The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer
We searched for novel tumor markers of pancreatic cancer by three-step serum proteome analysis. Twelve serum abundant proteins were depleted using immunoaffinity columns followed by fractionation by reverse-phase high-performance liquid chromatography. Proteins in each fraction were separated by two...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199071/ https://www.ncbi.nlm.nih.gov/pubmed/22091389 http://dx.doi.org/10.1155/2011/628787 |
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author | Abulaizi, Mayinuer Tomonaga, Takeshi Satoh, Mamoru Sogawa, Kazuyuki Matsushita, Kazuyuki Kodera, Yoshio Obul, Jurat Takano, Shigetsugu Yoshitomi, Hideyuki Miyazaki, Masaru Nomura, Fumio |
author_facet | Abulaizi, Mayinuer Tomonaga, Takeshi Satoh, Mamoru Sogawa, Kazuyuki Matsushita, Kazuyuki Kodera, Yoshio Obul, Jurat Takano, Shigetsugu Yoshitomi, Hideyuki Miyazaki, Masaru Nomura, Fumio |
author_sort | Abulaizi, Mayinuer |
collection | PubMed |
description | We searched for novel tumor markers of pancreatic cancer by three-step serum proteome analysis. Twelve serum abundant proteins were depleted using immunoaffinity columns followed by fractionation by reverse-phase high-performance liquid chromatography. Proteins in each fraction were separated by two-dimensional gel electrophoresis. Then the gel was stained by Coomassie Brilliant Blue. Protein spots in which the expression levels were significantly different between cancer and normal control were identified by LC-MS/MS. One hundred and two spots were upregulated, and 84 spots were downregulated in serum samples obtained from patients with pancreatic cancers, and 58 proteins were identified by mass spectrometry. These candidate proteins were validated using western blot analysis and enzyme-linked immunosorbent assay (ELISA). As a result of these validation process, we could confirm that the serum levels of apolipoprotein A-IV, vitamin D-binding protein, plasma retinol-binding protein 4, and tetranectin were significantly decreased in patients with pancreatic cancer. |
format | Online Article Text |
id | pubmed-3199071 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-31990712011-11-16 The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer Abulaizi, Mayinuer Tomonaga, Takeshi Satoh, Mamoru Sogawa, Kazuyuki Matsushita, Kazuyuki Kodera, Yoshio Obul, Jurat Takano, Shigetsugu Yoshitomi, Hideyuki Miyazaki, Masaru Nomura, Fumio Int J Proteomics Research Article We searched for novel tumor markers of pancreatic cancer by three-step serum proteome analysis. Twelve serum abundant proteins were depleted using immunoaffinity columns followed by fractionation by reverse-phase high-performance liquid chromatography. Proteins in each fraction were separated by two-dimensional gel electrophoresis. Then the gel was stained by Coomassie Brilliant Blue. Protein spots in which the expression levels were significantly different between cancer and normal control were identified by LC-MS/MS. One hundred and two spots were upregulated, and 84 spots were downregulated in serum samples obtained from patients with pancreatic cancers, and 58 proteins were identified by mass spectrometry. These candidate proteins were validated using western blot analysis and enzyme-linked immunosorbent assay (ELISA). As a result of these validation process, we could confirm that the serum levels of apolipoprotein A-IV, vitamin D-binding protein, plasma retinol-binding protein 4, and tetranectin were significantly decreased in patients with pancreatic cancer. Hindawi Publishing Corporation 2011 2011-10-17 /pmc/articles/PMC3199071/ /pubmed/22091389 http://dx.doi.org/10.1155/2011/628787 Text en Copyright © 2011 Mayinuer Abulaizi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Abulaizi, Mayinuer Tomonaga, Takeshi Satoh, Mamoru Sogawa, Kazuyuki Matsushita, Kazuyuki Kodera, Yoshio Obul, Jurat Takano, Shigetsugu Yoshitomi, Hideyuki Miyazaki, Masaru Nomura, Fumio The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer |
title | The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer |
title_full | The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer |
title_fullStr | The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer |
title_full_unstemmed | The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer |
title_short | The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer |
title_sort | application of a three-step proteome analysis for identification of new biomarkers of pancreatic cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199071/ https://www.ncbi.nlm.nih.gov/pubmed/22091389 http://dx.doi.org/10.1155/2011/628787 |
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