Cargando…
Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins
Mechanism of chemoprevention by daidzein (500 μg/g bwt) was examined by injecting it subcutaneously at 16th, 18th, and 20th day postpartum, followed by counting of terminal end buds (TEBs), terminal ducts (TDs), and lobules and immunohistochemistry of ER-α, Bcl2, Bax, and caspase-3. DNA fragmentatio...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scholarly Research Network
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3200189/ https://www.ncbi.nlm.nih.gov/pubmed/22091435 http://dx.doi.org/10.5402/2011/896826 |
_version_ | 1782214667350835200 |
---|---|
author | Mishra, Prachi Kar, Anand Kale, R. K. |
author_facet | Mishra, Prachi Kar, Anand Kale, R. K. |
author_sort | Mishra, Prachi |
collection | PubMed |
description | Mechanism of chemoprevention by daidzein (500 μg/g bwt) was examined by injecting it subcutaneously at 16th, 18th, and 20th day postpartum, followed by counting of terminal end buds (TEBs), terminal ducts (TDs), and lobules and immunohistochemistry of ER-α, Bcl2, Bax, and caspase-3. DNA fragmentation was also analysed to measure the apoptosis level. Estradiol benzoate (EB) (500 ng/g bwt) and dimethyl sulphoxide (DMSO) were used as reference and vehicle, respectively. Observations show a significant enhancement of mammary gland differentiation at postnatal day 21 (PND21) as well as PND50. There was a significant decrease of ER-α expression at PND21 and increase in its expression at PND50, in daidzein-treated animals. The ratio of expression of Bcl-2 to Bax proteins increased at PND50 the same whereas, it decreased at PND50 due to daidzein. An increased expression of caspase-3 and DNA fragmentation was also seen due to daidzein at PND50. The mammary gland of EB-treated animals showed response a somewhat similar to that of daidzein-treated animals. |
format | Online Article Text |
id | pubmed-3200189 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | International Scholarly Research Network |
record_format | MEDLINE/PubMed |
spelling | pubmed-32001892011-11-16 Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins Mishra, Prachi Kar, Anand Kale, R. K. ISRN Oncol Research Article Mechanism of chemoprevention by daidzein (500 μg/g bwt) was examined by injecting it subcutaneously at 16th, 18th, and 20th day postpartum, followed by counting of terminal end buds (TEBs), terminal ducts (TDs), and lobules and immunohistochemistry of ER-α, Bcl2, Bax, and caspase-3. DNA fragmentation was also analysed to measure the apoptosis level. Estradiol benzoate (EB) (500 ng/g bwt) and dimethyl sulphoxide (DMSO) were used as reference and vehicle, respectively. Observations show a significant enhancement of mammary gland differentiation at postnatal day 21 (PND21) as well as PND50. There was a significant decrease of ER-α expression at PND21 and increase in its expression at PND50, in daidzein-treated animals. The ratio of expression of Bcl-2 to Bax proteins increased at PND50 the same whereas, it decreased at PND50 due to daidzein. An increased expression of caspase-3 and DNA fragmentation was also seen due to daidzein at PND50. The mammary gland of EB-treated animals showed response a somewhat similar to that of daidzein-treated animals. International Scholarly Research Network 2011 2011-09-04 /pmc/articles/PMC3200189/ /pubmed/22091435 http://dx.doi.org/10.5402/2011/896826 Text en Copyright © 2011 Prachi Mishra et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Mishra, Prachi Kar, Anand Kale, R. K. Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins |
title | Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins |
title_full | Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins |
title_fullStr | Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins |
title_full_unstemmed | Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins |
title_short | Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins |
title_sort | prepubertal daidzein exposure enhances mammary gland differentiation and regulates the expression of estrogen receptor-alpha and apoptotic proteins |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3200189/ https://www.ncbi.nlm.nih.gov/pubmed/22091435 http://dx.doi.org/10.5402/2011/896826 |
work_keys_str_mv | AT mishraprachi prepubertaldaidzeinexposureenhancesmammaryglanddifferentiationandregulatestheexpressionofestrogenreceptoralphaandapoptoticproteins AT karanand prepubertaldaidzeinexposureenhancesmammaryglanddifferentiationandregulatestheexpressionofestrogenreceptoralphaandapoptoticproteins AT kalerk prepubertaldaidzeinexposureenhancesmammaryglanddifferentiationandregulatestheexpressionofestrogenreceptoralphaandapoptoticproteins |