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Geographically Widespread Swordfish Barcode Stock Identification: A Case Study of Its Application

BACKGROUND: The swordfish (Xiphias gladius) is a cosmopolitan large pelagic fish inhabiting tempered and tropical waters and it is a target species for fisheries all around the world. The present study investigated the ability of COI barcoding to reliably identify swordfish and particularly specific...

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Autores principales: Pappalardo, Anna Maria, Guarino, Francesca, Reina, Simona, Messina, Angela, De Pinto, Vito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198442/
https://www.ncbi.nlm.nih.gov/pubmed/22039414
http://dx.doi.org/10.1371/journal.pone.0025516
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author Pappalardo, Anna Maria
Guarino, Francesca
Reina, Simona
Messina, Angela
De Pinto, Vito
author_facet Pappalardo, Anna Maria
Guarino, Francesca
Reina, Simona
Messina, Angela
De Pinto, Vito
author_sort Pappalardo, Anna Maria
collection PubMed
description BACKGROUND: The swordfish (Xiphias gladius) is a cosmopolitan large pelagic fish inhabiting tempered and tropical waters and it is a target species for fisheries all around the world. The present study investigated the ability of COI barcoding to reliably identify swordfish and particularly specific stocks of this commercially important species. METHODOLOGY: We applied the classical DNA barcoding technology, upon a 682 bp segment of COI, and compared swordfish sequences from different geographical sources (Atlantic, Indian Oceans and Mediterranean Sea). The sequences of the 5′ hyper-variable fragment of the control region (5′dloop), were also used to validate the efficacy of COI as a stock-specific marker. CASE REPORT: This information was successfully applied to the discrimination of unknown samples from the market, detecting in some cases mislabeled seafood products. CONCLUSIONS: The NJ distance-based phenogram (K2P model) obtained with COI sequences allowed us to correlate the swordfish haplotypes to the different geographical stocks. Similar results were obtained with 5′dloop. Our preliminary data in swordfish Xiphias gladius confirm that Cytochrome Oxidase I can be proposed as an efficient species-specific marker that has also the potential to assign geographical provenance. This information might speed the samples analysis in commercial application of barcoding.
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spelling pubmed-31984422011-10-28 Geographically Widespread Swordfish Barcode Stock Identification: A Case Study of Its Application Pappalardo, Anna Maria Guarino, Francesca Reina, Simona Messina, Angela De Pinto, Vito PLoS One Research Article BACKGROUND: The swordfish (Xiphias gladius) is a cosmopolitan large pelagic fish inhabiting tempered and tropical waters and it is a target species for fisheries all around the world. The present study investigated the ability of COI barcoding to reliably identify swordfish and particularly specific stocks of this commercially important species. METHODOLOGY: We applied the classical DNA barcoding technology, upon a 682 bp segment of COI, and compared swordfish sequences from different geographical sources (Atlantic, Indian Oceans and Mediterranean Sea). The sequences of the 5′ hyper-variable fragment of the control region (5′dloop), were also used to validate the efficacy of COI as a stock-specific marker. CASE REPORT: This information was successfully applied to the discrimination of unknown samples from the market, detecting in some cases mislabeled seafood products. CONCLUSIONS: The NJ distance-based phenogram (K2P model) obtained with COI sequences allowed us to correlate the swordfish haplotypes to the different geographical stocks. Similar results were obtained with 5′dloop. Our preliminary data in swordfish Xiphias gladius confirm that Cytochrome Oxidase I can be proposed as an efficient species-specific marker that has also the potential to assign geographical provenance. This information might speed the samples analysis in commercial application of barcoding. Public Library of Science 2011-10-19 /pmc/articles/PMC3198442/ /pubmed/22039414 http://dx.doi.org/10.1371/journal.pone.0025516 Text en Pappalardo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Pappalardo, Anna Maria
Guarino, Francesca
Reina, Simona
Messina, Angela
De Pinto, Vito
Geographically Widespread Swordfish Barcode Stock Identification: A Case Study of Its Application
title Geographically Widespread Swordfish Barcode Stock Identification: A Case Study of Its Application
title_full Geographically Widespread Swordfish Barcode Stock Identification: A Case Study of Its Application
title_fullStr Geographically Widespread Swordfish Barcode Stock Identification: A Case Study of Its Application
title_full_unstemmed Geographically Widespread Swordfish Barcode Stock Identification: A Case Study of Its Application
title_short Geographically Widespread Swordfish Barcode Stock Identification: A Case Study of Its Application
title_sort geographically widespread swordfish barcode stock identification: a case study of its application
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198442/
https://www.ncbi.nlm.nih.gov/pubmed/22039414
http://dx.doi.org/10.1371/journal.pone.0025516
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