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Insights into a Multidrug Resistant Escherichia coli Pathogen of the Globally Disseminated ST131 Lineage: Genome Analysis and Virulence Mechanisms

Escherichia coli strains causing urinary tract infection (UTI) are increasingly recognized as belonging to specific clones. E. coli clone O25b:H4-ST131 has recently emerged globally as a leading multi-drug resistant pathogen causing urinary tract and bloodstream infections in hospitals and the commu...

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Autores principales: Totsika, Makrina, Beatson, Scott A., Sarkar, Sohinee, Phan, Minh-Duy, Petty, Nicola K., Bachmann, Nathan, Szubert, Marek, Sidjabat, Hanna E., Paterson, David L., Upton, Mathew, Schembri, Mark A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203889/
https://www.ncbi.nlm.nih.gov/pubmed/22053197
http://dx.doi.org/10.1371/journal.pone.0026578
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author Totsika, Makrina
Beatson, Scott A.
Sarkar, Sohinee
Phan, Minh-Duy
Petty, Nicola K.
Bachmann, Nathan
Szubert, Marek
Sidjabat, Hanna E.
Paterson, David L.
Upton, Mathew
Schembri, Mark A.
author_facet Totsika, Makrina
Beatson, Scott A.
Sarkar, Sohinee
Phan, Minh-Duy
Petty, Nicola K.
Bachmann, Nathan
Szubert, Marek
Sidjabat, Hanna E.
Paterson, David L.
Upton, Mathew
Schembri, Mark A.
author_sort Totsika, Makrina
collection PubMed
description Escherichia coli strains causing urinary tract infection (UTI) are increasingly recognized as belonging to specific clones. E. coli clone O25b:H4-ST131 has recently emerged globally as a leading multi-drug resistant pathogen causing urinary tract and bloodstream infections in hospitals and the community. While most molecular studies to date examine the mechanisms conferring multi-drug resistance in E. coli ST131, relatively little is known about their virulence potential. Here we examined E. coli ST131 clinical isolates from two geographically diverse collections, one representing the major pathogenic lineages causing UTI across the United Kingdom and a second representing UTI isolates from patients presenting at two large hospitals in Australia. We determined a draft genome sequence for one representative isolate, E. coli EC958, which produced CTX-M-15 extended-spectrum β-lactamase, CMY-23 type AmpC cephalosporinase and was resistant to ciprofloxacin. Comparative genome analysis indicated that EC958 encodes virulence genes commonly associated with uropathogenic E. coli (UPEC). The genome sequence of EC958 revealed a transposon insertion in the fimB gene encoding the activator of type 1 fimbriae, an important UPEC bladder colonization factor. We identified the same fimB transposon insertion in 59% of the ST131 UK isolates, as well as 71% of ST131 isolates from Australia, suggesting this mutation is common among E. coli ST131 strains. Insertional inactivation of fimB resulted in a phenotype resembling a slower off-to-on switching for type 1 fimbriae. Type 1 fimbriae expression could still be induced in fimB-null isolates; this correlated strongly with adherence to and invasion of human bladder cells and bladder colonisation in a mouse UTI model. We conclude that E. coli ST131 is a geographically widespread, antibiotic resistant clone that has the capacity to produce numerous virulence factors associated with UTI.
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spelling pubmed-32038892011-11-03 Insights into a Multidrug Resistant Escherichia coli Pathogen of the Globally Disseminated ST131 Lineage: Genome Analysis and Virulence Mechanisms Totsika, Makrina Beatson, Scott A. Sarkar, Sohinee Phan, Minh-Duy Petty, Nicola K. Bachmann, Nathan Szubert, Marek Sidjabat, Hanna E. Paterson, David L. Upton, Mathew Schembri, Mark A. PLoS One Research Article Escherichia coli strains causing urinary tract infection (UTI) are increasingly recognized as belonging to specific clones. E. coli clone O25b:H4-ST131 has recently emerged globally as a leading multi-drug resistant pathogen causing urinary tract and bloodstream infections in hospitals and the community. While most molecular studies to date examine the mechanisms conferring multi-drug resistance in E. coli ST131, relatively little is known about their virulence potential. Here we examined E. coli ST131 clinical isolates from two geographically diverse collections, one representing the major pathogenic lineages causing UTI across the United Kingdom and a second representing UTI isolates from patients presenting at two large hospitals in Australia. We determined a draft genome sequence for one representative isolate, E. coli EC958, which produced CTX-M-15 extended-spectrum β-lactamase, CMY-23 type AmpC cephalosporinase and was resistant to ciprofloxacin. Comparative genome analysis indicated that EC958 encodes virulence genes commonly associated with uropathogenic E. coli (UPEC). The genome sequence of EC958 revealed a transposon insertion in the fimB gene encoding the activator of type 1 fimbriae, an important UPEC bladder colonization factor. We identified the same fimB transposon insertion in 59% of the ST131 UK isolates, as well as 71% of ST131 isolates from Australia, suggesting this mutation is common among E. coli ST131 strains. Insertional inactivation of fimB resulted in a phenotype resembling a slower off-to-on switching for type 1 fimbriae. Type 1 fimbriae expression could still be induced in fimB-null isolates; this correlated strongly with adherence to and invasion of human bladder cells and bladder colonisation in a mouse UTI model. We conclude that E. coli ST131 is a geographically widespread, antibiotic resistant clone that has the capacity to produce numerous virulence factors associated with UTI. Public Library of Science 2011-10-28 /pmc/articles/PMC3203889/ /pubmed/22053197 http://dx.doi.org/10.1371/journal.pone.0026578 Text en Totsika et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Totsika, Makrina
Beatson, Scott A.
Sarkar, Sohinee
Phan, Minh-Duy
Petty, Nicola K.
Bachmann, Nathan
Szubert, Marek
Sidjabat, Hanna E.
Paterson, David L.
Upton, Mathew
Schembri, Mark A.
Insights into a Multidrug Resistant Escherichia coli Pathogen of the Globally Disseminated ST131 Lineage: Genome Analysis and Virulence Mechanisms
title Insights into a Multidrug Resistant Escherichia coli Pathogen of the Globally Disseminated ST131 Lineage: Genome Analysis and Virulence Mechanisms
title_full Insights into a Multidrug Resistant Escherichia coli Pathogen of the Globally Disseminated ST131 Lineage: Genome Analysis and Virulence Mechanisms
title_fullStr Insights into a Multidrug Resistant Escherichia coli Pathogen of the Globally Disseminated ST131 Lineage: Genome Analysis and Virulence Mechanisms
title_full_unstemmed Insights into a Multidrug Resistant Escherichia coli Pathogen of the Globally Disseminated ST131 Lineage: Genome Analysis and Virulence Mechanisms
title_short Insights into a Multidrug Resistant Escherichia coli Pathogen of the Globally Disseminated ST131 Lineage: Genome Analysis and Virulence Mechanisms
title_sort insights into a multidrug resistant escherichia coli pathogen of the globally disseminated st131 lineage: genome analysis and virulence mechanisms
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203889/
https://www.ncbi.nlm.nih.gov/pubmed/22053197
http://dx.doi.org/10.1371/journal.pone.0026578
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