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L1CAM protein expression is associated with poor prognosis in non-small cell lung cancer
BACKGROUND: The L1 cell adhesion molecule (L1CAM) is potentially involved in epithelial-mesenchymal transition (EMT). EMT marker expression is of prognostic significance in non-small cell lung cancer (NSCLC). The relevance of L1CAM for NSCLC is unclear. We investigated the protein expression of L1CA...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198986/ https://www.ncbi.nlm.nih.gov/pubmed/21985405 http://dx.doi.org/10.1186/1476-4598-10-127 |
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author | Tischler, Verena Pfeifer, Marco Hausladen, Silke Schirmer, Uwe Bonde, Anne-Katrine Kristiansen, Glen Sos, Martin L Weder, Walter Moch, Holger Altevogt, Peter Soltermann, Alex |
author_facet | Tischler, Verena Pfeifer, Marco Hausladen, Silke Schirmer, Uwe Bonde, Anne-Katrine Kristiansen, Glen Sos, Martin L Weder, Walter Moch, Holger Altevogt, Peter Soltermann, Alex |
author_sort | Tischler, Verena |
collection | PubMed |
description | BACKGROUND: The L1 cell adhesion molecule (L1CAM) is potentially involved in epithelial-mesenchymal transition (EMT). EMT marker expression is of prognostic significance in non-small cell lung cancer (NSCLC). The relevance of L1CAM for NSCLC is unclear. We investigated the protein expression of L1CAM in a cohort of NSCLC patients. L1CAM protein expression was correlated with clinico-pathological parameters including survival and markers of epithelial-mesenchymal transition. RESULTS: L1CAM protein expression was found in 25% of squamous cell carcinomas and 24% of adenocarcinomas and correlated with blood vessel invasion and metastasis (p < 0.05). L1CAM was an independent predictor of survival in a multivariate analysis including pT, pN, and pM category, and tumor differentiation grade. L1CAM expression positively correlated with vimentin, beta-catenin, and slug, but inversely with E-cadherin (all p-values < 0.05). E-cadherin expression was higher in the tumor center than in the tumor periphery, whereas L1CAM and vimentin were expressed at the tumor-stroma interface. In L1CAM-negative A549 cells the L1CAM expression was upregulated and matrigel invasion was increased after stimulation with TGF-beta1. In L1CAM-positive SK-LU-1 and SK-LC-LL cells matrigel invasion was decreased after L1CAM siRNA knockdown. CONCLUSIONS: A subset of NSCLCs with vessel tropism and increased metastasis aberrantly expresses L1CAM. L1CAM is a novel prognostic marker for NSCLCs that is upregulated by EMT induction and appears to be instrumental for enhanced cell invasion. |
format | Online Article Text |
id | pubmed-3198986 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31989862011-10-23 L1CAM protein expression is associated with poor prognosis in non-small cell lung cancer Tischler, Verena Pfeifer, Marco Hausladen, Silke Schirmer, Uwe Bonde, Anne-Katrine Kristiansen, Glen Sos, Martin L Weder, Walter Moch, Holger Altevogt, Peter Soltermann, Alex Mol Cancer Research BACKGROUND: The L1 cell adhesion molecule (L1CAM) is potentially involved in epithelial-mesenchymal transition (EMT). EMT marker expression is of prognostic significance in non-small cell lung cancer (NSCLC). The relevance of L1CAM for NSCLC is unclear. We investigated the protein expression of L1CAM in a cohort of NSCLC patients. L1CAM protein expression was correlated with clinico-pathological parameters including survival and markers of epithelial-mesenchymal transition. RESULTS: L1CAM protein expression was found in 25% of squamous cell carcinomas and 24% of adenocarcinomas and correlated with blood vessel invasion and metastasis (p < 0.05). L1CAM was an independent predictor of survival in a multivariate analysis including pT, pN, and pM category, and tumor differentiation grade. L1CAM expression positively correlated with vimentin, beta-catenin, and slug, but inversely with E-cadherin (all p-values < 0.05). E-cadherin expression was higher in the tumor center than in the tumor periphery, whereas L1CAM and vimentin were expressed at the tumor-stroma interface. In L1CAM-negative A549 cells the L1CAM expression was upregulated and matrigel invasion was increased after stimulation with TGF-beta1. In L1CAM-positive SK-LU-1 and SK-LC-LL cells matrigel invasion was decreased after L1CAM siRNA knockdown. CONCLUSIONS: A subset of NSCLCs with vessel tropism and increased metastasis aberrantly expresses L1CAM. L1CAM is a novel prognostic marker for NSCLCs that is upregulated by EMT induction and appears to be instrumental for enhanced cell invasion. BioMed Central 2011-10-10 /pmc/articles/PMC3198986/ /pubmed/21985405 http://dx.doi.org/10.1186/1476-4598-10-127 Text en Copyright ©2011 Tischler et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Tischler, Verena Pfeifer, Marco Hausladen, Silke Schirmer, Uwe Bonde, Anne-Katrine Kristiansen, Glen Sos, Martin L Weder, Walter Moch, Holger Altevogt, Peter Soltermann, Alex L1CAM protein expression is associated with poor prognosis in non-small cell lung cancer |
title | L1CAM protein expression is associated with poor prognosis in non-small cell lung cancer |
title_full | L1CAM protein expression is associated with poor prognosis in non-small cell lung cancer |
title_fullStr | L1CAM protein expression is associated with poor prognosis in non-small cell lung cancer |
title_full_unstemmed | L1CAM protein expression is associated with poor prognosis in non-small cell lung cancer |
title_short | L1CAM protein expression is associated with poor prognosis in non-small cell lung cancer |
title_sort | l1cam protein expression is associated with poor prognosis in non-small cell lung cancer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198986/ https://www.ncbi.nlm.nih.gov/pubmed/21985405 http://dx.doi.org/10.1186/1476-4598-10-127 |
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