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Using Self-Assembled Monolayers To Understand α8β1-Mediated Cell Adhesion to RGD and FEI Motifs in Nephronectin

[Image: see text] Nephronectin is an extracellular matrix protein that interacts with the α8β1 integrin receptor and plays a role in tissue and organ development, though the motifs that mediate adhesion to the receptor remain unclear. This paper describes the use of self-assembled monolayers to stud...

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Autores principales: Sánchez-Cortés, Juan, Mrksich, Milan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2011
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3200005/
https://www.ncbi.nlm.nih.gov/pubmed/21790180
http://dx.doi.org/10.1021/cb200186j
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author Sánchez-Cortés, Juan
Mrksich, Milan
author_facet Sánchez-Cortés, Juan
Mrksich, Milan
author_sort Sánchez-Cortés, Juan
collection PubMed
description [Image: see text] Nephronectin is an extracellular matrix protein that interacts with the α8β1 integrin receptor and plays a role in tissue and organ development, though the motifs that mediate adhesion to the receptor remain unclear. This paper describes the use of self-assembled monolayers to study the adhesion of α8β1-presenting cells to the RGD and DLFEIFEIER ligands in nephronectin and found that both ligands can independently mediate cell adhesion through nonoverlapping binding sites on the integrin. Peptide truncation experiments showed FEI to be the minimal binding sequence within the DLFEIFEIER sequence, and adhesion experiments with peptides that include both the RGD and FEI sequences demonstrate that the two peptides bind synergistically to the receptor. Finally, a peptide array was used to establish a strict requirement for the glutamate residue of FEI and tolerance of other aromatic and hydrophobic residues in the first and third positions, respectively. This work provides an enhanced understanding of the binding of nephronectin with α8β1 and identifies a peptide ligand that can be used for targeting the α8β1 integrin.
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spelling pubmed-32000052011-10-24 Using Self-Assembled Monolayers To Understand α8β1-Mediated Cell Adhesion to RGD and FEI Motifs in Nephronectin Sánchez-Cortés, Juan Mrksich, Milan ACS Chem Biol [Image: see text] Nephronectin is an extracellular matrix protein that interacts with the α8β1 integrin receptor and plays a role in tissue and organ development, though the motifs that mediate adhesion to the receptor remain unclear. This paper describes the use of self-assembled monolayers to study the adhesion of α8β1-presenting cells to the RGD and DLFEIFEIER ligands in nephronectin and found that both ligands can independently mediate cell adhesion through nonoverlapping binding sites on the integrin. Peptide truncation experiments showed FEI to be the minimal binding sequence within the DLFEIFEIER sequence, and adhesion experiments with peptides that include both the RGD and FEI sequences demonstrate that the two peptides bind synergistically to the receptor. Finally, a peptide array was used to establish a strict requirement for the glutamate residue of FEI and tolerance of other aromatic and hydrophobic residues in the first and third positions, respectively. This work provides an enhanced understanding of the binding of nephronectin with α8β1 and identifies a peptide ligand that can be used for targeting the α8β1 integrin. American Chemical Society 2011-07-26 2011-10-21 /pmc/articles/PMC3200005/ /pubmed/21790180 http://dx.doi.org/10.1021/cb200186j Text en Copyright © 2011 American Chemical Society http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.
spellingShingle Sánchez-Cortés, Juan
Mrksich, Milan
Using Self-Assembled Monolayers To Understand α8β1-Mediated Cell Adhesion to RGD and FEI Motifs in Nephronectin
title Using Self-Assembled Monolayers To Understand α8β1-Mediated Cell Adhesion to RGD and FEI Motifs in Nephronectin
title_full Using Self-Assembled Monolayers To Understand α8β1-Mediated Cell Adhesion to RGD and FEI Motifs in Nephronectin
title_fullStr Using Self-Assembled Monolayers To Understand α8β1-Mediated Cell Adhesion to RGD and FEI Motifs in Nephronectin
title_full_unstemmed Using Self-Assembled Monolayers To Understand α8β1-Mediated Cell Adhesion to RGD and FEI Motifs in Nephronectin
title_short Using Self-Assembled Monolayers To Understand α8β1-Mediated Cell Adhesion to RGD and FEI Motifs in Nephronectin
title_sort using self-assembled monolayers to understand α8β1-mediated cell adhesion to rgd and fei motifs in nephronectin
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3200005/
https://www.ncbi.nlm.nih.gov/pubmed/21790180
http://dx.doi.org/10.1021/cb200186j
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