Quantitative single cell monitoring of protein synthesis at subcellular resolution using fluorescently labeled tRNA

We have developed a novel technique of using fluorescent tRNA for translation monitoring (FtTM). FtTM enables the identification and monitoring of active protein synthesis sites within live cells at submicron resolution through quantitative microscopy of transfected bulk uncharged tRNA, fluorescentl...

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Detalles Bibliográficos
Autores principales: Barhoom, Sima, Kaur, Jaskiran, Cooperman, Barry S., Smorodinsky, Nechama I., Smilansky, Zeev, Ehrlich, Marcelo, Elroy-Stein, Orna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3201886/
https://www.ncbi.nlm.nih.gov/pubmed/21795382
http://dx.doi.org/10.1093/nar/gkr601

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3201886/
https://www.ncbi.nlm.nih.gov/pubmed/21795382
http://dx.doi.org/10.1093/nar/gkr601

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