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Determinants of Functional Coupling between Astrocytes and Respiratory Neurons in the Pre-Bötzinger Complex
Respiratory neuronal network activity is thought to require efficient functioning of astrocytes. Here, we analyzed neuron-astrocyte communication in the pre-Bötzinger Complex (preBötC) of rhythmic slice preparations from neonatal mice. In astrocytes that exhibited rhythmic potassium fluxes and gluta...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198395/ https://www.ncbi.nlm.nih.gov/pubmed/22039458 http://dx.doi.org/10.1371/journal.pone.0026309 |
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author | Schnell, Christian Fresemann, Jens Hülsmann, Swen |
author_facet | Schnell, Christian Fresemann, Jens Hülsmann, Swen |
author_sort | Schnell, Christian |
collection | PubMed |
description | Respiratory neuronal network activity is thought to require efficient functioning of astrocytes. Here, we analyzed neuron-astrocyte communication in the pre-Bötzinger Complex (preBötC) of rhythmic slice preparations from neonatal mice. In astrocytes that exhibited rhythmic potassium fluxes and glutamate transporter currents, we did not find a translation of respiratory neuronal activity into phase-locked astroglial calcium signals. In up to 20% of astrocytes, 2-photon calcium imaging revealed spontaneous calcium fluctuations, although with no correlation to neuronal activity. Calcium signals could be elicited in preBötC astrocytes by metabotropic glutamate receptor activation or after inhibition of glial glutamate uptake. In the latter case, astrocyte calcium elevation preceded a surge of respiratory neuron discharge activity followed by network failure. We conclude that astrocytes do not exhibit respiratory-rhythmic calcium fluctuations when they are able to prevent synaptic glutamate accumulation. Calcium signaling is, however, observed when glutamate transport processes in astrocytes are suppressed or neuronal discharge activity is excessive. |
format | Online Article Text |
id | pubmed-3198395 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31983952011-10-28 Determinants of Functional Coupling between Astrocytes and Respiratory Neurons in the Pre-Bötzinger Complex Schnell, Christian Fresemann, Jens Hülsmann, Swen PLoS One Research Article Respiratory neuronal network activity is thought to require efficient functioning of astrocytes. Here, we analyzed neuron-astrocyte communication in the pre-Bötzinger Complex (preBötC) of rhythmic slice preparations from neonatal mice. In astrocytes that exhibited rhythmic potassium fluxes and glutamate transporter currents, we did not find a translation of respiratory neuronal activity into phase-locked astroglial calcium signals. In up to 20% of astrocytes, 2-photon calcium imaging revealed spontaneous calcium fluctuations, although with no correlation to neuronal activity. Calcium signals could be elicited in preBötC astrocytes by metabotropic glutamate receptor activation or after inhibition of glial glutamate uptake. In the latter case, astrocyte calcium elevation preceded a surge of respiratory neuron discharge activity followed by network failure. We conclude that astrocytes do not exhibit respiratory-rhythmic calcium fluctuations when they are able to prevent synaptic glutamate accumulation. Calcium signaling is, however, observed when glutamate transport processes in astrocytes are suppressed or neuronal discharge activity is excessive. Public Library of Science 2011-10-19 /pmc/articles/PMC3198395/ /pubmed/22039458 http://dx.doi.org/10.1371/journal.pone.0026309 Text en Schnell et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Schnell, Christian Fresemann, Jens Hülsmann, Swen Determinants of Functional Coupling between Astrocytes and Respiratory Neurons in the Pre-Bötzinger Complex |
title | Determinants of Functional Coupling between Astrocytes and Respiratory Neurons in the Pre-Bötzinger Complex |
title_full | Determinants of Functional Coupling between Astrocytes and Respiratory Neurons in the Pre-Bötzinger Complex |
title_fullStr | Determinants of Functional Coupling between Astrocytes and Respiratory Neurons in the Pre-Bötzinger Complex |
title_full_unstemmed | Determinants of Functional Coupling between Astrocytes and Respiratory Neurons in the Pre-Bötzinger Complex |
title_short | Determinants of Functional Coupling between Astrocytes and Respiratory Neurons in the Pre-Bötzinger Complex |
title_sort | determinants of functional coupling between astrocytes and respiratory neurons in the pre-bötzinger complex |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198395/ https://www.ncbi.nlm.nih.gov/pubmed/22039458 http://dx.doi.org/10.1371/journal.pone.0026309 |
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