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Automated Detection and Segmentation of Synaptic Contacts in Nearly Isotropic Serial Electron Microscopy Images

We describe a protocol for fully automated detection and segmentation of asymmetric, presumed excitatory, synapses in serial electron microscopy images of the adult mammalian cerebral cortex, taken with the focused ion beam, scanning electron microscope (FIB/SEM). The procedure is based on interacti...

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Detalles Bibliográficos
Autores principales:	Kreshuk, Anna, Straehle, Christoph N., Sommer, Christoph, Koethe, Ullrich, Cantoni, Marco, Knott, Graham, Hamprecht, Fred A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198725/ https://www.ncbi.nlm.nih.gov/pubmed/22031814 http://dx.doi.org/10.1371/journal.pone.0024899

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