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Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes: Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating

One possible factor determining recovery of trace amount of protein biomarker candidates during proteome analyses could be adsorption on urine tubes. This issue, however, has not been well addressed so far. Recently, a new technical device of surface coating by poly(2-methacryloyloxyethyl phosphoryl...

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Autores principales: Kiyokawa, Iwao, Sogawa, Kazuyuki, Ise, Keiko, Iida, Fumie, Satoh, Mamoru, Miura, Toshihide, Kojima, Ryo, Katayama, Katsuhiro, Nomura, Fumio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3200127/
https://www.ncbi.nlm.nih.gov/pubmed/22229090
http://dx.doi.org/10.1155/2011/502845
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author Kiyokawa, Iwao
Sogawa, Kazuyuki
Ise, Keiko
Iida, Fumie
Satoh, Mamoru
Miura, Toshihide
Kojima, Ryo
Katayama, Katsuhiro
Nomura, Fumio
author_facet Kiyokawa, Iwao
Sogawa, Kazuyuki
Ise, Keiko
Iida, Fumie
Satoh, Mamoru
Miura, Toshihide
Kojima, Ryo
Katayama, Katsuhiro
Nomura, Fumio
author_sort Kiyokawa, Iwao
collection PubMed
description One possible factor determining recovery of trace amount of protein biomarker candidates during proteome analyses could be adsorption on urine tubes. This issue, however, has not been well addressed so far. Recently, a new technical device of surface coating by poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)) (poly(MPC-co-BMA)) has been developed mainly to prevent the adsorption of plasma proteins. We assessed whether conventionally used urine tubes adsorb trace amount of urinary proteins and, if any, whether the surface coating by poly(MPC-co-BMA) can minimize the adsorption. Proteinuric urine samples were kept in poly(MPC-co-BMA)-coated and noncoated urine tubes for 15 min and possibly adsorbed proteins and/or peptides onto urine tubes were analyzed by SDS-PAGE, 2-DE, and the MALDI-TOF MS. It was found that a number of proteins and/or peptides adsorb on the conventionally used urine tubes and that surface coating by poly(MPC-co-BMA) can minimize the adsorption without any significant effects on routine urinalysis test results. Although it remains to be clarified to what extent the protein adsorption can modify the results of urinary proteome analyses, one has to consider this possible adsorption of urinary proteins when searching for trace amounts of protein biomarkers in urine.
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spelling pubmed-32001272012-01-06 Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes: Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating Kiyokawa, Iwao Sogawa, Kazuyuki Ise, Keiko Iida, Fumie Satoh, Mamoru Miura, Toshihide Kojima, Ryo Katayama, Katsuhiro Nomura, Fumio Int J Proteomics Research Article One possible factor determining recovery of trace amount of protein biomarker candidates during proteome analyses could be adsorption on urine tubes. This issue, however, has not been well addressed so far. Recently, a new technical device of surface coating by poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)) (poly(MPC-co-BMA)) has been developed mainly to prevent the adsorption of plasma proteins. We assessed whether conventionally used urine tubes adsorb trace amount of urinary proteins and, if any, whether the surface coating by poly(MPC-co-BMA) can minimize the adsorption. Proteinuric urine samples were kept in poly(MPC-co-BMA)-coated and noncoated urine tubes for 15 min and possibly adsorbed proteins and/or peptides onto urine tubes were analyzed by SDS-PAGE, 2-DE, and the MALDI-TOF MS. It was found that a number of proteins and/or peptides adsorb on the conventionally used urine tubes and that surface coating by poly(MPC-co-BMA) can minimize the adsorption without any significant effects on routine urinalysis test results. Although it remains to be clarified to what extent the protein adsorption can modify the results of urinary proteome analyses, one has to consider this possible adsorption of urinary proteins when searching for trace amounts of protein biomarkers in urine. Hindawi Publishing Corporation 2011 2011-09-12 /pmc/articles/PMC3200127/ /pubmed/22229090 http://dx.doi.org/10.1155/2011/502845 Text en Copyright © 2011 Iwao Kiyokawa et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kiyokawa, Iwao
Sogawa, Kazuyuki
Ise, Keiko
Iida, Fumie
Satoh, Mamoru
Miura, Toshihide
Kojima, Ryo
Katayama, Katsuhiro
Nomura, Fumio
Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes: Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating
title Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes: Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating
title_full Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes: Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating
title_fullStr Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes: Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating
title_full_unstemmed Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes: Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating
title_short Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes: Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating
title_sort adsorption of urinary proteins on the conventionally used urine collection tubes: possible effects on urinary proteome analysis and prevention of the adsorption by polymer coating
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3200127/
https://www.ncbi.nlm.nih.gov/pubmed/22229090
http://dx.doi.org/10.1155/2011/502845
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