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Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction

BACKGROUND: It has been well documented that pre-eclampsia and unexplained fetal growth restriction (FGR) have a common etiological background, but little is known about their linkage at the molecular level. The aim of this study was to further investigate the mechanisms underlying pre-eclampsia and...

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Autores principales: Nishizawa, Haruki, Ota, Sayuri, Suzuki, Machiko, Kato, Takema, Sekiya, Takao, Kurahashi, Hiroki, Udagawa, Yasuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199758/
https://www.ncbi.nlm.nih.gov/pubmed/21810232
http://dx.doi.org/10.1186/1477-7827-9-107
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author Nishizawa, Haruki
Ota, Sayuri
Suzuki, Machiko
Kato, Takema
Sekiya, Takao
Kurahashi, Hiroki
Udagawa, Yasuhiro
author_facet Nishizawa, Haruki
Ota, Sayuri
Suzuki, Machiko
Kato, Takema
Sekiya, Takao
Kurahashi, Hiroki
Udagawa, Yasuhiro
author_sort Nishizawa, Haruki
collection PubMed
description BACKGROUND: It has been well documented that pre-eclampsia and unexplained fetal growth restriction (FGR) have a common etiological background, but little is known about their linkage at the molecular level. The aim of this study was to further investigate the mechanisms underlying pre-eclampsia and unexplained FGR. METHODS: We analyzed differentially expressed genes in placental tissue from severe pre-eclamptic pregnancies (n = 8) and normotensive pregnancies with or (n = 8) without FGR (n = 8) using a microarray method. RESULTS: A subset of the FGR samples showed a high correlation coefficient overall in the microarray data from the pre-eclampsia samples. Many genes that are known to be up-regulated in pre-eclampsia are also up-regulated in FGR, including the anti-angiogenic factors, FLT1 and ENG, believed to be associated with the onset of maternal symptoms of pre-eclampsia. A total of 62 genes were found to be differentially expressed in both disorders. However, gene set enrichment analysis for these differentially expressed genes further revealed higher expression of TP53-downstream genes in pre-eclampsia compared with FGR. TP53-downstream apoptosis-related genes, such as BCL6 and BAX, were found to be significantly more up-regulated in pre-eclampsia than in FGR, although the caspases are expressed at equivalent levels. CONCLUSIONS: Our current data indicate a common pathophysiology for FGR and pre-eclampsia, leading to an up-regulation of placental anti-angiogenic factors. However, our findings also suggest that it may possibly be the excretion of these factors into the maternal circulation through the TP53-mediated early-stage apoptosis of trophoblasts that leads to the maternal symptoms of pre-eclampsia.
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spelling pubmed-31997582011-10-24 Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction Nishizawa, Haruki Ota, Sayuri Suzuki, Machiko Kato, Takema Sekiya, Takao Kurahashi, Hiroki Udagawa, Yasuhiro Reprod Biol Endocrinol Research BACKGROUND: It has been well documented that pre-eclampsia and unexplained fetal growth restriction (FGR) have a common etiological background, but little is known about their linkage at the molecular level. The aim of this study was to further investigate the mechanisms underlying pre-eclampsia and unexplained FGR. METHODS: We analyzed differentially expressed genes in placental tissue from severe pre-eclamptic pregnancies (n = 8) and normotensive pregnancies with or (n = 8) without FGR (n = 8) using a microarray method. RESULTS: A subset of the FGR samples showed a high correlation coefficient overall in the microarray data from the pre-eclampsia samples. Many genes that are known to be up-regulated in pre-eclampsia are also up-regulated in FGR, including the anti-angiogenic factors, FLT1 and ENG, believed to be associated with the onset of maternal symptoms of pre-eclampsia. A total of 62 genes were found to be differentially expressed in both disorders. However, gene set enrichment analysis for these differentially expressed genes further revealed higher expression of TP53-downstream genes in pre-eclampsia compared with FGR. TP53-downstream apoptosis-related genes, such as BCL6 and BAX, were found to be significantly more up-regulated in pre-eclampsia than in FGR, although the caspases are expressed at equivalent levels. CONCLUSIONS: Our current data indicate a common pathophysiology for FGR and pre-eclampsia, leading to an up-regulation of placental anti-angiogenic factors. However, our findings also suggest that it may possibly be the excretion of these factors into the maternal circulation through the TP53-mediated early-stage apoptosis of trophoblasts that leads to the maternal symptoms of pre-eclampsia. BioMed Central 2011-08-02 /pmc/articles/PMC3199758/ /pubmed/21810232 http://dx.doi.org/10.1186/1477-7827-9-107 Text en Copyright ©2011 Nishizawa et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Nishizawa, Haruki
Ota, Sayuri
Suzuki, Machiko
Kato, Takema
Sekiya, Takao
Kurahashi, Hiroki
Udagawa, Yasuhiro
Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction
title Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction
title_full Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction
title_fullStr Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction
title_full_unstemmed Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction
title_short Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction
title_sort comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199758/
https://www.ncbi.nlm.nih.gov/pubmed/21810232
http://dx.doi.org/10.1186/1477-7827-9-107
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