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Mechanical Loading of Articular Cartilage Reduces IL-1-Induced Enzyme Expression
OBJECTIVE: Exposure of articular cartilage to interleukin-1 (IL-1) results in increased synthesis of matrix degrading enzymes. Previously mechanical load applied together with IL-1 stimulation was found to reduce aggrecan cleavage by ADAMTS-4 and 5 and MMP-1, -3, -9, and -13 and reduce proteoglycan...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203026/ https://www.ncbi.nlm.nih.gov/pubmed/22039566 http://dx.doi.org/10.1177/1947603511407484 |
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author | Torzilli, P. A. Bhargava, M. Chen, C. T. |
author_facet | Torzilli, P. A. Bhargava, M. Chen, C. T. |
author_sort | Torzilli, P. A. |
collection | PubMed |
description | OBJECTIVE: Exposure of articular cartilage to interleukin-1 (IL-1) results in increased synthesis of matrix degrading enzymes. Previously mechanical load applied together with IL-1 stimulation was found to reduce aggrecan cleavage by ADAMTS-4 and 5 and MMP-1, -3, -9, and -13 and reduce proteoglycan loss from the extracellular matrix. To further delineate the inhibition mechanism the gene expression of ADAMTS-4 and 5; MMP-1, -3, -9, and -13; and TIMP-1, -2, and -3 were measured. DESIGN: Mature bovine articular cartilage was stimulated with a 0.5 MPa compressive stress and 10 ng/ml of IL-1α for 3 days and then allowed to recover without stimulation for 1 additional day. The media was assayed for proteoglycan content on a daily basis, while chondrocyte gene expression (mRNA) was measured during stimulation and 1 day of recovery. RESULTS: Mechanical load alone did not change the gene expression for ADAMTS, MMP, or TIMP. IL-1 caused an increase in gene expression for all enzymes after 1 day of stimulation while not affecting the TIMP levels. Load applied together with IL-1 decreased the expression levels of ADAMTS-4 and -5 and MMP-1 and -3 and increased TIMP-3 expression. CONCLUSIONS: A mechanical load appears to modify cartilage degradation by IL-1 at the cellular level by reducing mRNA. |
format | Online Article Text |
id | pubmed-3203026 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-32030262012-10-01 Mechanical Loading of Articular Cartilage Reduces IL-1-Induced Enzyme Expression Torzilli, P. A. Bhargava, M. Chen, C. T. Cartilage Original Articles OBJECTIVE: Exposure of articular cartilage to interleukin-1 (IL-1) results in increased synthesis of matrix degrading enzymes. Previously mechanical load applied together with IL-1 stimulation was found to reduce aggrecan cleavage by ADAMTS-4 and 5 and MMP-1, -3, -9, and -13 and reduce proteoglycan loss from the extracellular matrix. To further delineate the inhibition mechanism the gene expression of ADAMTS-4 and 5; MMP-1, -3, -9, and -13; and TIMP-1, -2, and -3 were measured. DESIGN: Mature bovine articular cartilage was stimulated with a 0.5 MPa compressive stress and 10 ng/ml of IL-1α for 3 days and then allowed to recover without stimulation for 1 additional day. The media was assayed for proteoglycan content on a daily basis, while chondrocyte gene expression (mRNA) was measured during stimulation and 1 day of recovery. RESULTS: Mechanical load alone did not change the gene expression for ADAMTS, MMP, or TIMP. IL-1 caused an increase in gene expression for all enzymes after 1 day of stimulation while not affecting the TIMP levels. Load applied together with IL-1 decreased the expression levels of ADAMTS-4 and -5 and MMP-1 and -3 and increased TIMP-3 expression. CONCLUSIONS: A mechanical load appears to modify cartilage degradation by IL-1 at the cellular level by reducing mRNA. SAGE Publications 2011-10 /pmc/articles/PMC3203026/ /pubmed/22039566 http://dx.doi.org/10.1177/1947603511407484 Text en © The Author(s) 2011 |
spellingShingle | Original Articles Torzilli, P. A. Bhargava, M. Chen, C. T. Mechanical Loading of Articular Cartilage Reduces IL-1-Induced Enzyme Expression |
title | Mechanical Loading of Articular Cartilage Reduces IL-1-Induced Enzyme Expression |
title_full | Mechanical Loading of Articular Cartilage Reduces IL-1-Induced Enzyme Expression |
title_fullStr | Mechanical Loading of Articular Cartilage Reduces IL-1-Induced Enzyme Expression |
title_full_unstemmed | Mechanical Loading of Articular Cartilage Reduces IL-1-Induced Enzyme Expression |
title_short | Mechanical Loading of Articular Cartilage Reduces IL-1-Induced Enzyme Expression |
title_sort | mechanical loading of articular cartilage reduces il-1-induced enzyme expression |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203026/ https://www.ncbi.nlm.nih.gov/pubmed/22039566 http://dx.doi.org/10.1177/1947603511407484 |
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