Cargando…

Rheumatoid Factor and Its Interference with Cytokine Measurements: Problems and Solutions

Use of cytokines as biomarkers for disease is getting more widespread. Cytokines are conveniently determined by immunoassay, but interference from present antibodies is known to cause problems. In rheumatoid arthritis (RA), interference of rheumatoid factor (RF) may be problematic. RF covers a group...

Descripción completa

Detalles Bibliográficos
Autores principales: Bartels, Else Marie, Falbe Wätjen, Inger, Littrup Andersen, Eva, Danneskiold-Samsøe, Bente, Bliddal, Henning, Ribel-Madsen, Søren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3200114/
https://www.ncbi.nlm.nih.gov/pubmed/22046523
http://dx.doi.org/10.1155/2011/741071
_version_ 1782214651542503424
author Bartels, Else Marie
Falbe Wätjen, Inger
Littrup Andersen, Eva
Danneskiold-Samsøe, Bente
Bliddal, Henning
Ribel-Madsen, Søren
author_facet Bartels, Else Marie
Falbe Wätjen, Inger
Littrup Andersen, Eva
Danneskiold-Samsøe, Bente
Bliddal, Henning
Ribel-Madsen, Søren
author_sort Bartels, Else Marie
collection PubMed
description Use of cytokines as biomarkers for disease is getting more widespread. Cytokines are conveniently determined by immunoassay, but interference from present antibodies is known to cause problems. In rheumatoid arthritis (RA), interference of rheumatoid factor (RF) may be problematic. RF covers a group of autoantibodies from immunoglobulin subclasses and is present in 65–80% of RA patients. Partly removal of RF is possible by precipitation. This study aims at determining the effects of presence of RF in blood and synovial fluid on cytokine measurements in samples from RA patients and finding possible solutions for recognized problems. IL-1β, IL-4, IL-6, and IL-8 were determined with multiplex immunoassays (MIA) in samples from RA patients prior to and after polyethylene glycol (PEG 6000) precipitation. Presence of RF does interfere with MIA. PEG 6000 precipitation abolishes this RF interference. We recommend PEG precipitation for all immunoassay measurements of plasma samples from RA patients.
format Online
Article
Text
id pubmed-3200114
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-32001142011-11-01 Rheumatoid Factor and Its Interference with Cytokine Measurements: Problems and Solutions Bartels, Else Marie Falbe Wätjen, Inger Littrup Andersen, Eva Danneskiold-Samsøe, Bente Bliddal, Henning Ribel-Madsen, Søren Arthritis Research Article Use of cytokines as biomarkers for disease is getting more widespread. Cytokines are conveniently determined by immunoassay, but interference from present antibodies is known to cause problems. In rheumatoid arthritis (RA), interference of rheumatoid factor (RF) may be problematic. RF covers a group of autoantibodies from immunoglobulin subclasses and is present in 65–80% of RA patients. Partly removal of RF is possible by precipitation. This study aims at determining the effects of presence of RF in blood and synovial fluid on cytokine measurements in samples from RA patients and finding possible solutions for recognized problems. IL-1β, IL-4, IL-6, and IL-8 were determined with multiplex immunoassays (MIA) in samples from RA patients prior to and after polyethylene glycol (PEG 6000) precipitation. Presence of RF does interfere with MIA. PEG 6000 precipitation abolishes this RF interference. We recommend PEG precipitation for all immunoassay measurements of plasma samples from RA patients. Hindawi Publishing Corporation 2011 2011-06-22 /pmc/articles/PMC3200114/ /pubmed/22046523 http://dx.doi.org/10.1155/2011/741071 Text en Copyright © 2011 Else Marie Bartels et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bartels, Else Marie
Falbe Wätjen, Inger
Littrup Andersen, Eva
Danneskiold-Samsøe, Bente
Bliddal, Henning
Ribel-Madsen, Søren
Rheumatoid Factor and Its Interference with Cytokine Measurements: Problems and Solutions
title Rheumatoid Factor and Its Interference with Cytokine Measurements: Problems and Solutions
title_full Rheumatoid Factor and Its Interference with Cytokine Measurements: Problems and Solutions
title_fullStr Rheumatoid Factor and Its Interference with Cytokine Measurements: Problems and Solutions
title_full_unstemmed Rheumatoid Factor and Its Interference with Cytokine Measurements: Problems and Solutions
title_short Rheumatoid Factor and Its Interference with Cytokine Measurements: Problems and Solutions
title_sort rheumatoid factor and its interference with cytokine measurements: problems and solutions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3200114/
https://www.ncbi.nlm.nih.gov/pubmed/22046523
http://dx.doi.org/10.1155/2011/741071
work_keys_str_mv AT bartelselsemarie rheumatoidfactoranditsinterferencewithcytokinemeasurementsproblemsandsolutions
AT falbewatjeninger rheumatoidfactoranditsinterferencewithcytokinemeasurementsproblemsandsolutions
AT littrupanderseneva rheumatoidfactoranditsinterferencewithcytokinemeasurementsproblemsandsolutions
AT danneskioldsamsøebente rheumatoidfactoranditsinterferencewithcytokinemeasurementsproblemsandsolutions
AT bliddalhenning rheumatoidfactoranditsinterferencewithcytokinemeasurementsproblemsandsolutions
AT ribelmadsensøren rheumatoidfactoranditsinterferencewithcytokinemeasurementsproblemsandsolutions