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Epigenetic Changes with Dietary Soy in Cynomolgus Monkeys

Nutritional interventions are important alternatives for reducing the prevalence of many chronic diseases. Soy is a good source of protein that contains isoflavones, including genistein and daidzein, and may alter the risk of obesity, Type 2 diabetes, osteoporosis, cardiovascular disease, and reprod...

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Autores principales: Howard, Timothy D., Ho, Shuk-Mei, Zhang, Li, Chen, Jing, Cui, Wei, Slager, Rebecca, Gray, Stanton, Hawkins, Gregory A., Medvedovic, Mario, Wagner, Janice D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3201974/
https://www.ncbi.nlm.nih.gov/pubmed/22046358
http://dx.doi.org/10.1371/journal.pone.0026791
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author Howard, Timothy D.
Ho, Shuk-Mei
Zhang, Li
Chen, Jing
Cui, Wei
Slager, Rebecca
Gray, Stanton
Hawkins, Gregory A.
Medvedovic, Mario
Wagner, Janice D.
author_facet Howard, Timothy D.
Ho, Shuk-Mei
Zhang, Li
Chen, Jing
Cui, Wei
Slager, Rebecca
Gray, Stanton
Hawkins, Gregory A.
Medvedovic, Mario
Wagner, Janice D.
author_sort Howard, Timothy D.
collection PubMed
description Nutritional interventions are important alternatives for reducing the prevalence of many chronic diseases. Soy is a good source of protein that contains isoflavones, including genistein and daidzein, and may alter the risk of obesity, Type 2 diabetes, osteoporosis, cardiovascular disease, and reproductive cancers. We have shown previously in nonhuman primates that soy protein containing isoflavones leads to improved body weight, insulin sensitivity, lipid profiles, and atherosclerosis compared to protein without soy isoflavones (casein), and does not increase the risk of cancer. Since genistein has been shown to alter DNA methylation, we compared the methylation profiles of cynomolgus monkeys, from multiple tissues, eating two high-fat, typical American diets (TAD) with similar macronutrient contents, with or without soy protein. DNA methylation status was successfully determined for 80.6% of the probes in at least one tissue using Illumina's HumanMethylation27 BeadChip. Overall methylation increased in liver and muscle tissue when monkeys switched from the TAD-soy to the TAD-casein diets. Genes involved in epigenetic processes, specifically homeobox genes (HOXA5, HOXA11, and HOXB1), and ABCG5 were among those that changed between diets. These data support the use of the HumanMethylation27 BeadChip in cynomolgus monkeys and identify epigenetic changes associated with dietary interventions with soy protein that may potentially affect the etiology of complex diseases.
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spelling pubmed-32019742011-11-01 Epigenetic Changes with Dietary Soy in Cynomolgus Monkeys Howard, Timothy D. Ho, Shuk-Mei Zhang, Li Chen, Jing Cui, Wei Slager, Rebecca Gray, Stanton Hawkins, Gregory A. Medvedovic, Mario Wagner, Janice D. PLoS One Research Article Nutritional interventions are important alternatives for reducing the prevalence of many chronic diseases. Soy is a good source of protein that contains isoflavones, including genistein and daidzein, and may alter the risk of obesity, Type 2 diabetes, osteoporosis, cardiovascular disease, and reproductive cancers. We have shown previously in nonhuman primates that soy protein containing isoflavones leads to improved body weight, insulin sensitivity, lipid profiles, and atherosclerosis compared to protein without soy isoflavones (casein), and does not increase the risk of cancer. Since genistein has been shown to alter DNA methylation, we compared the methylation profiles of cynomolgus monkeys, from multiple tissues, eating two high-fat, typical American diets (TAD) with similar macronutrient contents, with or without soy protein. DNA methylation status was successfully determined for 80.6% of the probes in at least one tissue using Illumina's HumanMethylation27 BeadChip. Overall methylation increased in liver and muscle tissue when monkeys switched from the TAD-soy to the TAD-casein diets. Genes involved in epigenetic processes, specifically homeobox genes (HOXA5, HOXA11, and HOXB1), and ABCG5 were among those that changed between diets. These data support the use of the HumanMethylation27 BeadChip in cynomolgus monkeys and identify epigenetic changes associated with dietary interventions with soy protein that may potentially affect the etiology of complex diseases. Public Library of Science 2011-10-25 /pmc/articles/PMC3201974/ /pubmed/22046358 http://dx.doi.org/10.1371/journal.pone.0026791 Text en Howard et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Howard, Timothy D.
Ho, Shuk-Mei
Zhang, Li
Chen, Jing
Cui, Wei
Slager, Rebecca
Gray, Stanton
Hawkins, Gregory A.
Medvedovic, Mario
Wagner, Janice D.
Epigenetic Changes with Dietary Soy in Cynomolgus Monkeys
title Epigenetic Changes with Dietary Soy in Cynomolgus Monkeys
title_full Epigenetic Changes with Dietary Soy in Cynomolgus Monkeys
title_fullStr Epigenetic Changes with Dietary Soy in Cynomolgus Monkeys
title_full_unstemmed Epigenetic Changes with Dietary Soy in Cynomolgus Monkeys
title_short Epigenetic Changes with Dietary Soy in Cynomolgus Monkeys
title_sort epigenetic changes with dietary soy in cynomolgus monkeys
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3201974/
https://www.ncbi.nlm.nih.gov/pubmed/22046358
http://dx.doi.org/10.1371/journal.pone.0026791
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