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Metabolic alteration of urinary steroids in pre- and post-menopausal women, and men with papillary thyroid carcinoma

BACKGROUND: To evaluate the metabolic changes in urinary steroids in pre- and post-menopausal women and men with papillary thyroid carcinoma (PTC). METHODS: Quantitative steroid profiling combined with gas chromatography-mass spectrometry was used to measure the urinary concentrations of 84 steroids...

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Autores principales: Choi, Man Ho, Moon, Ju-Yeon, Cho, Sung-Hee, Chung, Bong Chul, Lee, Eun Jig
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199870/
https://www.ncbi.nlm.nih.gov/pubmed/21824401
http://dx.doi.org/10.1186/1471-2407-11-342
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author Choi, Man Ho
Moon, Ju-Yeon
Cho, Sung-Hee
Chung, Bong Chul
Lee, Eun Jig
author_facet Choi, Man Ho
Moon, Ju-Yeon
Cho, Sung-Hee
Chung, Bong Chul
Lee, Eun Jig
author_sort Choi, Man Ho
collection PubMed
description BACKGROUND: To evaluate the metabolic changes in urinary steroids in pre- and post-menopausal women and men with papillary thyroid carcinoma (PTC). METHODS: Quantitative steroid profiling combined with gas chromatography-mass spectrometry was used to measure the urinary concentrations of 84 steroids in both pre- (n = 21, age: 36.95 ± 7.19 yr) and post-menopausal female (n = 19, age: 52.79 ± 7.66 yr), and male (n = 16, age: 41.88 ± 8.48 yr) patients with PTC. After comparing the quantitative data of the patients with their corresponding controls (pre-menopause women: n = 24, age: 33.21 ± 10.48 yr, post-menopause women: n = 16, age: 49.67 ± 8.94 yr, male: n = 20, age: 42.75 ± 4.22 yr), the levels of steroids in the patients were normalized to the mean concentration of the controls to exclude gender and menopausal variations. RESULTS: Many urinary steroids were up-regulated in all PTC patients compared to the controls. Among them, the levels of three active androgens, androstenedione, androstenediol and 16α-hydroxy DHEA, were significantly higher in the pre-menopausal women and men with PTC. The corticoid levels were increased slightly in the PTC men, while progestins were not altered in the post-menopausal PTC women. Estrogens were up-regulated in all PTC patients but 2-hydroxyestrone and 2-hydroxy-17β-estradiol were remarkably changed in both pre-menopausal women and men with PTC. For both menopausal and gender differences, the 2-hydroxylation, 4-hydroxylation, 2-methoxylation, and 4-methoxylation of estrogens and 16α-hydroxylation of DHEA were differentiated between pre- and post-menopausal PTC women (P < 0.001). In particular, the metabolic ratio of 2-hydroxyestrone to 2-hydroxy-17β-estradiol, which could reveal the enzyme activity of 17β-hydroxysteroid dehydrogenase, showed gender differences in PTC patients (P < 1 × 10(-7)). CONCLUSIONS: These results are expected be helpful for better understanding the pathogenic differences in PTC according to gender and menopausal conditions.
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spelling pubmed-31998702011-10-25 Metabolic alteration of urinary steroids in pre- and post-menopausal women, and men with papillary thyroid carcinoma Choi, Man Ho Moon, Ju-Yeon Cho, Sung-Hee Chung, Bong Chul Lee, Eun Jig BMC Cancer Research Article BACKGROUND: To evaluate the metabolic changes in urinary steroids in pre- and post-menopausal women and men with papillary thyroid carcinoma (PTC). METHODS: Quantitative steroid profiling combined with gas chromatography-mass spectrometry was used to measure the urinary concentrations of 84 steroids in both pre- (n = 21, age: 36.95 ± 7.19 yr) and post-menopausal female (n = 19, age: 52.79 ± 7.66 yr), and male (n = 16, age: 41.88 ± 8.48 yr) patients with PTC. After comparing the quantitative data of the patients with their corresponding controls (pre-menopause women: n = 24, age: 33.21 ± 10.48 yr, post-menopause women: n = 16, age: 49.67 ± 8.94 yr, male: n = 20, age: 42.75 ± 4.22 yr), the levels of steroids in the patients were normalized to the mean concentration of the controls to exclude gender and menopausal variations. RESULTS: Many urinary steroids were up-regulated in all PTC patients compared to the controls. Among them, the levels of three active androgens, androstenedione, androstenediol and 16α-hydroxy DHEA, were significantly higher in the pre-menopausal women and men with PTC. The corticoid levels were increased slightly in the PTC men, while progestins were not altered in the post-menopausal PTC women. Estrogens were up-regulated in all PTC patients but 2-hydroxyestrone and 2-hydroxy-17β-estradiol were remarkably changed in both pre-menopausal women and men with PTC. For both menopausal and gender differences, the 2-hydroxylation, 4-hydroxylation, 2-methoxylation, and 4-methoxylation of estrogens and 16α-hydroxylation of DHEA were differentiated between pre- and post-menopausal PTC women (P < 0.001). In particular, the metabolic ratio of 2-hydroxyestrone to 2-hydroxy-17β-estradiol, which could reveal the enzyme activity of 17β-hydroxysteroid dehydrogenase, showed gender differences in PTC patients (P < 1 × 10(-7)). CONCLUSIONS: These results are expected be helpful for better understanding the pathogenic differences in PTC according to gender and menopausal conditions. BioMed Central 2011-08-08 /pmc/articles/PMC3199870/ /pubmed/21824401 http://dx.doi.org/10.1186/1471-2407-11-342 Text en Copyright ©2011 Choi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Choi, Man Ho
Moon, Ju-Yeon
Cho, Sung-Hee
Chung, Bong Chul
Lee, Eun Jig
Metabolic alteration of urinary steroids in pre- and post-menopausal women, and men with papillary thyroid carcinoma
title Metabolic alteration of urinary steroids in pre- and post-menopausal women, and men with papillary thyroid carcinoma
title_full Metabolic alteration of urinary steroids in pre- and post-menopausal women, and men with papillary thyroid carcinoma
title_fullStr Metabolic alteration of urinary steroids in pre- and post-menopausal women, and men with papillary thyroid carcinoma
title_full_unstemmed Metabolic alteration of urinary steroids in pre- and post-menopausal women, and men with papillary thyroid carcinoma
title_short Metabolic alteration of urinary steroids in pre- and post-menopausal women, and men with papillary thyroid carcinoma
title_sort metabolic alteration of urinary steroids in pre- and post-menopausal women, and men with papillary thyroid carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199870/
https://www.ncbi.nlm.nih.gov/pubmed/21824401
http://dx.doi.org/10.1186/1471-2407-11-342
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