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RF1 Knockout Allows Ribosomal Incorporation of Unnatural Amino Acids at Multiple Sites

Stop codons have been exploited for genetic incorporation of unnatural amino acids (Uaas) in live cells, but the efficiency is low possibly due to competition from release factors, limiting the power and scope of this technology. Here we show that the reportedly essential release factor 1 can be kno...

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Detalles Bibliográficos
Autores principales:	Johnson, David B.F., Xu, Jianfeng, Shen, Zhouxin, Takimoto, Jeffrey K., Schultz, Matthew D., Schmitz, Robert J., Ecker, Joseph R., Briggs, Steven P., Wang, Lei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2011
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3201715/ https://www.ncbi.nlm.nih.gov/pubmed/21926996 http://dx.doi.org/10.1038/nchembio.657

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