Molecular-Genetic Mapping of Zebrafish Mutants with Variable Phenotypic Penetrance

Forward genetic screens in vertebrates are powerful tools to generate models relevant to human diseases, including neuropsychiatric disorders. Variability in phenotypic penetrance and expressivity is common in these disorders and behavioral mutant models, making their molecular-genetic mapping a for...

Descripción completa

Detalles Bibliográficos
Autores principales: Jain, Roshan A., Wolman, Marc A., Schmidt, Lauren A., Burgess, Harold A., Granato, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198425/
https://www.ncbi.nlm.nih.gov/pubmed/22039502
http://dx.doi.org/10.1371/journal.pone.0026510
_version_ 1782214421943156736
author Jain, Roshan A.
Wolman, Marc A.
Schmidt, Lauren A.
Burgess, Harold A.
Granato, Michael
author_facet Jain, Roshan A.
Wolman, Marc A.
Schmidt, Lauren A.
Burgess, Harold A.
Granato, Michael
author_sort Jain, Roshan A.
collection PubMed
description Forward genetic screens in vertebrates are powerful tools to generate models relevant to human diseases, including neuropsychiatric disorders. Variability in phenotypic penetrance and expressivity is common in these disorders and behavioral mutant models, making their molecular-genetic mapping a formidable task. Using a ‘phenotyping by segregation’ strategy, we molecularly map the hypersensitive zebrafish houdini mutant despite its variable phenotypic penetrance, providing a generally applicable strategy to map zebrafish mutants with subtle phenotypes.
format Online
Article
Text
id pubmed-3198425
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-31984252011-10-28 Molecular-Genetic Mapping of Zebrafish Mutants with Variable Phenotypic Penetrance Jain, Roshan A. Wolman, Marc A. Schmidt, Lauren A. Burgess, Harold A. Granato, Michael PLoS One Research Article Forward genetic screens in vertebrates are powerful tools to generate models relevant to human diseases, including neuropsychiatric disorders. Variability in phenotypic penetrance and expressivity is common in these disorders and behavioral mutant models, making their molecular-genetic mapping a formidable task. Using a ‘phenotyping by segregation’ strategy, we molecularly map the hypersensitive zebrafish houdini mutant despite its variable phenotypic penetrance, providing a generally applicable strategy to map zebrafish mutants with subtle phenotypes. Public Library of Science 2011-10-19 /pmc/articles/PMC3198425/ /pubmed/22039502 http://dx.doi.org/10.1371/journal.pone.0026510 Text en Jain et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jain, Roshan A.
Wolman, Marc A.
Schmidt, Lauren A.
Burgess, Harold A.
Granato, Michael
Molecular-Genetic Mapping of Zebrafish Mutants with Variable Phenotypic Penetrance
title Molecular-Genetic Mapping of Zebrafish Mutants with Variable Phenotypic Penetrance
title_full Molecular-Genetic Mapping of Zebrafish Mutants with Variable Phenotypic Penetrance
title_fullStr Molecular-Genetic Mapping of Zebrafish Mutants with Variable Phenotypic Penetrance
title_full_unstemmed Molecular-Genetic Mapping of Zebrafish Mutants with Variable Phenotypic Penetrance
title_short Molecular-Genetic Mapping of Zebrafish Mutants with Variable Phenotypic Penetrance
title_sort molecular-genetic mapping of zebrafish mutants with variable phenotypic penetrance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198425/
https://www.ncbi.nlm.nih.gov/pubmed/22039502
http://dx.doi.org/10.1371/journal.pone.0026510
work_keys_str_mv AT jainroshana moleculargeneticmappingofzebrafishmutantswithvariablephenotypicpenetrance
AT wolmanmarca moleculargeneticmappingofzebrafishmutantswithvariablephenotypicpenetrance
AT schmidtlaurena moleculargeneticmappingofzebrafishmutantswithvariablephenotypicpenetrance
AT burgessharolda moleculargeneticmappingofzebrafishmutantswithvariablephenotypicpenetrance
AT granatomichael moleculargeneticmappingofzebrafishmutantswithvariablephenotypicpenetrance

Ejemplares similares