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Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins

Mechanism of chemoprevention by daidzein (500 μg/g bwt) was examined by injecting it subcutaneously at 16th, 18th, and 20th day postpartum, followed by counting of terminal end buds (TEBs), terminal ducts (TDs), and lobules and immunohistochemistry of ER-α, Bcl2, Bax, and caspase-3. DNA fragmentatio...

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Autores principales: Mishra, Prachi, Kar, Anand, Kale, R. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scholarly Research Network 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3200189/
https://www.ncbi.nlm.nih.gov/pubmed/22091435
http://dx.doi.org/10.5402/2011/896826
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author Mishra, Prachi
Kar, Anand
Kale, R. K.
author_facet Mishra, Prachi
Kar, Anand
Kale, R. K.
author_sort Mishra, Prachi
collection PubMed
description Mechanism of chemoprevention by daidzein (500 μg/g bwt) was examined by injecting it subcutaneously at 16th, 18th, and 20th day postpartum, followed by counting of terminal end buds (TEBs), terminal ducts (TDs), and lobules and immunohistochemistry of ER-α, Bcl2, Bax, and caspase-3. DNA fragmentation was also analysed to measure the apoptosis level. Estradiol benzoate (EB) (500 ng/g bwt) and dimethyl sulphoxide (DMSO) were used as reference and vehicle, respectively. Observations show a significant enhancement of mammary gland differentiation at postnatal day 21 (PND21) as well as PND50. There was a significant decrease of ER-α expression at PND21 and increase in its expression at PND50, in daidzein-treated animals. The ratio of expression of Bcl-2 to Bax proteins increased at PND50 the same whereas, it decreased at PND50 due to daidzein. An increased expression of caspase-3 and DNA fragmentation was also seen due to daidzein at PND50. The mammary gland of EB-treated animals showed response a somewhat similar to that of daidzein-treated animals.
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spelling pubmed-32001892011-11-16 Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins Mishra, Prachi Kar, Anand Kale, R. K. ISRN Oncol Research Article Mechanism of chemoprevention by daidzein (500 μg/g bwt) was examined by injecting it subcutaneously at 16th, 18th, and 20th day postpartum, followed by counting of terminal end buds (TEBs), terminal ducts (TDs), and lobules and immunohistochemistry of ER-α, Bcl2, Bax, and caspase-3. DNA fragmentation was also analysed to measure the apoptosis level. Estradiol benzoate (EB) (500 ng/g bwt) and dimethyl sulphoxide (DMSO) were used as reference and vehicle, respectively. Observations show a significant enhancement of mammary gland differentiation at postnatal day 21 (PND21) as well as PND50. There was a significant decrease of ER-α expression at PND21 and increase in its expression at PND50, in daidzein-treated animals. The ratio of expression of Bcl-2 to Bax proteins increased at PND50 the same whereas, it decreased at PND50 due to daidzein. An increased expression of caspase-3 and DNA fragmentation was also seen due to daidzein at PND50. The mammary gland of EB-treated animals showed response a somewhat similar to that of daidzein-treated animals. International Scholarly Research Network 2011 2011-09-04 /pmc/articles/PMC3200189/ /pubmed/22091435 http://dx.doi.org/10.5402/2011/896826 Text en Copyright © 2011 Prachi Mishra et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mishra, Prachi
Kar, Anand
Kale, R. K.
Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins
title Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins
title_full Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins
title_fullStr Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins
title_full_unstemmed Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins
title_short Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins
title_sort prepubertal daidzein exposure enhances mammary gland differentiation and regulates the expression of estrogen receptor-alpha and apoptotic proteins
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3200189/
https://www.ncbi.nlm.nih.gov/pubmed/22091435
http://dx.doi.org/10.5402/2011/896826
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