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Anaplasmataceae and Borrelia burgdorferi sensu lato in the sand lizard Lacerta agilis and co-infection of these bacteria in hosted Ixodes ricinus ticks

BACKGROUND: Anaplasmataceae and Borrelia burgdorferi s.l. are important tick-borne bacteria maintained in nature by transmission between ticks and vertebrate hosts. However, the potential role of lizards as hosts has not been sufficiently studied. RESULTS: The current study showed that 23 of 171 exa...

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Autores principales: Ekner, Anna, Dudek, Krzysztof, Sajkowska, Zofia, Majláthová, Viktória, Majláth, Igor, Tryjanowski, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203261/
https://www.ncbi.nlm.nih.gov/pubmed/21933412
http://dx.doi.org/10.1186/1756-3305-4-182
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author Ekner, Anna
Dudek, Krzysztof
Sajkowska, Zofia
Majláthová, Viktória
Majláth, Igor
Tryjanowski, Piotr
author_facet Ekner, Anna
Dudek, Krzysztof
Sajkowska, Zofia
Majláthová, Viktória
Majláth, Igor
Tryjanowski, Piotr
author_sort Ekner, Anna
collection PubMed
description BACKGROUND: Anaplasmataceae and Borrelia burgdorferi s.l. are important tick-borne bacteria maintained in nature by transmission between ticks and vertebrate hosts. However, the potential role of lizards as hosts has not been sufficiently studied. RESULTS: The current study showed that 23 of 171 examined sand lizards Lacerta agilis were PCR positive for Anaplasmataceae. The nucleotide sequences of the several selected PCR products showed 100% homology with Anaplasma spp. found in Ixodes ricinus collected in Tunisia and Morocco (AY672415 - AY672420). 1.2% of lizard collar scale samples were PCR positive for B. lusitaniae. In addition, 12 of 290 examined I. ricinus were PCR positive for B. burgdorferi s.l. and 82 were PCR positive for Anaplasmatacea. The number of ticks per lizard and the number of ticks PCR positive for both microorganisms per lizard were strongly correlated. Moreover, we found a significant correlation between numbers of ticks infected with Anaplasmataceae and with B. burgdorferi s.l. living on the same lizard. However, there was no significant correlation between detection of both bacteria in the same tick. CONCLUSIONS: To the best of our knowledge, this is the first report of Anaplasmataceae DNA and additionally the second report of B. burgdorferi s.l DNA detection in the sand lizard.
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spelling pubmed-32032612011-10-31 Anaplasmataceae and Borrelia burgdorferi sensu lato in the sand lizard Lacerta agilis and co-infection of these bacteria in hosted Ixodes ricinus ticks Ekner, Anna Dudek, Krzysztof Sajkowska, Zofia Majláthová, Viktória Majláth, Igor Tryjanowski, Piotr Parasit Vectors Research BACKGROUND: Anaplasmataceae and Borrelia burgdorferi s.l. are important tick-borne bacteria maintained in nature by transmission between ticks and vertebrate hosts. However, the potential role of lizards as hosts has not been sufficiently studied. RESULTS: The current study showed that 23 of 171 examined sand lizards Lacerta agilis were PCR positive for Anaplasmataceae. The nucleotide sequences of the several selected PCR products showed 100% homology with Anaplasma spp. found in Ixodes ricinus collected in Tunisia and Morocco (AY672415 - AY672420). 1.2% of lizard collar scale samples were PCR positive for B. lusitaniae. In addition, 12 of 290 examined I. ricinus were PCR positive for B. burgdorferi s.l. and 82 were PCR positive for Anaplasmatacea. The number of ticks per lizard and the number of ticks PCR positive for both microorganisms per lizard were strongly correlated. Moreover, we found a significant correlation between numbers of ticks infected with Anaplasmataceae and with B. burgdorferi s.l. living on the same lizard. However, there was no significant correlation between detection of both bacteria in the same tick. CONCLUSIONS: To the best of our knowledge, this is the first report of Anaplasmataceae DNA and additionally the second report of B. burgdorferi s.l DNA detection in the sand lizard. BioMed Central 2011-09-20 /pmc/articles/PMC3203261/ /pubmed/21933412 http://dx.doi.org/10.1186/1756-3305-4-182 Text en Copyright ©2011 Ekner et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Ekner, Anna
Dudek, Krzysztof
Sajkowska, Zofia
Majláthová, Viktória
Majláth, Igor
Tryjanowski, Piotr
Anaplasmataceae and Borrelia burgdorferi sensu lato in the sand lizard Lacerta agilis and co-infection of these bacteria in hosted Ixodes ricinus ticks
title Anaplasmataceae and Borrelia burgdorferi sensu lato in the sand lizard Lacerta agilis and co-infection of these bacteria in hosted Ixodes ricinus ticks
title_full Anaplasmataceae and Borrelia burgdorferi sensu lato in the sand lizard Lacerta agilis and co-infection of these bacteria in hosted Ixodes ricinus ticks
title_fullStr Anaplasmataceae and Borrelia burgdorferi sensu lato in the sand lizard Lacerta agilis and co-infection of these bacteria in hosted Ixodes ricinus ticks
title_full_unstemmed Anaplasmataceae and Borrelia burgdorferi sensu lato in the sand lizard Lacerta agilis and co-infection of these bacteria in hosted Ixodes ricinus ticks
title_short Anaplasmataceae and Borrelia burgdorferi sensu lato in the sand lizard Lacerta agilis and co-infection of these bacteria in hosted Ixodes ricinus ticks
title_sort anaplasmataceae and borrelia burgdorferi sensu lato in the sand lizard lacerta agilis and co-infection of these bacteria in hosted ixodes ricinus ticks
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203261/
https://www.ncbi.nlm.nih.gov/pubmed/21933412
http://dx.doi.org/10.1186/1756-3305-4-182
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