The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer

We searched for novel tumor markers of pancreatic cancer by three-step serum proteome analysis. Twelve serum abundant proteins were depleted using immunoaffinity columns followed by fractionation by reverse-phase high-performance liquid chromatography. Proteins in each fraction were separated by two...

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Autores principales: Abulaizi, Mayinuer, Tomonaga, Takeshi, Satoh, Mamoru, Sogawa, Kazuyuki, Matsushita, Kazuyuki, Kodera, Yoshio, Obul, Jurat, Takano, Shigetsugu, Yoshitomi, Hideyuki, Miyazaki, Masaru, Nomura, Fumio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199071/
https://www.ncbi.nlm.nih.gov/pubmed/22091389
http://dx.doi.org/10.1155/2011/628787
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author Abulaizi, Mayinuer
Tomonaga, Takeshi
Satoh, Mamoru
Sogawa, Kazuyuki
Matsushita, Kazuyuki
Kodera, Yoshio
Obul, Jurat
Takano, Shigetsugu
Yoshitomi, Hideyuki
Miyazaki, Masaru
Nomura, Fumio
author_facet Abulaizi, Mayinuer
Tomonaga, Takeshi
Satoh, Mamoru
Sogawa, Kazuyuki
Matsushita, Kazuyuki
Kodera, Yoshio
Obul, Jurat
Takano, Shigetsugu
Yoshitomi, Hideyuki
Miyazaki, Masaru
Nomura, Fumio
author_sort Abulaizi, Mayinuer
collection PubMed
description We searched for novel tumor markers of pancreatic cancer by three-step serum proteome analysis. Twelve serum abundant proteins were depleted using immunoaffinity columns followed by fractionation by reverse-phase high-performance liquid chromatography. Proteins in each fraction were separated by two-dimensional gel electrophoresis. Then the gel was stained by Coomassie Brilliant Blue. Protein spots in which the expression levels were significantly different between cancer and normal control were identified by LC-MS/MS. One hundred and two spots were upregulated, and 84 spots were downregulated in serum samples obtained from patients with pancreatic cancers, and 58 proteins were identified by mass spectrometry. These candidate proteins were validated using western blot analysis and enzyme-linked immunosorbent assay (ELISA). As a result of these validation process, we could confirm that the serum levels of apolipoprotein A-IV, vitamin D-binding protein, plasma retinol-binding protein 4, and tetranectin were significantly decreased in patients with pancreatic cancer.
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spelling pubmed-31990712011-11-16 The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer Abulaizi, Mayinuer Tomonaga, Takeshi Satoh, Mamoru Sogawa, Kazuyuki Matsushita, Kazuyuki Kodera, Yoshio Obul, Jurat Takano, Shigetsugu Yoshitomi, Hideyuki Miyazaki, Masaru Nomura, Fumio Int J Proteomics Research Article We searched for novel tumor markers of pancreatic cancer by three-step serum proteome analysis. Twelve serum abundant proteins were depleted using immunoaffinity columns followed by fractionation by reverse-phase high-performance liquid chromatography. Proteins in each fraction were separated by two-dimensional gel electrophoresis. Then the gel was stained by Coomassie Brilliant Blue. Protein spots in which the expression levels were significantly different between cancer and normal control were identified by LC-MS/MS. One hundred and two spots were upregulated, and 84 spots were downregulated in serum samples obtained from patients with pancreatic cancers, and 58 proteins were identified by mass spectrometry. These candidate proteins were validated using western blot analysis and enzyme-linked immunosorbent assay (ELISA). As a result of these validation process, we could confirm that the serum levels of apolipoprotein A-IV, vitamin D-binding protein, plasma retinol-binding protein 4, and tetranectin were significantly decreased in patients with pancreatic cancer. Hindawi Publishing Corporation 2011 2011-10-17 /pmc/articles/PMC3199071/ /pubmed/22091389 http://dx.doi.org/10.1155/2011/628787 Text en Copyright © 2011 Mayinuer Abulaizi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Abulaizi, Mayinuer
Tomonaga, Takeshi
Satoh, Mamoru
Sogawa, Kazuyuki
Matsushita, Kazuyuki
Kodera, Yoshio
Obul, Jurat
Takano, Shigetsugu
Yoshitomi, Hideyuki
Miyazaki, Masaru
Nomura, Fumio
The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer
title The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer
title_full The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer
title_fullStr The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer
title_full_unstemmed The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer
title_short The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer
title_sort application of a three-step proteome analysis for identification of new biomarkers of pancreatic cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199071/
https://www.ncbi.nlm.nih.gov/pubmed/22091389
http://dx.doi.org/10.1155/2011/628787
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