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Role of the Mannose Receptor (CD206) in Innate Immunity to Ricin Toxin

The entry of ricin toxin into macrophages and certain other cell types in the spleen and liver results in toxin-induced inflammation, tissue damage and organ failure. It has been proposed that uptake of ricin into macrophages is facilitated by the mannose receptor (MR; CD206), a C-type lectin known...

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Detalles Bibliográficos
Autores principales: Gage, Emily, Hernandez, Maria O., O’Hara, Joanne M., McCarthy, Elizabeth A., Mantis, Nicholas J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3202876/
https://www.ncbi.nlm.nih.gov/pubmed/22069759
http://dx.doi.org/10.3390/toxins3091131
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author Gage, Emily
Hernandez, Maria O.
O’Hara, Joanne M.
McCarthy, Elizabeth A.
Mantis, Nicholas J.
author_facet Gage, Emily
Hernandez, Maria O.
O’Hara, Joanne M.
McCarthy, Elizabeth A.
Mantis, Nicholas J.
author_sort Gage, Emily
collection PubMed
description The entry of ricin toxin into macrophages and certain other cell types in the spleen and liver results in toxin-induced inflammation, tissue damage and organ failure. It has been proposed that uptake of ricin into macrophages is facilitated by the mannose receptor (MR; CD206), a C-type lectin known to recognize the oligosaccharide side chains on ricin’s A (RTA) and B (RTB) subunits. In this study, we confirmed that the MR does indeed promote ricin binding, uptake and killing of monocytes in vitro. To assess the role of MR in the pathogenesis of ricin in vivo, MR knockout (MR(−/−)) mice were challenged with the equivalent of 2.5× or 5× LD(50) of ricin by intraperitoneal injection. We found that MR(−/−) mice were significantly more susceptible to toxin-induced death than their age-matched, wild-type control counterparts. These data are consistent with a role for the MR in scavenging and degradation of ricin, not facilitating its uptake and toxicity in vivo.
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spelling pubmed-32028762011-11-08 Role of the Mannose Receptor (CD206) in Innate Immunity to Ricin Toxin Gage, Emily Hernandez, Maria O. O’Hara, Joanne M. McCarthy, Elizabeth A. Mantis, Nicholas J. Toxins (Basel) Article The entry of ricin toxin into macrophages and certain other cell types in the spleen and liver results in toxin-induced inflammation, tissue damage and organ failure. It has been proposed that uptake of ricin into macrophages is facilitated by the mannose receptor (MR; CD206), a C-type lectin known to recognize the oligosaccharide side chains on ricin’s A (RTA) and B (RTB) subunits. In this study, we confirmed that the MR does indeed promote ricin binding, uptake and killing of monocytes in vitro. To assess the role of MR in the pathogenesis of ricin in vivo, MR knockout (MR(−/−)) mice were challenged with the equivalent of 2.5× or 5× LD(50) of ricin by intraperitoneal injection. We found that MR(−/−) mice were significantly more susceptible to toxin-induced death than their age-matched, wild-type control counterparts. These data are consistent with a role for the MR in scavenging and degradation of ricin, not facilitating its uptake and toxicity in vivo. MDPI 2011-09-09 /pmc/articles/PMC3202876/ /pubmed/22069759 http://dx.doi.org/10.3390/toxins3091131 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Gage, Emily
Hernandez, Maria O.
O’Hara, Joanne M.
McCarthy, Elizabeth A.
Mantis, Nicholas J.
Role of the Mannose Receptor (CD206) in Innate Immunity to Ricin Toxin
title Role of the Mannose Receptor (CD206) in Innate Immunity to Ricin Toxin
title_full Role of the Mannose Receptor (CD206) in Innate Immunity to Ricin Toxin
title_fullStr Role of the Mannose Receptor (CD206) in Innate Immunity to Ricin Toxin
title_full_unstemmed Role of the Mannose Receptor (CD206) in Innate Immunity to Ricin Toxin
title_short Role of the Mannose Receptor (CD206) in Innate Immunity to Ricin Toxin
title_sort role of the mannose receptor (cd206) in innate immunity to ricin toxin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3202876/
https://www.ncbi.nlm.nih.gov/pubmed/22069759
http://dx.doi.org/10.3390/toxins3091131
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