- and Postsynaptic Live Microscopy Reilly, James E. Hanson, Hugo H. Fernández-Monreal, Mónica Wearne, Susan L. Hof, Patrick R. Phillips, Greg R. PLoS One Research Article Multisynaptic boutons (MSBs) are presynaptic boutons in contact with multiple postsynaptic partners. Although MSB synapses have been studied with static imaging techniques such as electron microscopy (EM), the dynamics of individual MSB synapses have not been directly evaluated. It is known that the number of MSB synapses increases with synaptogenesis and plasticity but the formation, behavior, and fate of individual MSB synapses remains largely unknown. To address this, we developed a means of live imaging MSB synapses to observe them directly over time. With time lapse confocal microscopy of GFP-filled dendrites in contact with VAMP2-DsRed-labeled boutons, we recorded both MSBs and their contacting spines hourly over 15 or more hours. Our live microscopy showed that, compared to spines contacting single synaptic boutons (SSBs), MSB-contacting spines exhibit elevated dynamic behavior. These results are consistent with the idea that MSBs serve as intermediates in synaptic development and plasticity. Public Library of Science 2011-10-20 /pmc/articles/PMC3197663/ /pubmed/22028887 http://dx.doi.org/10.1371/journal.pone.0026478 Text en Reilly et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Reilly, James E.
Hanson, Hugo H.
Fernández-Monreal, Mónica
Wearne, Susan L.
Hof, Patrick R.
Phillips, Greg R.
Characterization of MSB Synapses in Dissociated Hippocampal Culture with Simultaneous Pre- and Postsynaptic Live Microscopy
title Characterization of MSB Synapses in Dissociated Hippocampal Culture with Simultaneous Pre- and Postsynaptic Live Microscopy
title_full Characterization of MSB Synapses in Dissociated Hippocampal Culture with Simultaneous Pre- and Postsynaptic Live Microscopy
title_fullStr Characterization of MSB Synapses in Dissociated Hippocampal Culture with Simultaneous Pre- and Postsynaptic Live Microscopy
title_full_unstemmed Characterization of MSB Synapses in Dissociated Hippocampal Culture with Simultaneous Pre- and Postsynaptic Live Microscopy
title_short Characterization of MSB Synapses in Dissociated Hippocampal Culture with Simultaneous Pre- and Postsynaptic Live Microscopy
title_sort characterization of msb synapses in dissociated hippocampal culture with simultaneous pre- and postsynaptic live microscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3197663/
https://www.ncbi.nlm.nih.gov/pubmed/22028887
http://dx.doi.org/10.1371/journal.pone.0026478
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