Cargando…
Effect of methyl methanesulfonate on hsp70 expression and tissue damage in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg (9)
Methyl methanesulfonate (MMS) is an anti-carcinogenic drug and its toxicity has been reported in various experimental models. The hsp70s are a family of ubiquitously expressed heat shock proteins. In the recent years, hsp70 has been considered to be one of the candidate genes for predicting cytotoxi...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Slovak Toxicology Society SETOX
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203919/ https://www.ncbi.nlm.nih.gov/pubmed/22058658 http://dx.doi.org/10.2478/v10102-011-0025-7 |
_version_ | 1782215154520293376 |
---|---|
author | Kumar, Vineet Ara, Gulshan Afzal, Mohammad Siddique, Yasir Hasan |
author_facet | Kumar, Vineet Ara, Gulshan Afzal, Mohammad Siddique, Yasir Hasan |
author_sort | Kumar, Vineet |
collection | PubMed |
description | Methyl methanesulfonate (MMS) is an anti-carcinogenic drug and its toxicity has been reported in various experimental models. The hsp70s are a family of ubiquitously expressed heat shock proteins. In the recent years, hsp70 has been considered to be one of the candidate genes for predicting cytotoxicity against environmental chemicals. Nowadays emphasis is given to the use of alternatives to mammals in testing, research and education. The European Centre for the Validation of Alternative Methods (EVCAM) has recommended the use of Drosophila as an alternative model for scientific studies. Almost all living organisms possess proteins with a similar structure to that of hsp70s. In the present study, the toxicity of MMS was evaluated by quantifying hsp70 expression and tissue damage in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg (9), at different doses and hours of exposure. We studied the effect of 0.25, 0.50, 0.75 and 1.0 µl/ml of MMS at 2, 4, 24 and 48 hours of exposure on hsp70 expression by using the soluble O-nitrophenyl-β-D-galactopyranoside (ONPG) assay and on establishing the tissue damage by the Trypan blue exclusion assay in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg (9). A dose-dependent increase in the expression of hsp70 was observed at 0.25, 0.50, and 0.75 µl/ml of MMS compared to the control. At the highest dose, i.e. 1.0 µl/ml of MMS, the activity of hsp70 was decreased due to tissue damage. |
format | Online Article Text |
id | pubmed-3203919 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Slovak Toxicology Society SETOX |
record_format | MEDLINE/PubMed |
spelling | pubmed-32039192011-11-06 Effect of methyl methanesulfonate on hsp70 expression and tissue damage in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg (9) Kumar, Vineet Ara, Gulshan Afzal, Mohammad Siddique, Yasir Hasan Interdiscip Toxicol Original Article Methyl methanesulfonate (MMS) is an anti-carcinogenic drug and its toxicity has been reported in various experimental models. The hsp70s are a family of ubiquitously expressed heat shock proteins. In the recent years, hsp70 has been considered to be one of the candidate genes for predicting cytotoxicity against environmental chemicals. Nowadays emphasis is given to the use of alternatives to mammals in testing, research and education. The European Centre for the Validation of Alternative Methods (EVCAM) has recommended the use of Drosophila as an alternative model for scientific studies. Almost all living organisms possess proteins with a similar structure to that of hsp70s. In the present study, the toxicity of MMS was evaluated by quantifying hsp70 expression and tissue damage in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg (9), at different doses and hours of exposure. We studied the effect of 0.25, 0.50, 0.75 and 1.0 µl/ml of MMS at 2, 4, 24 and 48 hours of exposure on hsp70 expression by using the soluble O-nitrophenyl-β-D-galactopyranoside (ONPG) assay and on establishing the tissue damage by the Trypan blue exclusion assay in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg (9). A dose-dependent increase in the expression of hsp70 was observed at 0.25, 0.50, and 0.75 µl/ml of MMS compared to the control. At the highest dose, i.e. 1.0 µl/ml of MMS, the activity of hsp70 was decreased due to tissue damage. Slovak Toxicology Society SETOX 2011-09 2011-09 /pmc/articles/PMC3203919/ /pubmed/22058658 http://dx.doi.org/10.2478/v10102-011-0025-7 Text en Copyright © 2011 Slovak Toxicology Society SETOX http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kumar, Vineet Ara, Gulshan Afzal, Mohammad Siddique, Yasir Hasan Effect of methyl methanesulfonate on hsp70 expression and tissue damage in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg (9) |
title | Effect of methyl methanesulfonate on hsp70 expression and tissue damage in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg
(9)
|
title_full | Effect of methyl methanesulfonate on hsp70 expression and tissue damage in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg
(9)
|
title_fullStr | Effect of methyl methanesulfonate on hsp70 expression and tissue damage in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg
(9)
|
title_full_unstemmed | Effect of methyl methanesulfonate on hsp70 expression and tissue damage in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg
(9)
|
title_short | Effect of methyl methanesulfonate on hsp70 expression and tissue damage in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg
(9)
|
title_sort | effect of methyl methanesulfonate on hsp70 expression and tissue damage in the third instar larvae of transgenic drosophila melanogaster (hsp70-lacz) bg
(9) |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203919/ https://www.ncbi.nlm.nih.gov/pubmed/22058658 http://dx.doi.org/10.2478/v10102-011-0025-7 |
work_keys_str_mv | AT kumarvineet effectofmethylmethanesulfonateonhsp70expressionandtissuedamageinthethirdinstarlarvaeoftransgenicdrosophilamelanogasterhsp70laczbg9 AT aragulshan effectofmethylmethanesulfonateonhsp70expressionandtissuedamageinthethirdinstarlarvaeoftransgenicdrosophilamelanogasterhsp70laczbg9 AT afzalmohammad effectofmethylmethanesulfonateonhsp70expressionandtissuedamageinthethirdinstarlarvaeoftransgenicdrosophilamelanogasterhsp70laczbg9 AT siddiqueyasirhasan effectofmethylmethanesulfonateonhsp70expressionandtissuedamageinthethirdinstarlarvaeoftransgenicdrosophilamelanogasterhsp70laczbg9 |