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Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction
BACKGROUND: It has been well documented that pre-eclampsia and unexplained fetal growth restriction (FGR) have a common etiological background, but little is known about their linkage at the molecular level. The aim of this study was to further investigate the mechanisms underlying pre-eclampsia and...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199758/ https://www.ncbi.nlm.nih.gov/pubmed/21810232 http://dx.doi.org/10.1186/1477-7827-9-107 |
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author | Nishizawa, Haruki Ota, Sayuri Suzuki, Machiko Kato, Takema Sekiya, Takao Kurahashi, Hiroki Udagawa, Yasuhiro |
author_facet | Nishizawa, Haruki Ota, Sayuri Suzuki, Machiko Kato, Takema Sekiya, Takao Kurahashi, Hiroki Udagawa, Yasuhiro |
author_sort | Nishizawa, Haruki |
collection | PubMed |
description | BACKGROUND: It has been well documented that pre-eclampsia and unexplained fetal growth restriction (FGR) have a common etiological background, but little is known about their linkage at the molecular level. The aim of this study was to further investigate the mechanisms underlying pre-eclampsia and unexplained FGR. METHODS: We analyzed differentially expressed genes in placental tissue from severe pre-eclamptic pregnancies (n = 8) and normotensive pregnancies with or (n = 8) without FGR (n = 8) using a microarray method. RESULTS: A subset of the FGR samples showed a high correlation coefficient overall in the microarray data from the pre-eclampsia samples. Many genes that are known to be up-regulated in pre-eclampsia are also up-regulated in FGR, including the anti-angiogenic factors, FLT1 and ENG, believed to be associated with the onset of maternal symptoms of pre-eclampsia. A total of 62 genes were found to be differentially expressed in both disorders. However, gene set enrichment analysis for these differentially expressed genes further revealed higher expression of TP53-downstream genes in pre-eclampsia compared with FGR. TP53-downstream apoptosis-related genes, such as BCL6 and BAX, were found to be significantly more up-regulated in pre-eclampsia than in FGR, although the caspases are expressed at equivalent levels. CONCLUSIONS: Our current data indicate a common pathophysiology for FGR and pre-eclampsia, leading to an up-regulation of placental anti-angiogenic factors. However, our findings also suggest that it may possibly be the excretion of these factors into the maternal circulation through the TP53-mediated early-stage apoptosis of trophoblasts that leads to the maternal symptoms of pre-eclampsia. |
format | Online Article Text |
id | pubmed-3199758 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31997582011-10-24 Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction Nishizawa, Haruki Ota, Sayuri Suzuki, Machiko Kato, Takema Sekiya, Takao Kurahashi, Hiroki Udagawa, Yasuhiro Reprod Biol Endocrinol Research BACKGROUND: It has been well documented that pre-eclampsia and unexplained fetal growth restriction (FGR) have a common etiological background, but little is known about their linkage at the molecular level. The aim of this study was to further investigate the mechanisms underlying pre-eclampsia and unexplained FGR. METHODS: We analyzed differentially expressed genes in placental tissue from severe pre-eclamptic pregnancies (n = 8) and normotensive pregnancies with or (n = 8) without FGR (n = 8) using a microarray method. RESULTS: A subset of the FGR samples showed a high correlation coefficient overall in the microarray data from the pre-eclampsia samples. Many genes that are known to be up-regulated in pre-eclampsia are also up-regulated in FGR, including the anti-angiogenic factors, FLT1 and ENG, believed to be associated with the onset of maternal symptoms of pre-eclampsia. A total of 62 genes were found to be differentially expressed in both disorders. However, gene set enrichment analysis for these differentially expressed genes further revealed higher expression of TP53-downstream genes in pre-eclampsia compared with FGR. TP53-downstream apoptosis-related genes, such as BCL6 and BAX, were found to be significantly more up-regulated in pre-eclampsia than in FGR, although the caspases are expressed at equivalent levels. CONCLUSIONS: Our current data indicate a common pathophysiology for FGR and pre-eclampsia, leading to an up-regulation of placental anti-angiogenic factors. However, our findings also suggest that it may possibly be the excretion of these factors into the maternal circulation through the TP53-mediated early-stage apoptosis of trophoblasts that leads to the maternal symptoms of pre-eclampsia. BioMed Central 2011-08-02 /pmc/articles/PMC3199758/ /pubmed/21810232 http://dx.doi.org/10.1186/1477-7827-9-107 Text en Copyright ©2011 Nishizawa et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Nishizawa, Haruki Ota, Sayuri Suzuki, Machiko Kato, Takema Sekiya, Takao Kurahashi, Hiroki Udagawa, Yasuhiro Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction |
title | Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction |
title_full | Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction |
title_fullStr | Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction |
title_full_unstemmed | Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction |
title_short | Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction |
title_sort | comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199758/ https://www.ncbi.nlm.nih.gov/pubmed/21810232 http://dx.doi.org/10.1186/1477-7827-9-107 |
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