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Rap2A Is Upregulated in Invasive Cells Dissected from Follicular Thyroid Cancer

The development of molecular biomarkers (BMs) of follicular thyroid carcinoma is aimed at advancing diagnosis of follicular neoplasm, as histological examination of those tumors does not lend itself to definitive diagnosis of carcinoma. We assessed the relative levels of expression of 6 genes: CCND2...

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Autores principales: Prabakaran, Indira, Grau, Jillian R., Lewis, Robert, Fraker, Douglas L., Guvakova, Marina A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199199/
https://www.ncbi.nlm.nih.gov/pubmed/22046576
http://dx.doi.org/10.4061/2011/979840
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author Prabakaran, Indira
Grau, Jillian R.
Lewis, Robert
Fraker, Douglas L.
Guvakova, Marina A.
author_facet Prabakaran, Indira
Grau, Jillian R.
Lewis, Robert
Fraker, Douglas L.
Guvakova, Marina A.
author_sort Prabakaran, Indira
collection PubMed
description The development of molecular biomarkers (BMs) of follicular thyroid carcinoma is aimed at advancing diagnosis of follicular neoplasm, as histological examination of those tumors does not lend itself to definitive diagnosis of carcinoma. We assessed the relative levels of expression of 6 genes: CCND2, PCSK2, PLAB, RAP2A, TSHR, and IGF-1R in archived thyroid tissue. The quantitative real-time PCR analysis revealed a significant change in 3 genes: PSCK2 (a 22.4-fold decrease, P = 2.81E − 2), PLAB (an 8.3-fold increase, P = 9.81E − 12), and RAP2A (a 6.3-fold increase, P = 9.13E − 10) in carcinoma compared with adenoma. Expression of PCSK2 was equally low, PLAB was equally high, whereas RAP2A expression was significantly higher (25.9-fold, P = 0.039) in microdissected carcinoma cells that have invaded through the thyroid capsule and entered blood vessels than in thyroid tumor cells growing under the capsule. Thus, RAP2A appeared as a unique and worthy of further evaluation candidate BM associated with invasion of thyroid follicular cells.
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spelling pubmed-31991992011-11-01 Rap2A Is Upregulated in Invasive Cells Dissected from Follicular Thyroid Cancer Prabakaran, Indira Grau, Jillian R. Lewis, Robert Fraker, Douglas L. Guvakova, Marina A. J Thyroid Res Research Article The development of molecular biomarkers (BMs) of follicular thyroid carcinoma is aimed at advancing diagnosis of follicular neoplasm, as histological examination of those tumors does not lend itself to definitive diagnosis of carcinoma. We assessed the relative levels of expression of 6 genes: CCND2, PCSK2, PLAB, RAP2A, TSHR, and IGF-1R in archived thyroid tissue. The quantitative real-time PCR analysis revealed a significant change in 3 genes: PSCK2 (a 22.4-fold decrease, P = 2.81E − 2), PLAB (an 8.3-fold increase, P = 9.81E − 12), and RAP2A (a 6.3-fold increase, P = 9.13E − 10) in carcinoma compared with adenoma. Expression of PCSK2 was equally low, PLAB was equally high, whereas RAP2A expression was significantly higher (25.9-fold, P = 0.039) in microdissected carcinoma cells that have invaded through the thyroid capsule and entered blood vessels than in thyroid tumor cells growing under the capsule. Thus, RAP2A appeared as a unique and worthy of further evaluation candidate BM associated with invasion of thyroid follicular cells. SAGE-Hindawi Access to Research 2011 2011-10-20 /pmc/articles/PMC3199199/ /pubmed/22046576 http://dx.doi.org/10.4061/2011/979840 Text en Copyright © 2011 Indira Prabakaran et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Prabakaran, Indira
Grau, Jillian R.
Lewis, Robert
Fraker, Douglas L.
Guvakova, Marina A.
Rap2A Is Upregulated in Invasive Cells Dissected from Follicular Thyroid Cancer
title Rap2A Is Upregulated in Invasive Cells Dissected from Follicular Thyroid Cancer
title_full Rap2A Is Upregulated in Invasive Cells Dissected from Follicular Thyroid Cancer
title_fullStr Rap2A Is Upregulated in Invasive Cells Dissected from Follicular Thyroid Cancer
title_full_unstemmed Rap2A Is Upregulated in Invasive Cells Dissected from Follicular Thyroid Cancer
title_short Rap2A Is Upregulated in Invasive Cells Dissected from Follicular Thyroid Cancer
title_sort rap2a is upregulated in invasive cells dissected from follicular thyroid cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199199/
https://www.ncbi.nlm.nih.gov/pubmed/22046576
http://dx.doi.org/10.4061/2011/979840
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