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Rap2A Is Upregulated in Invasive Cells Dissected from Follicular Thyroid Cancer
The development of molecular biomarkers (BMs) of follicular thyroid carcinoma is aimed at advancing diagnosis of follicular neoplasm, as histological examination of those tumors does not lend itself to definitive diagnosis of carcinoma. We assessed the relative levels of expression of 6 genes: CCND2...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE-Hindawi Access to Research
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199199/ https://www.ncbi.nlm.nih.gov/pubmed/22046576 http://dx.doi.org/10.4061/2011/979840 |
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author | Prabakaran, Indira Grau, Jillian R. Lewis, Robert Fraker, Douglas L. Guvakova, Marina A. |
author_facet | Prabakaran, Indira Grau, Jillian R. Lewis, Robert Fraker, Douglas L. Guvakova, Marina A. |
author_sort | Prabakaran, Indira |
collection | PubMed |
description | The development of molecular biomarkers (BMs) of follicular thyroid carcinoma is aimed at advancing diagnosis of follicular neoplasm, as histological examination of those tumors does not lend itself to definitive diagnosis of carcinoma. We assessed the relative levels of expression of 6 genes: CCND2, PCSK2, PLAB, RAP2A, TSHR, and IGF-1R in archived thyroid tissue. The quantitative real-time PCR analysis revealed a significant change in 3 genes: PSCK2 (a 22.4-fold decrease, P = 2.81E − 2), PLAB (an 8.3-fold increase, P = 9.81E − 12), and RAP2A (a 6.3-fold increase, P = 9.13E − 10) in carcinoma compared with adenoma. Expression of PCSK2 was equally low, PLAB was equally high, whereas RAP2A expression was significantly higher (25.9-fold, P = 0.039) in microdissected carcinoma cells that have invaded through the thyroid capsule and entered blood vessels than in thyroid tumor cells growing under the capsule. Thus, RAP2A appeared as a unique and worthy of further evaluation candidate BM associated with invasion of thyroid follicular cells. |
format | Online Article Text |
id | pubmed-3199199 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | SAGE-Hindawi Access to Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-31991992011-11-01 Rap2A Is Upregulated in Invasive Cells Dissected from Follicular Thyroid Cancer Prabakaran, Indira Grau, Jillian R. Lewis, Robert Fraker, Douglas L. Guvakova, Marina A. J Thyroid Res Research Article The development of molecular biomarkers (BMs) of follicular thyroid carcinoma is aimed at advancing diagnosis of follicular neoplasm, as histological examination of those tumors does not lend itself to definitive diagnosis of carcinoma. We assessed the relative levels of expression of 6 genes: CCND2, PCSK2, PLAB, RAP2A, TSHR, and IGF-1R in archived thyroid tissue. The quantitative real-time PCR analysis revealed a significant change in 3 genes: PSCK2 (a 22.4-fold decrease, P = 2.81E − 2), PLAB (an 8.3-fold increase, P = 9.81E − 12), and RAP2A (a 6.3-fold increase, P = 9.13E − 10) in carcinoma compared with adenoma. Expression of PCSK2 was equally low, PLAB was equally high, whereas RAP2A expression was significantly higher (25.9-fold, P = 0.039) in microdissected carcinoma cells that have invaded through the thyroid capsule and entered blood vessels than in thyroid tumor cells growing under the capsule. Thus, RAP2A appeared as a unique and worthy of further evaluation candidate BM associated with invasion of thyroid follicular cells. SAGE-Hindawi Access to Research 2011 2011-10-20 /pmc/articles/PMC3199199/ /pubmed/22046576 http://dx.doi.org/10.4061/2011/979840 Text en Copyright © 2011 Indira Prabakaran et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Prabakaran, Indira Grau, Jillian R. Lewis, Robert Fraker, Douglas L. Guvakova, Marina A. Rap2A Is Upregulated in Invasive Cells Dissected from Follicular Thyroid Cancer |
title |
Rap2A Is Upregulated in Invasive Cells Dissected from Follicular Thyroid Cancer |
title_full |
Rap2A Is Upregulated in Invasive Cells Dissected from Follicular Thyroid Cancer |
title_fullStr |
Rap2A Is Upregulated in Invasive Cells Dissected from Follicular Thyroid Cancer |
title_full_unstemmed |
Rap2A Is Upregulated in Invasive Cells Dissected from Follicular Thyroid Cancer |
title_short |
Rap2A Is Upregulated in Invasive Cells Dissected from Follicular Thyroid Cancer |
title_sort | rap2a is upregulated in invasive cells dissected from follicular thyroid cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199199/ https://www.ncbi.nlm.nih.gov/pubmed/22046576 http://dx.doi.org/10.4061/2011/979840 |
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