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Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis

The heterotrimeric G-protein complex is minimally composed of Gα, Gβ, and Gγ subunits. In the classic scenario, the G-protein complex is the nexus in signaling from the plasma membrane, where the heterotrimeric G-protein associates with heptahelical G-protein-coupled receptors (GPCRs), to cytoplasmi...

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Autores principales: Klopffleisch, Karsten, Phan, Nguyen, Augustin, Kelsey, Bayne, Robert S, Booker, Katherine S, Botella, Jose R, Carpita, Nicholas C, Carr, Tyrell, Chen, Jin-Gui, Cooke, Thomas Ryan, Frick-Cheng, Arwen, Friedman, Erin J, Fulk, Brandon, Hahn, Michael G, Jiang, Kun, Jorda, Lucia, Kruppe, Lydia, Liu, Chenggang, Lorek, Justine, McCann, Maureen C, Molina, Antonio, Moriyama, Etsuko N, Mukhtar, M Shahid, Mudgil, Yashwanti, Pattathil, Sivakumar, Schwarz, John, Seta, Steven, Tan, Matthew, Temp, Ulrike, Trusov, Yuri, Urano, Daisuke, Welter, Bastian, Yang, Jing, Panstruga, Ralph, Uhrig, Joachim F, Jones, Alan M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3202803/
https://www.ncbi.nlm.nih.gov/pubmed/21952135
http://dx.doi.org/10.1038/msb.2011.66
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author Klopffleisch, Karsten
Phan, Nguyen
Augustin, Kelsey
Bayne, Robert S
Booker, Katherine S
Botella, Jose R
Carpita, Nicholas C
Carr, Tyrell
Chen, Jin-Gui
Cooke, Thomas Ryan
Frick-Cheng, Arwen
Friedman, Erin J
Fulk, Brandon
Hahn, Michael G
Jiang, Kun
Jorda, Lucia
Kruppe, Lydia
Liu, Chenggang
Lorek, Justine
McCann, Maureen C
Molina, Antonio
Moriyama, Etsuko N
Mukhtar, M Shahid
Mudgil, Yashwanti
Pattathil, Sivakumar
Schwarz, John
Seta, Steven
Tan, Matthew
Temp, Ulrike
Trusov, Yuri
Urano, Daisuke
Welter, Bastian
Yang, Jing
Panstruga, Ralph
Uhrig, Joachim F
Jones, Alan M
author_facet Klopffleisch, Karsten
Phan, Nguyen
Augustin, Kelsey
Bayne, Robert S
Booker, Katherine S
Botella, Jose R
Carpita, Nicholas C
Carr, Tyrell
Chen, Jin-Gui
Cooke, Thomas Ryan
Frick-Cheng, Arwen
Friedman, Erin J
Fulk, Brandon
Hahn, Michael G
Jiang, Kun
Jorda, Lucia
Kruppe, Lydia
Liu, Chenggang
Lorek, Justine
McCann, Maureen C
Molina, Antonio
Moriyama, Etsuko N
Mukhtar, M Shahid
Mudgil, Yashwanti
Pattathil, Sivakumar
Schwarz, John
Seta, Steven
Tan, Matthew
Temp, Ulrike
Trusov, Yuri
Urano, Daisuke
Welter, Bastian
Yang, Jing
Panstruga, Ralph
Uhrig, Joachim F
Jones, Alan M
author_sort Klopffleisch, Karsten
collection PubMed
description The heterotrimeric G-protein complex is minimally composed of Gα, Gβ, and Gγ subunits. In the classic scenario, the G-protein complex is the nexus in signaling from the plasma membrane, where the heterotrimeric G-protein associates with heptahelical G-protein-coupled receptors (GPCRs), to cytoplasmic target proteins called effectors. Although a number of effectors are known in metazoans and fungi, none of these are predicted to exist in their canonical forms in plants. To identify ab initio plant G-protein effectors and scaffold proteins, we screened a set of proteins from the G-protein complex using two-hybrid complementation in yeast. After deep and exhaustive interrogation, we detected 544 interactions between 434 proteins, of which 68 highly interconnected proteins form the core G-protein interactome. Within this core, over half of the interactions comprising two-thirds of the nodes were retested and validated as genuine in planta. Co-expression analysis in combination with phenotyping of loss-of-function mutations in a set of core interactome genes revealed a novel role for G-proteins in regulating cell wall modification.
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spelling pubmed-32028032011-10-27 Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis Klopffleisch, Karsten Phan, Nguyen Augustin, Kelsey Bayne, Robert S Booker, Katherine S Botella, Jose R Carpita, Nicholas C Carr, Tyrell Chen, Jin-Gui Cooke, Thomas Ryan Frick-Cheng, Arwen Friedman, Erin J Fulk, Brandon Hahn, Michael G Jiang, Kun Jorda, Lucia Kruppe, Lydia Liu, Chenggang Lorek, Justine McCann, Maureen C Molina, Antonio Moriyama, Etsuko N Mukhtar, M Shahid Mudgil, Yashwanti Pattathil, Sivakumar Schwarz, John Seta, Steven Tan, Matthew Temp, Ulrike Trusov, Yuri Urano, Daisuke Welter, Bastian Yang, Jing Panstruga, Ralph Uhrig, Joachim F Jones, Alan M Mol Syst Biol Report The heterotrimeric G-protein complex is minimally composed of Gα, Gβ, and Gγ subunits. In the classic scenario, the G-protein complex is the nexus in signaling from the plasma membrane, where the heterotrimeric G-protein associates with heptahelical G-protein-coupled receptors (GPCRs), to cytoplasmic target proteins called effectors. Although a number of effectors are known in metazoans and fungi, none of these are predicted to exist in their canonical forms in plants. To identify ab initio plant G-protein effectors and scaffold proteins, we screened a set of proteins from the G-protein complex using two-hybrid complementation in yeast. After deep and exhaustive interrogation, we detected 544 interactions between 434 proteins, of which 68 highly interconnected proteins form the core G-protein interactome. Within this core, over half of the interactions comprising two-thirds of the nodes were retested and validated as genuine in planta. Co-expression analysis in combination with phenotyping of loss-of-function mutations in a set of core interactome genes revealed a novel role for G-proteins in regulating cell wall modification. Nature Publishing Group 2011-09-27 /pmc/articles/PMC3202803/ /pubmed/21952135 http://dx.doi.org/10.1038/msb.2011.66 Text en Copyright © 2011, EMBO and Macmillan Publishers Limited http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial Share Alike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission.
spellingShingle Report
Klopffleisch, Karsten
Phan, Nguyen
Augustin, Kelsey
Bayne, Robert S
Booker, Katherine S
Botella, Jose R
Carpita, Nicholas C
Carr, Tyrell
Chen, Jin-Gui
Cooke, Thomas Ryan
Frick-Cheng, Arwen
Friedman, Erin J
Fulk, Brandon
Hahn, Michael G
Jiang, Kun
Jorda, Lucia
Kruppe, Lydia
Liu, Chenggang
Lorek, Justine
McCann, Maureen C
Molina, Antonio
Moriyama, Etsuko N
Mukhtar, M Shahid
Mudgil, Yashwanti
Pattathil, Sivakumar
Schwarz, John
Seta, Steven
Tan, Matthew
Temp, Ulrike
Trusov, Yuri
Urano, Daisuke
Welter, Bastian
Yang, Jing
Panstruga, Ralph
Uhrig, Joachim F
Jones, Alan M
Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis
title Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis
title_full Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis
title_fullStr Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis
title_full_unstemmed Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis
title_short Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis
title_sort arabidopsis g-protein interactome reveals connections to cell wall carbohydrates and morphogenesis
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3202803/
https://www.ncbi.nlm.nih.gov/pubmed/21952135
http://dx.doi.org/10.1038/msb.2011.66
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