High-Dose siRNAs Upregulate Mouse Eri-1 at both Transcription and Posttranscription Levels

The eri-1 gene encodes a 3′ exonuclease that can negatively regulate RNA interference via siRNase activity. High-dose siRNAs (hd-siRNAs) can enhance Eri-1 expression, which in return degrade siRNAs and greatly reduces RNAi efficiency. Here we report that hd-siRNAs induce mouse Eri-1 (meri-1) express...

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Detalles Bibliográficos
Autores principales: Bian, Yingnan, Zhou, Wei, Zhao, Yingchun, Li, Xiaoping, Geng, Wei, Hao, Ruixin, Yang, Qing, Huang, Weida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198429/
https://www.ncbi.nlm.nih.gov/pubmed/22039495
http://dx.doi.org/10.1371/journal.pone.0026466
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author Bian, Yingnan
Zhou, Wei
Zhao, Yingchun
Li, Xiaoping
Geng, Wei
Hao, Ruixin
Yang, Qing
Huang, Weida
author_facet Bian, Yingnan
Zhou, Wei
Zhao, Yingchun
Li, Xiaoping
Geng, Wei
Hao, Ruixin
Yang, Qing
Huang, Weida
author_sort Bian, Yingnan
collection PubMed
description The eri-1 gene encodes a 3′ exonuclease that can negatively regulate RNA interference via siRNase activity. High-dose siRNAs (hd-siRNAs) can enhance Eri-1 expression, which in return degrade siRNAs and greatly reduces RNAi efficiency. Here we report that hd-siRNAs induce mouse Eri-1 (meri-1) expression through the recruitment of Sp1, Ets-1, and STAT3 to the meri-1 promoter and the formation of an Sp1-Ets-1-STAT3 complex. In addition, hd-siRNAs also abolish the 3′ untranslated region (UTR) mediated posttranscriptional repression of meri-1. Our findings demonstrate the molecular mechanism underlying the upregulation of meri-1 by hd-siRNA.
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spelling pubmed-31984292011-10-28 High-Dose siRNAs Upregulate Mouse Eri-1 at both Transcription and Posttranscription Levels Bian, Yingnan Zhou, Wei Zhao, Yingchun Li, Xiaoping Geng, Wei Hao, Ruixin Yang, Qing Huang, Weida PLoS One Research Article The eri-1 gene encodes a 3′ exonuclease that can negatively regulate RNA interference via siRNase activity. High-dose siRNAs (hd-siRNAs) can enhance Eri-1 expression, which in return degrade siRNAs and greatly reduces RNAi efficiency. Here we report that hd-siRNAs induce mouse Eri-1 (meri-1) expression through the recruitment of Sp1, Ets-1, and STAT3 to the meri-1 promoter and the formation of an Sp1-Ets-1-STAT3 complex. In addition, hd-siRNAs also abolish the 3′ untranslated region (UTR) mediated posttranscriptional repression of meri-1. Our findings demonstrate the molecular mechanism underlying the upregulation of meri-1 by hd-siRNA. Public Library of Science 2011-10-19 /pmc/articles/PMC3198429/ /pubmed/22039495 http://dx.doi.org/10.1371/journal.pone.0026466 Text en Bian et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bian, Yingnan
Zhou, Wei
Zhao, Yingchun
Li, Xiaoping
Geng, Wei
Hao, Ruixin
Yang, Qing
Huang, Weida
High-Dose siRNAs Upregulate Mouse Eri-1 at both Transcription and Posttranscription Levels
title High-Dose siRNAs Upregulate Mouse Eri-1 at both Transcription and Posttranscription Levels
title_full High-Dose siRNAs Upregulate Mouse Eri-1 at both Transcription and Posttranscription Levels
title_fullStr High-Dose siRNAs Upregulate Mouse Eri-1 at both Transcription and Posttranscription Levels
title_full_unstemmed High-Dose siRNAs Upregulate Mouse Eri-1 at both Transcription and Posttranscription Levels
title_short High-Dose siRNAs Upregulate Mouse Eri-1 at both Transcription and Posttranscription Levels
title_sort high-dose sirnas upregulate mouse eri-1 at both transcription and posttranscription levels
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198429/
https://www.ncbi.nlm.nih.gov/pubmed/22039495
http://dx.doi.org/10.1371/journal.pone.0026466
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