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A Comparison of Parallel Pyrosequencing and Sanger Clone-Based Sequencing and Its Impact on the Characterization of the Genetic Diversity of HIV-1
BACKGROUND: Pyrosequencing technology has the potential to rapidly sequence HIV-1 viral quasispecies without requiring the traditional approach of cloning. In this study, we investigated the utility of ultra-deep pyrosequencing to characterize genetic diversity of the HIV-1 gag quasispecies and asse...
Autores principales: | Liang, Binhua, Luo, Ma, Scott-Herridge, Joel, Semeniuk, Christina, Mendoza, Mark, Capina, Rupert, Sheardown, Brent, Ji, Hezhao, Kimani, Joshua, Ball, Blake T., Van Domselaar, Gary, Graham, Morag, Tyler, Shane, Jones, Steven J. M., Plummer, Francis A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198814/ https://www.ncbi.nlm.nih.gov/pubmed/22039546 http://dx.doi.org/10.1371/journal.pone.0026745 |
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